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Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:,

Enzymes and Genes:
nucleoside triphosphate pyrophosphohydrolase: mazG

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name for nucleoside-triphosphate diphosphatase

Enzyme Commission Synonyms for nucleoside-triphosphate pyrophosphatase

Enzyme Commission Primary Name for (d)CTP diphosphatase

Enzyme Commission Synonyms for (d)CTP pyrophosphohydrolase, (d)CTP diphosphohydrolase, nudG (gene name)

Enzyme Commission Summary for
May be identical with EC nucleotide diphosphatase.

Enzyme Commission Summary for
The enzyme, characterized from the bacterium Escherichia coli, is specific for the pyrimidine nucleotides CTP and dCTP. It also acts on 5-methyl-dCTP, 5-hydroxy-CTP and 8-hydroxy-dGTP.

Citations: [Chern69, Zhang02c, OHandley01, Fujikawa02, Iida05]

Gene-Reaction Schematic

Gene-Reaction Schematic

Instance reactions of [a nucleoside triphosphate + H2O → a nucleoside 5'-monophosphate + diphosphate + H+] (
i1: dGTP + H2O → dGMP + diphosphate + H+ (

i2: dATP + H2O → dAMP + diphosphate + H+ (

i3: dUTP + H2O → dUMP + diphosphate + H+ (

i4: XTP + H2O → XMP + diphosphate + H+ (

i5: CTP + H2O → CMP + diphosphate + H+ (
i6: dITP + H2O → dIMP + diphosphate + H+ (

i7: ATP + H2O → AMP + diphosphate + H+ (

i8: ITP + H2O → IMP + diphosphate + H+ (

i9: dCTP + H2O → dCMP + diphosphate + H+ (

i10: UTP + H2O → UMP + diphosphate + H+ (

i11: dTTP + H2O → dTMP + diphosphate + H+ (

Relationship Links: BRENDA:EC:, BRENDA:EC:, ENZYME:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:, IUBMB-ExplorEnz:EC:


Chern69: Chern CJ, MacDonald AB, Morris AJ (1969). "Purification and properties of a nucleoside triphosphate pyrophosphohydrolase from red cells of the rabbit." J Biol Chem 244(20);5489-95. PMID: 4310599

Fujikawa02: Fujikawa K, Kasai H (2002). "The oxidized pyrimidine ribonucleotide, 5-hydroxy-CTP, is hydrolyzed efficiently by the Escherichia coli recombinant Orf135 protein." DNA Repair (Amst) 1(7);571-6. PMID: 12509230

Iida05: Iida E, Satou K, Mishima M, Kojima C, Harashima H, Kamiya H (2005). "Amino acid residues involved in substrate recognition of the Escherichia coli Orf135 protein." Biochemistry 44(15);5683-9. PMID: 15823026

OHandley01: O'Handley SF, Dunn CA, Bessman MJ (2001). "Orf135 from Escherichia coli Is a Nudix hydrolase specific for CTP, dCTP, and 5-methyl-dCTP." J Biol Chem 2001;276(8);5421-6. PMID: 11053429

Zhang02c: Zhang J, Inouye M (2002). "MazG, a nucleoside triphosphate pyrophosphohydrolase, interacts with Era, an essential GTPase in Escherichia coli." J Bacteriol 184(19);5323-9. PMID: 12218018

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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