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Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeComplex Processes
Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
α-D-xyloside xylohydrolaseInferred from experiment: yicI

Note that this reaction equation differs from the official Enzyme Commission reaction equation for this EC number, which can be found here .

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: α-D-xyloside xylohydrolase

Enzyme Commission Synonyms: α-xylosidase

Enzyme Commission Summary:
The enzyme catalyses hydrolysis of a terminal, unsubstituted xyloside at the extreme reducing end of a xylogluco-oligosaccharide. Representative α-xylosidases from glycoside hydrolase family 31 utilize a two-step (double-displacement) mechanism involving a covalent glycosyl-enzyme intermediate, and retain the anomeric configuration of the product.

Citations: [Moracci00 , Sampedro01, Crombie02, Lovering05, Iglesias05, Okuyama06, Larsbrink11]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Created 13-May-2011 by Caspi R, SRI International


Crombie02: Crombie HJ, Chengappa S, Jarman C, Sidebottom C, Reid JS (2002). "Molecular characterisation of a xyloglucan oligosaccharide-acting alpha-D-xylosidase from nasturtium (Tropaeolum majus L.) cotyledons that resembles plant 'apoplastic' alpha-D-glucosidases." Planta 214(3);406-13. PMID: 11859845

Iglesias05: Iglesias N, Abelenda JA, Rodino M, Sampedro J, Revilla G, Zarra I (2005). "Apoplastic Glycosidases Active Against Xyloglucan Oligosaccharides of Arabidopsis thaliana." Plant Cell Physiol NIL. PMID: 16267099

Larsbrink11: Larsbrink J, Izumi A, Ibatullin FM, Nakhai A, Gilbert HJ, Davies GJ, Brumer H (2011). "Structural and enzymatic characterization of a glycoside hydrolase family 31 α-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification." Biochem J 436(3);567-80. PMID: 21426303

Lovering05: Lovering AL, Lee SS, Kim YW, Withers SG, Strynadka NC (2005). "Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate." J Biol Chem 280(3);2105-15. PMID: 15501829

Moracci00: Moracci M, Cobucci Ponzano B, Trincone A, Fusco S, De Rosa M, van Der Oost J, Sensen CW, Charlebois RL, Rossi M (2000). "Identification and molecular characterization of the first alpha -xylosidase from an archaeon." J Biol Chem 275(29);22082-9. PMID: 10801892

Okuyama06: Okuyama M, Kaneko A, Mori H, Chiba S, Kimura A (2006). "Structural elements to convert Escherichia coli alpha-xylosidase (YicI) into alpha-glucosidase." FEBS Lett 580(11);2707-11. PMID: 16631751

Sampedro01: Sampedro J, Sieiro C, Revilla G, Gonzalez-Villa T, Zarra I (2001). "Cloning and expression pattern of a gene encoding an alpha-xylosidase active against xyloglucan oligosaccharides from Arabidopsis." Plant Physiol 126(2);910-20. PMID: 11402218

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.5 on Fri Feb 12, 2016, BIOCYC11A.