Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
EcoKI restriction-modification systemInferred from experiment: hsdM, hsdR, hsdS

Supersedes EC numbers:,

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Marked as unbalanced.

Enzyme Commission Primary Name: type I site-specific deoxyribonuclease

Enzyme Commission Synonyms: type I restriction enzyme, deoxyribonuclease (ATP- and S-adenosyl-L-methionine-dependent), restriction-modification system, deoxyribonuclease (adenosine triphosphate-hydrolyzing), adenosine triphosphate-dependent deoxyribonuclease, ATP-dependent DNase, type 1 site-specific deoxyribonuclease

Enzyme Commission Summary:
This enzyme catalyzes endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolysed.

This is a large group of enzymes which, together with those now listed as EC (type II site-specific deoxyribonuclease) and EC (type III site-specific deoxyribonuclease), were previously listed separately in sub-subclasses EC 3.1.23 and EC 3.1.24. They have an absolute requirement for ATP (or dATP) and S-adenosyl-L-methionine. They recognize specific short DNA sequences and cleave at sites remote from the recognition sequence. They are multifunctional proteins that also catalyse the reactions of EC [site-specific DNA-methyltransferase (adenine-specific)] and EC

Citations: [Roberts90a]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Roberts90a: Roberts RJ (1990). "Restriction enzymes and their isoschizomers." Nucleic Acids Res 18 Suppl;2331-65. PMID: 2159140

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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