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Escherichia coli K-12 substr. MG1655 Reaction: 3.1.21.3

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 3.1.21.3

Enzymes and Genes:
EcoKI restriction-modification system Inferred from experiment : hsdM , hsdR , hsdS

Supersedes EC numbers: 3.1.24.2, 3.1.24.1

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Marked as unbalanced.

Enzyme Commission Primary Name: type I site-specific deoxyribonuclease

Enzyme Commission Synonyms: type I restriction enzyme, deoxyribonuclease (ATP- and S-adenosyl-L-methionine-dependent), restriction-modification system, deoxyribonuclease (adenosine triphosphate-hydrolyzing), adenosine triphosphate-dependent deoxyribonuclease, ATP-dependent DNase, type 1 site-specific deoxyribonuclease

Enzyme Commission Summary:
This enzyme catalyzes endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolysed.

This is a large group of enzymes which, together with those now listed as EC 3.1.21.4 (type II site-specific deoxyribonuclease) and EC 3.1.21.5 (type III site-specific deoxyribonuclease), were previously listed separately in sub-subclasses EC 3.1.23 and EC 3.1.24. They have an absolute requirement for ATP (or dATP) and S-adenosyl-L-methionine. They recognize specific short DNA sequences and cleave at sites remote from the recognition sequence. They are multifunctional proteins that also catalyse the reactions of EC 2.1.1.72 [site-specific DNA-methyltransferase (adenine-specific)] and EC 2.1.1.37.

Citations: [Roberts90]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:3.1.21.3 , ENZYME:EC:3.1.21.3 , IUBMB-ExplorEnz:EC:3.1.21.3


References

Roberts90: Roberts RJ (1990). "Restriction enzymes and their isoschizomers." Nucleic Acids Res 18 Suppl;2331-65. PMID: 2159140


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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