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Escherichia coli K-12 substr. MG1655 Polypeptide: aliphatic sulfonate ABC transporter - membrane subunit




Gene: ssuC Accession Numbers: G6476 (EcoCyc), b0934, ECK0925

Synonyms: ycbM

Regulation Summary Diagram

Regulation summary diagram for ssuC

Component of: aliphatic sulfonate ABC transporter (extended summary available)

Summary:
Based on sequence analysis ssuC encodes the integral membrane component of an aliphatic sulfonate ABC transporter [vanDer99]. An ΔssuC ΔssuB strain is unable to grow using a range of aliphatic sulfonates as sulfur source including MOPS, HEPES, ethanesulfonate, hexanesulfonate, octanesulfonate, decanesulfonate and sulfoacetate. Wild type growth is restored when ssuC and ssuB are expressed from a plasmid [Eichhorn00].

SsuC contains 6-7 predicted transmembrane helices; the N and C-termini are located in the cytoplasm [Drew02].

Locations: inner membrane

Map Position: [993,264 <- 994,055] (21.41 centisomes, 77°)
Length: 792 bp / 263 aa

Molecular Weight of Polypeptide: 28.925 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003175, EchoBASE:EB3469, EcoGene:EG13705, EcoliWiki:b0934, OU-Microarray:b0934, PortEco:ssuC, Pride:P75851, Protein Model Portal:P75851, RefSeq:NP_415454, RegulonDB:G6476, String:511145.b0934, UniProt:P75851

Relationship Links: InterPro:IN-FAMILY:IPR000515, Pfam:IN-FAMILY:PF00528, Prosite:IN-FAMILY:PS50928

In Paralogous Gene Group: 24 (43 members)

Gene-Reaction Schematic

Gene-Reaction Schematic

Genetic Regulation Schematic

Genetic regulation schematic for ssuC


GO Terms:
Biological Process:
Inferred from experimentGO:0006790 - sulfur compound metabolic process [vanDer99]
Inferred from experimentGO:0042918 - alkanesulfonate transport [vanDer99, Eichhorn00]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a, GOA01a]
Molecular Function:
Inferred from experimentGO:0042959 - alkanesulfonate transporter activity [vanDer99, Eichhorn00]
Cellular Component:
Inferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09]
Inferred by computational analysisGO:0005887 - integral component of plasma membrane [Drew02]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]
Inferred by computational analysisGO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing [vanDer99]

MultiFun Terms: cell structuremembrane
metabolismmetabolism of other compoundsphosphorous metabolism
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, membrane component

Essentiality data for ssuC knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: aliphatic sulfonate ABC transporter

Synonyms: YcbE/YcbM ABC transporter, alkanesulfonate ABC transporter

Subunit composition of aliphatic sulfonate ABC transporter = [SsuA][SsuC]2[SsuB]2
         aliphatic sulfonate ABC transporter - periplasmic binding protein = SsuA (summary available)
         aliphatic sulfonate ABC transporter - membrane subunit = SsuC (summary available)
         aliphatic sulfonate ABC transporter - ATP binding subunit = SsuB (summary available)

Summary:
The ssuEADCB gene cluster codes for proteins that enable Escherichia coli to utilize a broad range of sulfonates as a source of sulfur.

A strain lacking the chromosomal ssuEADBC gene cluster is unable to use a broad range of aliphatic sulfonates as sulfur source including ethanesulfonate, propanesulfonate, butanesulfonate, pentanesulfonate, hexanesulfonate, ethanedisulfonate, octanesulfonate, decanesulfonate, isethionate, sulfoacetate, MOPS, HEPES, MES, and PIPES but is able to use sulfur from sulfate, cysteine, cystine, lanthionine, methionine, glutathione and taurine [vanDer99]. Deletion of the ssu transporter gene cluster results in the inability to use 4-phenyl-1-butanesulfonate, 2-(4-pyridyl)ethanesulfonate, 3-aminopropanesulfonate, and N-phenyltaurine as a sulfur source [Eichhorn00].

Based on sequence analysis SsuABC is an ABC type transport system with SsuA being the periplasmic substrate-binding subunit, SsuB the ATP-binding subunit, and SsuC the permease [vanDer99]. ssuD and ssuE encode an FMNH2-dependent sulfonate monooxygenase and an NAD(P)H-dependent FMN reductase, respectively.

ssu: sulfonate sulfur utilization

Locations: inner membrane


GO Terms:
Biological Process:
Inferred from experimentGO:0042918 - alkanesulfonate transport [Eichhorn00, vanDer99]
Molecular Function:
Inferred from experimentGO:0042959 - alkanesulfonate transporter activity [Eichhorn00, vanDer99]
Inferred by computational analysisGO:0042626 - ATPase activity, coupled to transmembrane movement of substances [vanDer99]
Cellular Component:
Inferred by computational analysisGO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing [vanDer99]

Credits:
Last-Curated 01-Jul-2014 by Mackie A, Macquarie University


Enzymatic reaction of: transport of an aliphatic sulfonate (aliphatic sulfonate ABC transporter)

Inferred by computational analysisInferred from experiment

Transport reaction diagram for transport of an aliphatic sulfonate


Sequence Features

Protein sequence of aliphatic sulfonate ABC transporter - membrane subunit with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 14 -> 34
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 44 -> 64
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Conserved-Region 58 -> 242
Inferred by computational analysis[UniProt15]
UniProt: ABC transmembrane type-1.
Transmembrane-Region 69 -> 89
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Pfam PF00528 80 -> 247
Inferred by computational analysis[Finn14]
BPD_transp_1 : Binding-protein-dependent transport system inner membrane component
Transmembrane-Region 103 -> 122
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 126 -> 148
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 165 -> 185
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 188 -> 208
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 218 -> 238
Inferred by computational analysis[UniProt15]
UniProt: Helical.


Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Unit

Transcription-unit diagram

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Drew02: Drew D, Sjostrand D, Nilsson J, Urbig T, Chin CN, de Gier JW, von Heijne G (2002). "Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis." Proc Natl Acad Sci U S A 99(5);2690-5. PMID: 11867724

Eichhorn00: Eichhorn E, van der Ploeg JR, Leisinger T (2000). "Deletion analysis of the Escherichia coli taurine and alkanesulfonate transport systems." J Bacteriol 182(10);2687-95. PMID: 10781534

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

vanDer99: van Der Ploeg JR, Iwanicka-Nowicka R, Bykowski T, Hryniewicz MM, Leisinger T (1999). "The Escherichia coli ssuEADCB gene cluster is required for the utilization of sulfur from aliphatic sulfonates and is regulated by the transcriptional activator Cbl." J Biol Chem 274(41);29358-65. PMID: 10506196

Other References Related to Gene Regulation

Bykowski02: Bykowski T, van der Ploeg JR, Iwanicka-Nowicka R, Hryniewicz MM (2002). "The switch from inorganic to organic sulphur assimilation in Escherichia coli: adenosine 5'-phosphosulphate (APS) as a signalling molecule for sulphate excess." Mol Microbiol 43(5);1347-58. PMID: 11918818

Salmon03: Salmon K, Hung SP, Mekjian K, Baldi P, Hatfield GW, Gunsalus RP (2003). "Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR." J Biol Chem 278(32);29837-55. PMID: 12754220


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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