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Escherichia coli K-12 substr. MG1655 Polypeptide: ribose ABC transporter - putative ATP binding subunit



Gene: rbsA Accession Numbers: EG10814 (EcoCyc), b3749, ECK3743

Synonyms: rbsT, rbsP

Regulation Summary Diagram: ?

Component of: ribose ABC transporter (extended summary available)

Gene Citations: [Bell86, Burland93]

Locations: inner membrane, cytosol

Map Position: [3,931,801 -> 3,933,306] (84.74 centisomes)
Length: 1506 bp / 501 aa

Molecular Weight of Polypeptide: 55.042 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012259 , CGSC:12082 , DIP:DIP-10640N , EchoBASE:EB0807 , EcoGene:EG10814 , EcoliWiki:b3749 , ModBase:P04983 , OU-Microarray:b3749 , PortEco:rbsA , PR:PRO_000023682 , Pride:P04983 , Protein Model Portal:P04983 , RefSeq:NP_418205 , RegulonDB:EG10814 , SMR:P04983 , String:511145.b3749 , UniProt:P04983

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR015861 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Prosite:IN-FAMILY:PS51254 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
GO:0015749 - monosaccharide transport Inferred by computational analysis [GOA01]
GO:0015752 - D-ribose transport Inferred by computational analysis [GOA06]
GO:0034219 - carbohydrate transmembrane transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA06, GOA01a]
GO:0015407 - monosaccharide-transporting ATPase activity Inferred by computational analysis [GOA01]
GO:0015591 - D-ribose transmembrane transporter activity Inferred by computational analysis [GOA06]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01a]
GO:0043211 - carbohydrate-transporting ATPase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [GOA06]

MultiFun Terms: metabolism carbon utilization carbon compounds
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for rbsA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: ribose ABC transporter

Subunit composition of ribose ABC transporter = [RbsA]2[RbsC]2[RbsB]
         ribose ABC transporter - putative ATP binding subunit = RbsA
         ribose ABC transporter - membrane subunit = RbsC
         ribose ABC transporter - putative periplasmic binding protein = RbsB

Summary:
RbsACB is an ATP-dependent ribose transporter that is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Park99]. Based on sequence similarity, RbsA is the ATP-binding constituent, RbsB is the periplasmic substrate-binding protein, and RbsC form the transmembrane constituent of the transporter [Park99]. Mutations in each of the components eliminated transport of ribose at external concentration of 1 μM, indicating that the components make up a transport system that is responsible for high-affinity ribose transport. However, these mutants are able to grow normally on high concentrations of the sugar, suggesting that there is at least a second, low-affinity transport system for ribose in E. coli [Iida84]. Hydrophobicity analysis has shown that RbsC contains six transmembrane helices, while alkaline phosphatase fusions and the use of inside-out vesicles with proteolysis have shown that the C and N termini are both on the cytoplasmic side of the membrane.


Enzymatic reaction of: transport of D-ribose (ribose ABC transporter)

EC Number: 3.6.3.17


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 5 -> 241
[UniProt09]
UniProt: ABC transporter 1;
Nucleotide-Phosphate-Binding-Region 37 -> 44
[UniProt10a]
UniProt: ATP; Non-Experimental Qualifier: potential;
Mutagenesis-Variant 43
[Barroga96, UniProt11]
Alternate sequence: K → R; UniProt: Loss of transport.
Conserved-Region 252 -> 495
[UniProt09]
UniProt: ABC transporter 2;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b3749 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10814; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Barroga96: Barroga CF, Zhang H, Wajih N, Bouyer JH, Hermodson MA (1996). "The proteins encoded by the rbs operon of Escherichia coli: I. Overproduction, purification, characterization, and functional analysis of RbsA." Protein Sci 5(6);1093-9. PMID: 8762140

Bell86: Bell AW, Buckel SD, Groarke JM, Hope JN, Kingsley DH, Hermodson MA (1986). "The nucleotide sequences of the rbsD, rbsA, and rbsC genes of Escherichia coli K12." J Biol Chem 261(17);7652-8. PMID: 3011793

Burland93: Burland V, Plunkett G, Daniels DL, Blattner FR (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 1993;16(3);551-61. PMID: 7686882

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Iida84: Iida A, Harayama S, Iino T, Hazelbauer GL (1984). "Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12." J Bacteriol 158(2);674-82. PMID: 6327617

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Park99: Park Y, Park C (1999). "Topology of RbsC, a membrane component of the ribose transporter, belonging to the AraH superfamily." J Bacteriol 1999;181(3);1039-42. PMID: 9922273

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Mauzy92: Mauzy CA, Hermodson MA (1992). "Structural and functional analyses of the repressor, RbsR, of the ribose operon of Escherichia coli." Protein Sci 1992;1(7);831-42. PMID: 1304369

Shimada13a: Shimada T, Kori A, Ishihama A (2013). "Involvement of the ribose operon repressor RbsR in regulation of purine nucleotide synthesis in Escherichia coli." FEMS Microbiol Lett 344(2);159-65. PMID: 23651393

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, BIOCYC14B.