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Escherichia coli K-12 substr. MG1655 Protein: MngR DNA-binding transcriptional repressor



Gene: mngR Accession Numbers: EG11109 (EcoCyc), b0730, ECK0718

Synonyms: ybgB, farR

Regulation Summary Diagram: ?

Subunit composition of MngR DNA-binding transcriptional repressor = [MngR]2

Summary:
According to microarray analysis, the "mannosyl-d-glycerate regulator," MngR, regulates only two genes, mngA and mngB, involved in 2-O-α-mannosyl-D-glycerate utilization [Sampaio04]. This regulator also autoregulates its own synthesis [Quail94a].

Previously, MngR, also called FarR, for fatty acyl-responsive regulator, had been proposed to regulate genes involved in the citric acid cycle in response to fatty acids, which release MngR from its DNA-binding site [Quail94a]. However, when it was found that this protein only regulates two genes, the suggestion was rejected and it was proposed that probably high concentrations of fatty acids could degrade the protein instead of it binding to the regulator [Sampaio04].

MngR belongs to the GntR family of transcriptional regulators and has a helix-turn-helix motif in the N-terminal domain and a UbiC transcription regulator-associated domain that is predicted to be a sensor for small molecules. This domain is found in proteins such as UbiC from Escherichia coli, TreR from Bacillus subtilis, and HutC from Pseudomonas aeruginosa [Aravind03].

The mntR gene is transcribed divergently from the mngAB operon. They share a regulatory region that contains two sites of 21 bp each, one with two direct repeat sequences of 10 bp that are bound by MngR [Quail94a].

mngR shows differential codon adaptation, resulting in differential translation efficiency signatures, in thermophilic microbes. It was therefore predicted to play a role in the heat shock response. An mngR deletion mutant was shown to be more sensitive than wild-type specifically to heat shock, but not other stresses [Krisko14].

Locations: cytosol

Map Position: [764,376 <- 765,098] (16.47 centisomes)
Length: 723 bp / 240 aa

Molecular Weight of Polypeptide: 28.273 kD (from nucleotide sequence)

pI: 6.53

Unification Links: ASAP:ABE-0002487 , CGSC:36837 , DIP:DIP-9565N , EchoBASE:EB1100 , EcoGene:EG11109 , EcoliWiki:b0730 , ModBase:P13669 , OU-Microarray:b0730 , PortEco:mngR , PR:PRO_000023253 , Protein Model Portal:P13669 , RefSeq:NP_415258 , RegulonDB:EG11109 , SMR:P13669 , String:511145.b0730 , UniProt:P13669

Relationship Links: EcoO157Cyc:Homolog:Z4874 , EcoO157Cyc:Homolog:Z4874-MONOMER , InterPro:IN-FAMILY:IPR000524 , InterPro:IN-FAMILY:IPR011663 , InterPro:IN-FAMILY:IPR011991 , Pfam:IN-FAMILY:PF00392 , Pfam:IN-FAMILY:PF07702 , Prints:IN-FAMILY:PR00035 , Prosite:IN-FAMILY:PS50949 , Smart:IN-FAMILY:SM00345 , Smart:IN-FAMILY:SM00866

In Paralogous Gene Group: 186 (3 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0009408 - response to heat Inferred from experiment [Krisko14]
GO:0045892 - negative regulation of transcription, DNA-templated Inferred from experiment Inferred by computational analysis [Aravind03, Quail94a, Sampaio04]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a, GOA01]
Molecular Function: GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred from experiment Inferred by computational analysis [GOA01, Aravind03, Quail94a, Sampaio04]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11a, GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred by curator
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
metabolism energy metabolism, carbon TCA cycle
regulation type of regulation transcriptional level repressor

DNA binding site length: 22 base-pairs

Symmetry: Direct Repeat

Consensus DNA Binding Sequence: tATttgtaTTatATTtGTATTA

Regulated Transcription Units (2 total): ?

Notes:

Essentiality data for mngR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Conserved-Region 4 -> 72
[UniProt09]
UniProt: HTH gntR-type;
DNA-Binding-Region 32 -> 51
[UniProt10a]
UniProt: H-T-H motif; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b0730 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11109; confirmed by SwissProt match.


References

Aravind03: Aravind L, Anantharaman V (2003). "HutC/FarR-like bacterial transcription factors of the GntR family contain a small molecule-binding domain of the chorismate lyase fold." FEMS Microbiol Lett 222(1);17-23. PMID: 12757941

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Krisko14: Krisko A, Copi T, Gabaldon T, Lehner B, Supek F (2014). "Inferring gene function from evolutionary change in signatures of translation efficiency." Genome Biol 15(3);R44. PMID: 24580753

Quail94a: Quail MA, Dempsey CE, Guest JR (1994). "Identification of a fatty acyl responsive regulator (FarR) in Escherichia coli." FEBS Lett 1994;356(2-3);183-7. PMID: 7805834

Sampaio04: Sampaio MM, Chevance F, Dippel R, Eppler T, Schlegel A, Boos W, Lu YJ, Rock CO (2004). "Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor." J Biol Chem 279(7);5537-48. PMID: 14645248

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Buck89: Buck D, Guest JR (1989). "Overexpression and site-directed mutagenesis of the succinyl-CoA synthetase of Escherichia coli and nucleotide sequence of a gene (g30) that is adjacent to the suc operon." Biochem J 1989;260(3);737-47. PMID: 2548486


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, biocyc13.