|Gene:||mngR||Accession Numbers: EG11109 (EcoCyc), b0730, ECK0718|
Synonyms: ybgB, farR
Subunit composition of MngR DNA-binding transcriptional repressor = [MngR]2
According to microarray analysis, the "mannosyl-d-glycerate regulator," MngR, regulates only two genes, mngA and mngB, involved in 2-O-α-mannosyl-D-glycerate utilization [Sampaio04]. This regulator also autoregulates its own synthesis [Quail94a].
Previously, MngR, also called FarR, for fatty acyl-responsive regulator, had been proposed to regulate genes involved in the citric acid cycle in response to fatty acids, which release MngR from its DNA-binding site [Quail94a]. However, when it was found that this protein only regulates two genes, the suggestion was rejected and it was proposed that probably high concentrations of fatty acids could degrade the protein instead of it binding to the regulator [Sampaio04].
MngR belongs to the GntR family of transcriptional regulators and has a helix-turn-helix motif in the N-terminal domain and a UbiC transcription regulator-associated domain that is predicted to be a sensor for small molecules. This domain is found in proteins such as UbiC from Escherichia coli, TreR from Bacillus subtilis, and HutC from Pseudomonas aeruginosa [Aravind03].
The mntR gene is transcribed divergently from the mngAB operon. They share a regulatory region that contains two sites of 21 bp each, one with two direct repeat sequences of 10 bp that are bound by MngR [Quail94a].
mngR shows differential codon adaptation, resulting in differential translation efficiency signatures, in thermophilic microbes. It was therefore predicted to play a role in the heat shock response. An mngR deletion mutant was shown to be more sensitive than wild-type specifically to heat shock, but not other stresses [Krisko14].
|Map Position: [764,376 <- 765,098] (16.47 centisomes)||Length: 723 bp / 240 aa|
Molecular Weight of Polypeptide: 28.273 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0002487 , CGSC:36837 , DIP:DIP-9565N , EchoBASE:EB1100 , EcoGene:EG11109 , EcoliWiki:b0730 , ModBase:P13669 , OU-Microarray:b0730 , PortEco:mngR , PR:PRO_000023253 , Protein Model Portal:P13669 , RefSeq:NP_415258 , RegulonDB:EG11109 , SMR:P13669 , String:511145.b0730 , UniProt:P13669
Relationship Links: EcoO157Cyc:Homolog:Z4874 , EcoO157Cyc:Homolog:Z4874-MONOMER , InterPro:IN-FAMILY:IPR000524 , InterPro:IN-FAMILY:IPR011663 , InterPro:IN-FAMILY:IPR011991 , Pfam:IN-FAMILY:PF00392 , Pfam:IN-FAMILY:PF07702 , Prints:IN-FAMILY:PR00035 , Prosite:IN-FAMILY:PS50949 , Smart:IN-FAMILY:SM00345 , Smart:IN-FAMILY:SM00866
In Paralogous Gene Group: 186 (3 members)
|Biological Process:||GO:0009408 - response to heat
GO:0045892 - negative regulation of transcription, DNA-templated [Aravind03, Quail94a, Sampaio04]
GO:0006351 - transcription, DNA-templated [UniProtGOA11]
GO:0006355 - regulation of transcription, DNA-templated [UniProtGOA11, GOA01]
|Molecular Function:||GO:0003700 - sequence-specific DNA binding transcription factor activity
[GOA01, Aravind03, Quail94a, Sampaio04]
GO:0003677 - DNA binding [UniProtGOA11, GOA01]
|Cellular Component:||GO:0005737 - cytoplasm
GO:0005829 - cytosol [DiazMejia09]
|MultiFun Terms:||information transfer → RNA related → Transcription related|
|metabolism → energy metabolism, carbon → TCA cycle|
|regulation → type of regulation → transcriptional level → repressor|
DNA binding site length: 22 base-pairs
Symmetry: Direct Repeat
Consensus DNA Binding Sequence: tATttgtaTTatATTtGTATTA
|Growth Medium||Growth?||T (°C)||O2||pH||Osm/L||Growth Observations|
|LB enriched||Yes||37||Aerobic||6.95||Yes [Gerdes03, Comment 1]|
|LB Lennox||Yes||37||Aerobic||7||Yes [Baba06, Comment 2]|
|M9 medium with 1% glycerol||Yes||37||Aerobic||7.2||0.35||Yes [Joyce06, Comment 3]|
|MOPS medium with 0.4% glucose||Yes||37||Aerobic||7.2||0.22||Yes [Baba06, Comment 2]|
|Conserved-Region||4 -> 72|
|DNA-Binding-Region||32 -> 51|
10/20/97 Gene b0730 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11109; confirmed by SwissProt match.
Aravind03: Aravind L, Anantharaman V (2003). "HutC/FarR-like bacterial transcription factors of the GntR family contain a small molecule-binding domain of the chorismate lyase fold." FEMS Microbiol Lett 222(1);17-23. PMID: 12757941
Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938
Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394
Sampaio04: Sampaio MM, Chevance F, Dippel R, Eppler T, Schlegel A, Boos W, Lu YJ, Rock CO (2004). "Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor." J Biol Chem 279(7);5537-48. PMID: 14645248
Buck89: Buck D, Guest JR (1989). "Overexpression and site-directed mutagenesis of the succinyl-CoA synthetase of Escherichia coli and nucleotide sequence of a gene (g30) that is adjacent to the suc operon." Biochem J 1989;260(3);737-47. PMID: 2548486
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