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Escherichia coli K-12 substr. MG1655 Polypeptide: hydrogenase 1, b-type cytochrome subunit




Gene: hyaC Accession Numbers: EG10470 (EcoCyc), b0974, ECK0965

Synonyms: cybH, hydrogenase 1 - B subunit, hydrogenase 1 - γ subunit

Regulation Summary Diagram

Regulation summary diagram for hyaC

Component of:
hydrogenase 1
hydrogenase 1, oxygen tolerant hydrogenase (extended summary available)

Summary:
The hyaC gene product is very hydrophobic, rich in aromatic residues, and has four putative hydrophobic membrane-spanning regions [Menon90].

An in-frame deletion in the hyaC gene results in wild-type levels of hydrogenase 1 activity and the appearance of multiple forms of the enzyme during purification [Menon91]. Sequence analysis suggests that the HyaC subunit contains two heme groups but only one heme could be identified in the crystal structure of the hydrogenase 1 complex; HyaC's main function may be anchoring the hydrogenase to the membrane [Volbeda13].

Gene Citations: [Maciag11]

Locations: inner membrane

Map Position: [1,034,289 -> 1,034,996] (22.29 centisomes, 80°)
Length: 708 bp / 235 aa

Molecular Weight of Polypeptide: 27.597 kD (from nucleotide sequence), 26.0 kD (experimental) [Menon91]

pI: 9.42

Unification Links: ASAP:ABE-0003290, CGSC:31788, DIP:DIP-35859N, EchoBASE:EB0465, EcoGene:EG10470, EcoliWiki:b0974, ModBase:P0AAM1, OU-Microarray:b0974, PortEco:hyaC, Protein Model Portal:P0AAM1, RefSeq:NP_415493, RegulonDB:EG10470, SMR:P0AAM1, String:511145.b0974, UniProt:P0AAM1

Relationship Links: InterPro:IN-FAMILY:IPR000516, InterPro:IN-FAMILY:IPR011577, InterPro:IN-FAMILY:IPR016174, PDB:Structure:4GD3, Pfam:IN-FAMILY:PF00033, Prints:IN-FAMILY:PR00161, Prosite:IN-FAMILY:PS00882, Prosite:IN-FAMILY:PS00883

Reactions known to consume the compound:

Not in pathways:
a ubiquinol-8 oxidoreductase + a b-type cytochrome → a ubiquinone-8 oxidoreductase + a reduced b-type cytochrome
a b-type cytochrome + a menaquinone oxidoreductase (demethylmenaquinol) → a reduced b-type cytochrome + a menaquinone oxidoreductase (demethylmenaquinone)
a b-type cytochrome + a menaquinone oxidoreductase (menaquinol-8) → a reduced b-type cytochrome + a menaquinone oxidoreductase (menaquinone-8)
a reduced b-type cytochrome + a b-type cytochromea b-type cytochrome + a reduced cytochrome o

Gene-Reaction Schematic

Gene-Reaction Schematic

Genetic Regulation Schematic

Genetic regulation schematic for hyaC


GO Terms:
Biological Process:
Inferred from experimentGO:0006113 - fermentation [Brondsted94]
Inferred from experimentGO:0009061 - anaerobic respiration [Brondsted94]
Inferred by computational analysisGO:0006810 - transport [GOA01]
Inferred by curatorGO:0019645 - anaerobic electron transport chain [Laurinavichene01]
Inferred by computational analysisGO:0022904 - respiratory electron transport chain [GOA01]
Inferred by computational analysisGO:0055114 - oxidation-reduction process [UniProtGOA11]
Inferred by curatorGO:1902421 - hydrogen metabolic process [Laurinavichene01, Sawers86]
Molecular Function:
Inferred from experimentGO:0005515 - protein binding [Volbeda13]
Inferred from experimentGO:0020037 - heme binding [Volbeda13]
Inferred by computational analysisGO:0005506 - iron ion binding [GOA01]
Inferred by computational analysisGO:0009055 - electron carrier activity [GOA01]
Inferred by curatorGO:0033748 - hydrogenase (acceptor) activity [Sawers86]
Inferred by computational analysisGO:0046872 - metal ion binding [UniProtGOA11]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11a, UniProtGOA11, Daley05, DiazMejia09]
Inferred by computational analysisInferred from experimentGO:0005887 - integral component of plasma membrane [Volbeda13, Menon90]
Inferred from experimentGO:0044569 - [Ni-Fe] hydrogenase complex [Volbeda13]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11, GOA01]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11]

MultiFun Terms: cell structuremembrane
metabolismbiosynthesis of macromolecules (cellular constituents)large molecule carrierscytochromes
metabolismenergy metabolism, carbonaerobic respiration
metabolismenergy metabolism, carbonanaerobic respiration
metabolismenergy production/transportelectron donors

Essentiality data for hyaC knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]
Yes [Feist07, Comment 3]

Credits:
Last-Curated 26-Feb-2015 by Mackie A, Macquarie University


Subunit of: hydrogenase 1

Synonyms: HYD1, hydrogenase I, NiFe hydrogenase, hydrogen:menaquinone oxidoreductase, hydrogen oxidase, hydrogen:oxygen oxidoreductase

Subunit composition of hydrogenase 1 = [HyaA]2[HyaB]2[HyaC]
         hydrogenase 1, small subunit = HyaA (extended summary available)
         hydrogenase 1, large subunit = HyaB (summary available)
         hydrogenase 1, b-type cytochrome subunit = HyaC (summary available)

Component of: hydrogenase 1, oxygen tolerant hydrogenase (extended summary available)

Relationship Links: PDB:Structure:3UQY, PDB:Structure:3USC, PDB:Structure:3USE

Credits:
Revised 19-Feb-2015 by Mackie A, Macquarie University
Last-Curated 19-Feb-2015 by Mackie A, Macquarie University


Subunit of: hydrogenase 1, oxygen tolerant hydrogenase

Subunit composition of hydrogenase 1, oxygen tolerant hydrogenase = [(HyaA)2(HyaB)2(HyaC)]2
         hydrogenase 1 = (HyaA)2(HyaB)2(HyaC)
                 hydrogenase 1, small subunit = HyaA (extended summary available)
                 hydrogenase 1, large subunit = HyaB (summary available)
                 hydrogenase 1, b-type cytochrome subunit = HyaC (summary available)

Summary:
Hydrogenase I (encoded by the hyaABC genes in E. coli K-12) is an oxygen-tolerant, membrane bound Ni-Fe hydrogenase [Sawers86, Laurinavichene01]. The purified enzyme catalyses H2:benzyl viologen oxidoreduction; it also catalyses H2 evolution with methyl viologen as an electron donor [Sawers86]. In a strain lacking the hydrogenase 2 enzyme, hydrogenase 1 catalyses nitrate dependent H2 consumption and DMSO dependent H2 consumption but is less active with methyl viologen, benzyl viologen and fumarate. This mutant strain also demonstrates H2 consumption in the presence of air (ie. it is active in the Knallgas reaction 2H2 + O2 → 2H2O); hydrogenase 1 donates electrons preferentially to acceptors with higher reduction potential and is active at higher oxygen concentrations than hydrogenase 2 [Laurinavichene01, Laurinavichene02].

Purified hydrogenase 1 is a uni-directional, oxygen-tolerant H(2) oxidizer; hydrogenase 1 may function as an energy conserving, H(2) scavenger under conditions of slow growth and fluctuating oxygen levels [Lukey10]. Hydrogenase 1 can directly catalyse the 4 electron reduction of oxygen to water using H(2) as donor; when hydrogenase 1 reacts exclusively with H(2) and oxygen, 86% of the water produced arises from this direct reaction [Wulff14]. The oxidase activity of of hydrogenase 1 is low and serves to protect the active site from oxygen attack [Wulff14]

The membrane bound complex crystallises as a [HyaC(HyaAHyaB)2]2 complex and this is thought to be the physiologically relevant structure. Hydrogenase I is anchored to the membrane by both a HyaA C-terminal helix and an inner membrane b-type cytochome, HyaC; cytochrome b's main role may be anchoring the heterodimer (HyaAB) to the membrane. The small subunit (HyaA) contains a distal [4Fe-4S]cluster, a medial [3Fe-4S] cluster and a unique proximal [4Fe-3S] cluster; the large subunit (HyaB) contains the Ni-Fe active site [Volbeda12, Volbeda13].

The basis of hydrogenase 1 oxygen tolerance is that all electrons required to directly reduce H(2) to water are readily available and can be rapidly transferred back to the active Ni-Fe site upon oxygen attack; this relies upon the unique properties of the proximal [4Fe-3S] cluster and the presence of a high potential medial [3Fe-4S] cluster [Lukey11, Roessler12, Evans13] (and see [Cracknell09, Parkin12]).

The physiological function of hydrogenase 1 is uncertain. Hydrogenase 1 and cytochrome bd-II terminal oxidase may constitute a respiratory chain between H(2) and oxygen that functions under microaerobic conditions [Atlung97, Wulff14]. Hydrogenase 1 may function in 'aerobic shock protection' - that is, it may function to keep intracellular O2 levels low (by reducing it to water), thus protecting O2-sensitive enzymes during the anaerobic to aerobic transition [Volbeda13].

The substrate specificity of hydrogenase 1 for various quinones is unknown [Laurinavichene01].

Maturation and membrane targeting of hydrogenase 1 involves proteins encoded within the hyp ( HypB, HypD, HypE), hya ( HyaD, HyaE, HyaF) and hyb operons ( HybF, HybG) and the HypF protein (reviewed in: [Bock06, Forzi07]).

Hydrogenase 1 content is increased during fermentation in the presence of formate [Sawers85]. Expression of the hya operon is induced under anaerobic conditions and in the presence of formate, but repressed by nitrate [Brondsted94, Richard99]. hya expresssion is repressed aerobically by the IscR repressor; AppY and ArcA prevent IscR repression under anaerobic conditions [Nesbit12]

E. coli K-12 contains a second respiratory hydrogenase - hydrogenase 2 - and a third hydrogenase - hydrogenase 3 - which is part of the formate hydrogenlyase complex. A potential fourth hydrogenase - hydrogenase 4 - is encoded within the hyf operon. Only hydrogenase 1 can reduce nitroblue tetrazolium in anaerobically growing E. coli cells [Pinske12b].

Review: [Vignais04]

Citations: [Pinske11, Ballantine85 , DerVartanian96, King99, Blokesch01, Hube02]

Locations: periplasmic space, inner membrane

Relationship Links: PDB:Structure:4GD3


GO Terms:
Biological Process:
Inferred from experimentGO:0006113 - fermentation [Sawers85]
Inferred from experimentGO:0009061 - anaerobic respiration [Ballantine85, Brondsted94]
Inferred from experimentGO:0019645 - anaerobic electron transport chain [Laurinavichene01]
Inferred from experimentGO:1902421 - hydrogen metabolic process [Sawers86, Laurinavichene01]
Molecular Function:
Inferred from experimentGO:0009055 - electron carrier activity [Volbeda12, Laurinavichene01]
Inferred from experimentGO:0016151 - nickel cation binding [Sawers86, DerVartanian96]
Inferred from experimentGO:0020037 - heme binding [Volbeda13]
Inferred from experimentGO:0033748 - hydrogenase (acceptor) activity [Sawers86, Lukey10, Menon91]
Inferred from experimentGO:0051536 - iron-sulfur cluster binding [Sawers86, DerVartanian96]
Cellular Component:
Inferred from experimentGO:0031237 - intrinsic component of periplasmic side of plasma membrane [Volbeda13]
Inferred by computational analysisInferred from experimentGO:0044569 - [Ni-Fe] hydrogenase complex [Volbeda13, DerVartanian96, Sawers86, Menon90]

Credits:
Created 23-Feb-2015 by Mackie A, Macquarie University


Enzymatic reaction of: hydrogen:menaquinone oxidoreductase (hydrogenase 1, oxygen tolerant hydrogenase)

Inferred from experiment

Synonyms: hydrogenlyase

EC Number: 1.12.99.6

Transport reaction diagram for hydrogen:menaquinone oxidoreductase

In Pathways: hydrogen to trimethylamine N-oxide electron transfer, hydrogen to dimethyl sulfoxide electron transfer

Summary:
The representation of the hydrogenase 1 complex depicts the transfer of electrons to menaquinone in the inner membrane. This representation has not been experimentally established and is therefore speculative.

Cofactors or Prosthetic Groups: a [4Fe-3S] iron-sulfur cluster [Volbeda12], a [3Fe-4S] iron-sulfur cluster [Volbeda12, DerVartanian96], a nickel-iron-sulfur cluster [Volbeda12, Sawers86, DerVartanian96], ferroheme b [Volbeda13], a [4Fe-4S] iron-sulfur cluster [Volbeda12, DerVartanian96]

Inhibitors (Unknown Mechanism): carbon monoxide [Sawers86], azide [Sawers86], N-bromosuccinimide [Sawers86]Kinetic Parameters:
Substrate Km (μM) Citations
H2 2.0 [Sawers86]

Enzymatic reaction of: hydrogen:oxygen oxidoreductase (hydrogenase 1, oxygen tolerant hydrogenase)

Inferred from experiment

EC Number: 1.12.99.6

2 H2 + oxygen → 2 H2O

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.


Sequence Features

Protein sequence of hydrogenase 1, b-type cytochrome subunit with features indicated

Feature Class Location Citations Comment
Pfam PF00033 12 -> 220
Inferred by computational analysis[Finn14]
Cytochrome_B : Cytochrome b/b6/petB
Transmembrane-Region 20 -> 40
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 64 -> 84
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 131 -> 151
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Metal-Binding-Site 181
Inferred from experiment[Volbeda13]
heme b ligand
Transmembrane-Region 186 -> 203
Inferred by computational analysis[UniProt15]
UniProt: Helical.


Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Unit

Transcription-unit diagram

Notes:

History:
10/20/97 Gene b0974 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10470; confirmed by SwissProt match.


References

Atlung97: Atlung T, Knudsen K, Heerfordt L, Brondsted L (1997). "Effects of sigmaS and the transcriptional activator AppY on induction of the Escherichia coli hya and cbdAB-appA operons in response to carbon and phosphate starvation." J Bacteriol 179(7);2141-6. PMID: 9079897

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Ballantine85: Ballantine SP, Boxer DH (1985). "Nickel-containing hydrogenase isoenzymes from anaerobically grown Escherichia coli K-12." J Bacteriol 163(2);454-9. PMID: 3894325

Blokesch01: Blokesch M, Magalon A, Bock A (2001). "Interplay between the specific chaperone-like proteins HybG and HypC in maturation of hydrogenases 1, 2, and 3 from Escherichia coli." J Bacteriol 183(9);2817-22. PMID: 11292801

Bock06: Bock A, King PW, Blokesch M, Posewitz MC (2006). "Maturation of hydrogenases." Adv Microb Physiol 51;1-71. PMID: 17091562

Brondsted94: Brondsted L, Atlung T (1994). "Anaerobic regulation of the hydrogenase 1 (hya) operon of Escherichia coli." J Bacteriol 176(17);5423-8. PMID: 8071220

Cracknell09: Cracknell JA, Wait AF, Lenz O, Friedrich B, Armstrong FA (2009). "A kinetic and thermodynamic understanding of O2 tolerance in [NiFe]-hydrogenases." Proc Natl Acad Sci U S A 106(49);20681-6. PMID: 19934053

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DerVartanian96: DerVartanian ME, Menon NK, Przybyla AE, Peck HD, DerVartanian DV (1996). "Electron paramagnetic resonance (EPR) studies on hydrogenase-1 (HYD1) purified from a mutant strain (AP6) of Escherichia coli enhanced in HYD1." Biochem Biophys Res Commun 227(1);211-5. PMID: 8858127

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Evans13: Evans RM, Parkin A, Roessler MM, Murphy BJ, Adamson H, Lukey MJ, Sargent F, Volbeda A, Fontecilla-Camps JC, Armstrong FA (2013). "Principles of sustained enzymatic hydrogen oxidation in the presence of oxygen--the crucial influence of high potential Fe-S clusters in the electron relay of [NiFe]-hydrogenases." J Am Chem Soc 135(7);2694-707. PMID: 23398301

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Forzi07: Forzi L, Sawers RG (2007). "Maturation of [NiFe]-hydrogenases in Escherichia coli." Biometals 20(3-4):565-78. PMID: 17216401

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hube02: Hube M, Blokesch M, Bock A (2002). "Network of hydrogenase maturation in Escherichia coli: role of accessory proteins HypA and HybF." J Bacteriol 184(14);3879-85. PMID: 12081959

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

King99: King PW, Przybyla AE (1999). "Response of hya expression to external pH in Escherichia coli." J Bacteriol 181(17);5250-6. PMID: 10464194

Laurinavichene01: Laurinavichene TV, Tsygankov AA (2001). "H2 consumption by Escherichia coli coupled via hydrogenase 1 or hydrogenase 2 to different terminal electron acceptors." FEMS Microbiol Lett 202(1);121-4. PMID: 11506918

Laurinavichene02: Laurinavichene TV, Zorin NA, Tsygankov AA (2002). "Effect of redox potential on activity of hydrogenase 1 and hydrogenase 2 in Escherichia coli." Arch Microbiol 178(6);437-42. PMID: 12420163

Lukey10: Lukey MJ, Parkin A, Roessler MM, Murphy BJ, Harmer J, Palmer T, Sargent F, Armstrong FA (2010). "How Escherichia coli is equipped to oxidize hydrogen under different redox conditions." J Biol Chem 285(6);3928-38. PMID: 19917611

Lukey11: Lukey MJ, Roessler MM, Parkin A, Evans RM, Davies RA, Lenz O, Friedrich B, Sargent F, Armstrong FA (2011). "Oxygen-tolerant [NiFe]-hydrogenases: the individual and collective importance of supernumerary cysteines at the proximal Fe-S cluster." J Am Chem Soc 133(42);16881-92. PMID: 21916508

Maciag11: Maciag A, Peano C, Pietrelli A, Egli T, De Bellis G, Landini P (2011). "In vitro transcription profiling of the {sigma}S subunit of bacterial RNA polymerase: re-definition of the {sigma}S regulon and identification of {sigma}S-specific promoter sequence elements." Nucleic Acids Res 39(13);5338-55. PMID: 21398637

Menon90: Menon NK, Robbins J, Peck HD, Chatelus CY, Choi ES, Przybyla AE (1990). "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames." J Bacteriol 1990;172(4);1969-77. PMID: 2180913

Menon91: Menon NK, Robbins J, Wendt JC, Shanmugam KT, Przybyla AE (1991). "Mutational analysis and characterization of the Escherichia coli hya operon, which encodes [NiFe] hydrogenase 1." J Bacteriol 173(15);4851-61. PMID: 1856178

Nesbit12: Nesbit AD, Fleischhacker AS, Teter SJ, Kiley PJ (2012). "ArcA and AppY antagonize IscR repression of hydrogenase-1 expression under anaerobic conditions, revealing a novel mode of O2 regulation of gene expression in Escherichia coli." J Bacteriol 194(24);6892-9. PMID: 23065979

Pandelia13: Pandelia ME, Bykov D, Izsak R, Infossi P, Giudici-Orticoni MT, Bill E, Neese F, Lubitz W (2013). "Electronic structure of the unique [4Fe-3S] cluster in O2-tolerant hydrogenases characterized by 57Fe Mossbauer and EPR spectroscopy." Proc Natl Acad Sci U S A 110(2);483-8. PMID: 23267108

Park06: Park YJ, Yoo CB, Choi SY, Lee HB (2006). "Purifications and characterizations of a ferredoxin and its related 2-oxoacid:ferredoxin oxidoreductase from the hyperthermophilic archaeon, Sulfolobus solfataricus P1." J Biochem Mol Biol 39(1);46-54. PMID: 16466637

Parkin12: Parkin A, Sargent F (2012). "The hows and whys of aerobic H2 metabolism." Curr Opin Chem Biol 16(1-2);26-34. PMID: 22366384

Pinske11: Pinske C, Sawers G (2011). "Iron restriction induces preferential down-regulation of H2-consuming over H2-evolving reactions during fermentative growth of Escherichia coli." BMC Microbiol 11;196. PMID: 21880124

Pinske12b: Pinske C, Jaroschinsky M, Sargent F, Sawers G (2012). "Zymographic differentiation of [NiFe]-hydrogenases 1, 2 and 3 of Escherichia coli K-12." BMC Microbiol 12;134. PMID: 22769583

Richard99: Richard DJ, Sawers G, Sargent F, McWalter L, Boxer DH (1999). "Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli." Microbiology 145 ( Pt 10);2903-12. PMID: 10537212

Roessler12: Roessler MM, Evans RM, Davies RA, Harmer J, Armstrong FA (2012). "EPR spectroscopic studies of the Fe-S clusters in the O2-tolerant [NiFe]-hydrogenase Hyd-1 from Escherichia coli and characterization of the unique [4Fe-3S] cluster by HYSCORE." J Am Chem Soc 134(37);15581-94. PMID: 22900997

Sawers85: Sawers RG, Ballantine SP, Boxer DH (1985). "Differential expression of hydrogenase isoenzymes in Escherichia coli K-12: evidence for a third isoenzyme." J Bacteriol 164(3);1324-31. PMID: 3905769

Sawers86: Sawers RG, Boxer DH (1986). "Purification and properties of membrane-bound hydrogenase isoenzyme 1 from anaerobically grown Escherichia coli K12." Eur J Biochem 1986;156(2);265-75. PMID: 3516689

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Vignais04: Vignais PM, Colbeau A (2004). "Molecular biology of microbial hydrogenases." Curr Issues Mol Biol 6(2);159-88. PMID: 15119826

Volbeda12: Volbeda A, Amara P, Darnault C, Mouesca JM, Parkin A, Roessler MM, Armstrong FA, Fontecilla-Camps JC (2012). "X-ray crystallographic and computational studies of the O2-tolerant [NiFe]-hydrogenase 1 from Escherichia coli." Proc Natl Acad Sci U S A 109(14);5305-10. PMID: 22431599

Volbeda13: Volbeda A, Darnault C, Parkin A, Sargent F, Armstrong FA, Fontecilla-Camps JC (2013). "Crystal structure of the O(2)-tolerant membrane-bound hydrogenase 1 from Escherichia coli in complex with its cognate cytochrome b." Structure 21(1);184-90. PMID: 23260654

Wulff14: Wulff P, Day CC, Sargent F, Armstrong FA (2014). "How oxygen reacts with oxygen-tolerant respiratory [NiFe]-hydrogenases." Proc Natl Acad Sci U S A 111(18);6606-11. PMID: 24715724

Other References Related to Gene Regulation

Bradley07: Bradley MD, Beach MB, de Koning AP, Pratt TS, Osuna R (2007). "Effects of Fis on Escherichia coli gene expression during different growth stages." Microbiology 153(Pt 9);2922-40. PMID: 17768236

Giel06: Giel JL, Rodionov D, Liu M, Blattner FR, Kiley PJ (2006). "IscR-dependent gene expression links iron-sulphur cluster assembly to the control of O-regulated genes in Escherichia coli." Mol Microbiol 60(4);1058-75. PMID: 16677314

Yamanaka14: Yamanaka Y, Oshima T, Ishihama A, Yamamoto K (2014). "Characterization of the YdeO regulon in Escherichia coli." PLoS One 9(11);e111962. PMID: 25375160


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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