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Escherichia coli K-12 substr. MG1655 Polypeptide: BglJ DNA-binding transcriptional regulator



Gene: bglJ Accession Numbers: G7948 (EcoCyc), b4366, ECK4356

Synonyms: yjjR

Regulation Summary Diagram: ?

Component of: RcsB-BglJ DNA-binding transcriptional activator (summary available)

Summary:
BglJ is a positive DNA-binding transcriptional regulator of transport and utilization of the aromatic β-glucosides arbutin and salicin [Giel96, Madhusudan05].

The expression of the gene bglJ induces the bglGFB operon via unknown mechanisms. This suggests the possibility that BglJ controls, directly or indirectly, the expression of the bgl operon [Giel96, Madhusudan05]. Currently, no experimental binding sites for this regulator have been reported in the literature.

BglJ is a protein that belongs to the LuxR/UhpA family of transcriptional regulators [Giel96]. It shows a potential helix-turn-helix motif in the carboxyl-terminal domain, which has 48% identity and 70% similarity to the corresponding domain of the RcsB protein [Madhusudan05].

Gene Citations: [Stratmann08]

Locations: cytosol

Map Position: [4,602,183 -> 4,602,860] (99.19 centisomes)
Length: 678 bp / 225 aa

Molecular Weight of Polypeptide: 25.622 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0014324 , DIP:DIP-9217N , EchoBASE:EB2479 , EcoGene:EG12594 , EcoliWiki:b4366 , Mint:MINT-1268192 , ModBase:P39404 , OU-Microarray:b4366 , PortEco:bglJ , PR:PRO_000022218 , Protein Model Portal:P39404 , RefSeq:NP_418786 , RegulonDB:G7948 , SMR:P39404 , String:511145.b4366 , Swiss-Model:P39404 , UniProt:P39404

Relationship Links: InterPro:IN-FAMILY:IPR000792 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR016032 , Pfam:IN-FAMILY:PF00196 , Prints:IN-FAMILY:PR00038 , Prosite:IN-FAMILY:PS00622 , Prosite:IN-FAMILY:PS50043 , Smart:IN-FAMILY:SM00421

In Paralogous Gene Group: 121 (40 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0045893 - positive regulation of transcription, DNA-templated Inferred from experiment Inferred by computational analysis [PerezRueda04, Giel96]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred from experiment Inferred by computational analysis [GOA01, PerezRueda04, Giel96]
GO:0005515 - protein binding Inferred from experiment [Venkatesh10]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11, GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred by curator
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
metabolism carbon utilization carbon compounds
regulation genetic unit regulated operon
regulation type of regulation transcriptional level activator

Essentiality data for bglJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 02-Apr-2008 by Santos-Zavaleta A , UNAM


Subunit of: RcsB-BglJ DNA-binding transcriptional activator

Synonyms: RcsBgl

Subunit composition of RcsB-BglJ DNA-binding transcriptional activator = [BglJ][RcsB]
         BglJ DNA-binding transcriptional regulator = BglJ (summary available)

Summary:
RcsB-BglJ is a pleiotropic transcriptional activator that coordinates regulation with global regulators, including CRP, LeuO, and HNS. BglJ-RcsB is active independently of RcsB phosphorylation [Venkatesh10, Stratmann12, Salscheider14]. Regulation by heteromeric transcription factors is a rare event in the bacterial word [Salscheider14]. A 20-bp consensus sequence motif has been proposed for RcsB-BglJ based on analysis with the MEME suite [Salscheider14].

RcsB-BglJ acts antagonistically as well as synergistically with additional transcription factors, such as HNS, CRP, and LeuO. Activation by RcsB-BglJ occurs both at non-HNS-repressed promoters as well as at promoters repressed by HNS and/or StpA [Salscheider14].

Credits:
Created 19-Jan-2011 by Peralta M , UNAM
Reviewed 02-Apr-2014 by Santos-Zavaleta A , UNAM

DNA binding site length: 20 base-pairs

Symmetry: Asymmetric

Consensus DNA Binding Sequence: c/aTTTATnnaTTCCtAAAATT

Regulated Transcription Units (13 total): ?

Notes:


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 146 -> 211
[UniProt11]
UniProt: HTH luxR-type.
DNA-Binding-Region 170 -> 189
[UniProt11]
UniProt: H-T-H motif; Non-Experimental Qualifier: by similarity.


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Ingrid Keseler on Mon Jun 20, 2011:
Gene start position corrected based on information in [Venkatesh10 ].
Peter D. Karp on Wed Jan 18, 2006:
Gene left-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Giel96: Giel M, Desnoyer M, Lopilato J (1996). "A mutation in a new gene, bglJ, activates the bgl operon in Escherichia coli K-12." Genetics 143(2);627-35. PMID: 8725214

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Madhusudan05: Madhusudan S, Paukner A, Klingen Y, Schnetz K (2005). "Independent regulation of H-NS-mediated silencing of the bgl operon at two levels: upstream by BglJ and LeuO and downstream by DnaKJ." Microbiology 151(Pt 10);3349-59. PMID: 16207917

PerezRueda04: Perez-Rueda E, Collado-Vides J, Segovia L (2004). "Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea." Comput Biol Chem 28(5-6);341-50. PMID: 15556475

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Salscheider14: Salscheider SL, Jahn A, Schnetz K (2014). "Transcriptional regulation by BglJ-RcsB, a pleiotropic heteromeric activator in Escherichia coli." Nucleic Acids Res 42(5);2999-3008. PMID: 24335284

Stratmann08: Stratmann T, Madhusudan S, Schnetz K (2008). "Regulation of the yjjQ-bglJ operon, encoding LuxR-type transcription factors, and the divergent yjjP gene by H-NS and LeuO." J Bacteriol 190(3);926-35. PMID: 18055596

Stratmann12: Stratmann T, Pul Ü, Wurm R, Wagner R, Schnetz K (2012). "RcsB-BglJ activates the Escherichia coli leuO gene, encoding an H-NS antagonist and pleiotropic regulator of virulence determinants." Mol Microbiol 83(6);1109-23. PMID: 22295907

UniProt11: UniProt Consortium (2011). "UniProt version 2011-11 released on 2011-11-22 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Venkatesh10: Venkatesh GR, Kembou Koungni FC, Paukner A, Stratmann T, Blissenbach B, Schnetz K (2010). "BglJ-RcsB heterodimers relieve repression of the Escherichia coli bgl operon by H-NS." J Bacteriol 192(24);6456-64. PMID: 20952573


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc13.