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Escherichia coli K-12 substr. MG1655 Polypeptide: putative transport protein, ABC superfamily - ATP-binding component

Gene: ftsE Accession Numbers: EG10340 (EcoCyc), b3463, ECK3447

Regulation Summary Diagram

Regulation summary diagram for ftsE

Component of: protein complex, putative transporter, ABC superfamily (summary available)

FtsE is the putative ATP-binding protein component of an ATP-binding cassette (ABC) transporter. FtsE dimerizes and associates with the inner membrane via interaction with FtsX, an integral membrane protein [deLeeuw99].

FtsE and FtsX localize to the cell division site; localization is dependent on FtsZ, FtsA and ZipA, but not FtsK, FtsQ, FtsL and FtsI [Schmidt04a]. FtsEX is important for assembly or stability of the septal ring under low-salt growth conditions [Schmidt04a].

An ftsE null mutant is only viable on high salt medium [deLeeuw99]. ftsE and ftsX are located in an operon with the ftsY gene. ftsY encodes a GTPase which acts as a receptor for the SRP (signal recognition particle) involved in protein targeting [Gill87, Miller94a]. It has been suggested that FtsE and FtsX are not to be required for SRP-mediated targeting [deLeeuw99], although it has been reported that ftsE mutants are affected in translocation of potassium ion pump proteins into the cytoplasmic membrane [Ukai98].

Gene Citations: [Gill90, Crickmore86, Gill86]

Locations: inner membrane, cytosol

Map Position: [3,600,102 <- 3,600,770] (77.59 centisomes, 279°)
Length: 669 bp / 222 aa

Molecular Weight of Polypeptide: 24.439 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0011313, CGSC:736, DIP:DIP-47843N, EchoBASE:EB0336, EcoGene:EG10340, EcoliWiki:b3463, Mint:MINT-1233007, ModBase:P0A9R7, OU-Microarray:b3463, PortEco:ftsE, PR:PRO_000022716, Protein Model Portal:P0A9R7, RefSeq:NP_417920, RegulonDB:EG10340, SMR:P0A9R7, String:511145.b3463, UniProt:P0A9R7

Relationship Links: InterPro:IN-FAMILY:IPR003439, InterPro:IN-FAMILY:IPR003593, InterPro:IN-FAMILY:IPR005286, InterPro:IN-FAMILY:IPR017871, InterPro:IN-FAMILY:IPR027417, Panther:IN-FAMILY:PTHR24220:SF186, Pfam:IN-FAMILY:PF00005, Prosite:IN-FAMILY:PS00211, Prosite:IN-FAMILY:PS50893, Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic

Gene-Reaction Schematic

GO Terms:
Biological Process:
Inferred from experimentGO:0046677 - response to antibiotic [Ricard73]
Inferred from experimentInferred by computational analysisGO:0051301 - cell division [UniProtGOA11a, GOA01a, Ukai98, Ricard73]
Inferred by computational analysisGO:0007049 - cell cycle [UniProtGOA11a]
Inferred by computational analysisGO:0008152 - metabolic process [GOA01a]
Molecular Function:
Inferred from experimentGO:0005515 - protein binding [deLeeuw99, Corbin07]
Inferred from experimentInferred by computational analysisGO:0005524 - ATP binding [UniProtGOA11a, GOA01a, deLeeuw99]
Inferred by computational analysisGO:0000166 - nucleotide binding [UniProtGOA11a]
Inferred by computational analysisGO:0016887 - ATPase activity [GOA01a]
Cellular Component:
Inferred from experimentGO:0005737 - cytoplasm [deLeeuw99]
Inferred from experimentGO:0019898 - extrinsic component of membrane [deLeeuw99]
Inferred from experimentGO:0031234 - extrinsic component of cytoplasmic side of plasma membrane [deLeeuw99]
Inferred from experimentGO:0032153 - cell division site [Schmidt04a]
Inferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a]

MultiFun Terms: cell processescell division
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily ATP binding cytoplasmic component

Essentiality data for ftsE knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB LennoxNo 37 Aerobic 7   No [Baba06, Comment 1]

Subunit of: protein complex, putative transporter, ABC superfamily

Inferred from experiment

Subunit composition of protein complex, putative transporter, ABC superfamily = [FtsE]2[FtsX]
         putative transport protein, ABC superfamily - ATP-binding component = FtsE (extended summary available)
         putative transport protein, ABC superfamily - membrane component = FtsX (summary available)

FtsE and FtsX are members of the ABC superfamily of transporters [Saurin99]. FtsE is the putative ATP-binding protein and FtsX is the putative membrane component. ftsE and ftsX were originally identified as conditional lethal mutants which failed to septate at the non-permissive temperature, implying a role in cell division [Taschner88]. FtsE and FtsX have been purified and shown to interact by co-immunoprecipitation [deLeeuw99]. The FtsEX complex may function as an ATP-dependent transporter which has a role in cell division and possibly salt transport.

Locations: inner membrane

GO Terms:
Cellular Component:
GO:0005886 - plasma membrane [deLeeuw99]

Sequence Features

Protein sequence of putative transport protein, ABC superfamily - ATP-binding component with features indicated

Feature Class Location Attached Group Citations Comment
Conserved-Region 2 -> 222  
Inferred by computational analysis[UniProt15]
UniProt: ABC transporter.
Pfam PF00005 18 -> 165  
Inferred by computational analysis[Finn14]
ABC_tran : ABC transporter
Nucleotide-Phosphate-Binding-Region 35 -> 42 ATP
Inferred by curator[deLeeuw99, UniProt15]
UniProt: ATP.
Mutagenesis-Variant 41  
Inferred from experiment[deLeeuw99]
UniProt: Does not bind ATP.
Mutagenesis-Variant 49  
Inferred from experiment[deLeeuw99]
UniProt: Prevents dimer formation. Does not alter ATP-binding.

Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Unit

Transcription-unit diagram


10/20/97 Gene b3463 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10340; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Corbin07: Corbin BD, Wang Y, Beuria TK, Margolin W (2007). "Interaction between cell division proteins FtsE and FtsZ." J Bacteriol 189(8);3026-35. PMID: 17307852

Crickmore86: Crickmore N, Salmond GP (1986). "The Escherichia coli heat shock regulatory gene is immediately downstream of a cell division operon: the fam mutation is allelic with rpoH." Mol Gen Genet 205(3);535-9. PMID: 3550388

deLeeuw99: de Leeuw E, Graham B, Phillips GJ, ten Hagen-Jongman CM, Oudega B, Luirink J (1999). "Molecular characterization of Escherichia coli FtsE and FtsX." Mol Microbiol 1999;31(3);983-93. PMID: 10048040

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gill86: Gill DR, Hatfull GF, Salmond GP (1986). "A new cell division operon in Escherichia coli." Mol Gen Genet 205(1);134-45. PMID: 3025556

Gill87: Gill DR, Salmond GP (1987). "The Escherichia coli cell division proteins FtsY, FtsE and FtsX are inner membrane-associated." Mol Gen Genet 1987;210(3);504-8. PMID: 3323846

Gill90: Gill DR, Salmond GP (1990). "The identification of the Escherichia coli ftsY gene product: an unusual protein." Mol Microbiol 1990;4(4);575-83. PMID: 2161989

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Miller94a: Miller JD, Bernstein HD, Walter P (1994). "Interaction of E. coli Ffh/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor." Nature 1994;367(6464);657-9. PMID: 8107852

Ricard73: Ricard M, Hirota Y (1973). "Process of cellular division in Escherichia coli: physiological study on thermosensitive mutants defective in cell division." J Bacteriol 116(1);314-22. PMID: 4583216

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

Schmidt04a: Schmidt KL, Peterson ND, Kustusch RJ, Wissel MC, Graham B, Phillips GJ, Weiss DS (2004). "A predicted ABC transporter, FtsEX, is needed for cell division in Escherichia coli." J Bacteriol 186(3);785-93. PMID: 14729705

Taschner88: Taschner PE, Huls PG, Pas E, Woldringh CL (1988). "Division behavior and shape changes in isogenic ftsZ, ftsQ, ftsA, pbpB, and ftsE cell division mutants of Escherichia coli during temperature shift experiments." J Bacteriol 1988;170(4);1533-40. PMID: 3280547

Ukai98: Ukai H, Matsuzawa H, Ito K, Yamada M, Nishimura A (1998). "ftsE(Ts) affects translocation of K+-pump proteins into the cytoplasmic membrane of Escherichia coli." J Bacteriol 1998;180(14);3663-70. PMID: 9658012

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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