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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted PTS permease - FrwD subunit

Gene: frwD Accession Numbers: EG11912 (EcoCyc), b3953, ECK3944

Synonyms: yijN, EIIB'frw, Enzyme IIB'frw

Regulation Summary Diagram

Regulation summary diagram for frwD

Component of: predicted PTS permease FrwCBD (summary available)

Sequence analysis indicates that an frwD encoded protein has similarity to the IIB domains of the PTS Enzymes II specific for fructose. frwD and frwB are similar to each other and to the IIB domains of the PTS Enzymes II specific for fructose. frwD contains conserved cysteine (cys10) and histidine residues (his16) predicted to be involved in phosphoryl transfer [Reizer95]

frwD is not expressed when cells are grown in rich medium [Wei01].

Locations: cytosol

Map Position: [4,145,161 -> 4,145,502] (89.34 centisomes, 322°)
Length: 342 bp / 113 aa

Molecular Weight of Polypeptide: 12.637 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012941, EchoBASE:EB1856, EcoGene:EG11912, EcoliWiki:b3953, OU-Microarray:b3953, PortEco:frwD, Pride:P32676, Protein Model Portal:P32676, RefSeq:NP_418388, RegulonDB:EG11912, SMR:P32676, String:511145.b3953, UniProt:P32676

Relationship Links: InterPro:IN-FAMILY:IPR003353, InterPro:IN-FAMILY:IPR003501, InterPro:IN-FAMILY:IPR013011, PDB:Structure:4TN5, Pfam:IN-FAMILY:PF02302, Prosite:IN-FAMILY:PS51099

In Paralogous Gene Group: 414 (2 members)

Gene-Reaction Schematic

Gene-Reaction Schematic

GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0008643 - carbohydrate transport [UniProtGOA11a]
Inferred by computational analysisGO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system [UniProtGOA11a, GOA01a, Reizer95]
Inferred by computational analysisGO:0015755 - fructose transport [GOA01a]
Inferred by computational analysisGO:0016310 - phosphorylation [UniProtGOA11a]
Inferred by computational analysisGO:0034219 - carbohydrate transmembrane transport [GOA01, GOA01a]
Molecular Function:
Inferred by computational analysisGO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity [GOA01, GOA01a]
Inferred by computational analysisGO:0016301 - kinase activity [UniProtGOA11a]
Inferred by computational analysisGO:0016740 - transferase activity [UniProtGOA11a]
Inferred by computational analysisGO:0022877 - protein-N(PI)-phosphohistidine-fructose phosphotransferase system transporter activity [GOA01a]
Cellular Component:
Inferred by computational analysisGO:0005737 - cytoplasm [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: metabolismcarbon utilizationcarbon compounds
transportGroup TranslocatorsPhosphotransferase Systems (PEP-dependent PTS)

Essentiality data for frwD knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: predicted PTS permease FrwCBD

Inferred by computational analysis

Synonyms: EIIFrw, Enzyme IIFrw

Subunit composition of predicted PTS permease FrwCBD = [FrwC][FrwB][FrwD][PtsA]
         predicted PTS permease - FrwC subunit = FrwC (summary available)
         predicted PTS permease - FrwB subunit = FrwB (summary available)
         predicted PTS permease - FrwD subunit = FrwD (summary available)
         fused predicted PTS enzyme : HPr component / enzyme I component / enzyme IIA component = PtsA (extended summary available)

FrwCBDPtsA is a putative PTS permease [Tchieu01] which belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its sugar substrates in a process called group translocation (reviewed by [Postma93]).

FrwC, FrwB, FrwD and PtsA (formerly FrwA) are all encoded within the frw gene cluster. The Frw proteins and protein domains are homologous to constituents of the fructose Enzyme II complexes. The frw gene cluster also encodes several enzymes concerned with anaerobic carbon metabolism [Reizer95]. FrwCBDPtsA is a member of the PTS Fructose-Mannitol (Fru) family of transporters [Saier14].

Please note that the presence of an frwCBDptsA encoded protein complex is hypothetical.

GO Terms:
Biological Process:
Inferred by computational analysisGO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system [Reizer95, Tchieu01]

Revised 30-Apr-2014 by Mackie A, Macquarie University

Sequence Features

Protein sequence of predicted PTS permease - FrwD subunit with features indicated

Feature Class Location Citations Comment
Conserved-Region 1 -> 100
Inferred by computational analysis[UniProt15]
UniProt: PTS EIIB type-2.
Pfam PF02302 6 -> 94
Inferred by computational analysis[Finn14]
PTS_IIB : PTS system, Lactose/Cellobiose specific IIB subunit
Active-Site 10
Inferred by computational analysis[UniProt10, Reizer95]
UniProt: Phosphocysteine intermediate; Non-Experimental Qualifier: by similarity;
Amino-Acid-Site 16
Inferred by computational analysis[Reizer95]
conserved residue

Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Unit

Transcription-unit diagram


10/20/97 Gene b3953 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11912; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Reizer95: Reizer J, Reizer A, Saier MH (1995). "Novel phosphotransferase system genes revealed by bacterial genome analysis--a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system." Microbiology 141 ( Pt 4);961-71. PMID: 7773398

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(1);D251-8. PMID: 24225317

Tchieu01: Tchieu JH, Norris V, Edwards JS, Saier MH (2001). "The complete phosphotransferase system in Escherichia coli." J Mol Microbiol Biotechnol 3(3);329-46. PMID: 11361063

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wei01: Wei Y, Lee JM, Richmond C, Blattner FR, Rafalski JA, LaRossa RA (2001). "High-density microarray-mediated gene expression profiling of Escherichia coli." J Bacteriol 183(2);545-56. PMID: 11133948

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Tue Jan 1, 2002, biocyc12.