Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Polypeptide: tonB energy transducing system - ExbD subunit



Gene: exbD Accession Numbers: EG10272 (EcoCyc), b3005, ECK2998

Regulation Summary Diagram: ?

Component of:
TonB energy transducing system (extended summary available)
ferric coprogen outer membrane transport complex
ferric coprogen transport system
ferric citrate outer membrane transport complex (summary available)
ferric dicitrate transport system
ferrichrome outer membrane transport complex
ferrichrome transport system
ferric enterobactin outer membrane transport complex
ferric enterobactin transport system (summary available)
vitamin B12 outer membrane transport complex
vitamin B12 transport system

Summary:
ExbB and ExbD proteins function as part of the TonB-dependent energy transduction system for the import of iron-siderophore complexes and vitamin B12 across the outer membrane. ExbB and ExbD are encoded by the exb operon in Escherichia coli [Held02].

Gene Citations: [EickHelmerich89, Ahmer95]

Locations: inner membrane

Map Position: [3,148,840 <- 3,149,265] (67.87 centisomes)
Length: 426 bp / 141 aa

Molecular Weight of Polypeptide: 15.527 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009866 , CGSC:33384 , DIP:DIP-47973N , EchoBASE:EB0268 , EcoGene:EG10272 , EcoliWiki:b3005 , OU-Microarray:b3005 , PortEco:exbD , PR:PRO_000022555 , Protein Model Portal:P0ABV2 , RefSeq:NP_417478 , RegulonDB:EG10272 , SMR:P0ABV2 , String:511145.b3005 , UniProt:P0ABV2

Relationship Links: InterPro:IN-FAMILY:IPR003400 , InterPro:IN-FAMILY:IPR014170 , PDB:Structure:2PFU , Pfam:IN-FAMILY:PF02472

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0050821 - protein stabilization Inferred from experiment [Ahmer95]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11]
GO:0043213 - bacteriocin transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Ollis12, Ollis12a, Ollis11]
GO:0031992 - energy transducer activity Inferred from experiment [Letain97]
GO:0042802 - identical protein binding Inferred from experiment [Rajagopala14, Ollis12, Ollis11]
GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Zhang07, Letain97]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell processes adaptations Fe aquisition
transport Electrochemical potential driven transporters Ion-gradient-driven energizers

Essentiality data for exbD knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: TonB energy transducing system

Subunit composition of TonB energy transducing system = [TonB][ExbB][ExbD]
         TonB energy transducing system - TonB subunit = TonB (extended summary available)
         TonB energy transducing system - ExbB subunit = ExbB (summary available)
         tonB energy transducing system - ExbD subunit = ExbD (summary available)

Component of:
ferric coprogen outer membrane transport complex
ferric citrate outer membrane transport complex (summary available)
ferrichrome outer membrane transport complex
ferric enterobactin outer membrane transport complex
vitamin B12 outer membrane transport complex

Summary:
TonB is a cytoplasmic membrane protein which transduces the proton motive force (pmf) of the cytoplasmic membrane to the outer membrane active transporters thus providing the energy source required for the import of iron-siderophore complexes and vitamin B12 across the outer membrane [Letain97]. The amino-terminal signal sequence of TonB is thought to span the cytoplasmic membrane, with the rest of the protein residing within the periplasmic space [Karlsson93]. TonB has been shown to come into close contact with proteins located in both membranes [Skare93]. Sucrose density gradient centrifugation studies found that TonB is distributed approximately equally in the inner and outer membrane fractions [Letain97]. In conjunction with cytoplasmic membrane proteins ExbB and ExbD, TonB forms an energy transduction complex which interacts with a variety of outer membrane active transporter proteins [Braun02]. When complexed with ExbB and ExbD, TonB is thought to adopt an energized conformation which is subsequently released from the cytoplasmic membrane to the outer membrane whereupon it interacts with an array of outer membrane proteins [Higgs02]. TonB is then thought to respond to the conformational changes induced in the active transport proteins upon substrate binding [Moeck97], releasing its stored energy to the active transporters and reassociating with ExbB and ExbD at the cytoplasmic membrane to be re-energized [Larsen03].

Locations: inner membrane, periplasmic space

GO Terms:

Biological Process: GO:0015682 - ferric iron transport Inferred from experiment [Gresock11]
GO:0015889 - cobalamin transport Inferred from experiment [Bassford76]
Molecular Function: GO:0031992 - energy transducer activity Inferred from experiment [Letain97]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment [Skare93]
GO:0042597 - periplasmic space Inferred from experiment [Postle88]


Subunit of: ferric coprogen outer membrane transport complex

Subunit composition of ferric coprogen outer membrane transport complex = [FhuE][(TonB)(ExbB)(ExbD)]
         ferric coprogen outer membrane porin FhuE = FhuE (summary available)
         TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                 TonB energy transducing system - TonB subunit = TonB (extended summary available)
                 TonB energy transducing system - ExbB subunit = ExbB (summary available)
                 tonB energy transducing system - ExbD subunit = ExbD (summary available)

Component of: ferric coprogen transport system

Locations: periplasmic space, outer membrane, inner membrane

GO Terms:

Cellular Component: GO:0005886 - plasma membrane
GO:0009279 - cell outer membrane
GO:0030288 - outer membrane-bounded periplasmic space

Credits:
Created 30-Oct-2011 by Mackie A , Macquarie University


Enzymatic reaction of: transport of ferric coprogen (ferric coprogen outer membrane transport complex)


Subunit of: ferric coprogen transport system

Subunit composition of ferric coprogen transport system = [(FhuC)2(FhuB)2(FhuD)][(FhuE)([TonB][ExbB][ExbD])]
         iron (III) hydroxamate ABC transporter = (FhuC)2(FhuB)2(FhuD) (extended summary available)
                 iron (III) hydroxamate ABC transporter - ATP binding subunit = FhuC
                 iron (III) hydroxamate ABC transporter - membrane subunit = FhuB
                 iron (III) hydroxamate ABC transporter - periplasmic binding protein = FhuD (summary available)
         ferric coprogen outer membrane transport complex = (FhuE)([TonB][ExbB][ExbD])
                 ferric coprogen outer membrane porin FhuE = FhuE (summary available)
                 TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                         TonB energy transducing system - TonB subunit = TonB (extended summary available)
                         TonB energy transducing system - ExbB subunit = ExbB (summary available)
                         tonB energy transducing system - ExbD subunit = ExbD (summary available)

Credits:
Created 30-Oct-2011 by Mackie A , Macquarie University


Enzymatic reaction of: transport of ferric coprogen (ferric coprogen transport system)


Subunit of: ferric citrate outer membrane transport complex

Subunit composition of ferric citrate outer membrane transport complex = [FecA][(TonB)(ExbB)(ExbD)]
         ferric citrate outer membrane porin FecA = FecA (summary available)
         TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                 TonB energy transducing system - TonB subunit = TonB (extended summary available)
                 TonB energy transducing system - ExbB subunit = ExbB (summary available)
                 tonB energy transducing system - ExbD subunit = ExbD (summary available)

Component of: ferric dicitrate transport system

Summary:
The outer membrane ferric citrate transport system is responsible for the transport of ferric citrate across the outer membrane by FecA energized by the TonB energy transducing system.

Locations: outer membrane, inner membrane, periplasmic space

GO Terms:

Cellular Component: GO:0005886 - plasma membrane
GO:0009279 - cell outer membrane
GO:0030288 - outer membrane-bounded periplasmic space


Enzymatic reaction of: transport of ferric citrate (ferric citrate outer membrane transport complex)


Subunit of: ferric dicitrate transport system

Subunit composition of ferric dicitrate transport system = [(FecE)2(FecC)(FecD)(FecB)][(FecA)([TonB][ExbB][ExbD])]
         ferric dicitrate ABC transporter = (FecE)2(FecC)(FecD)(FecB) (summary available)
                 ferric dicitrate ABC transporter - ATP binding subunit = FecE (summary available)
                 ferric dicitrate ABC transporter - membrane subunit = FecC (summary available)
                 ferric dicitrate ABC transporter - membrane subunit = FecD (summary available)
                 ferric dicitrate ABC transporter - periplasmic binding protein = FecB (summary available)
         ferric citrate outer membrane transport complex = (FecA)([TonB][ExbB][ExbD]) (summary available)
                 ferric citrate outer membrane porin FecA = FecA (summary available)
                 TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                         TonB energy transducing system - TonB subunit = TonB (extended summary available)
                         TonB energy transducing system - ExbB subunit = ExbB (summary available)
                         tonB energy transducing system - ExbD subunit = ExbD (summary available)

Locations: outer membrane, inner membrane, periplasmic space

GO Terms:

Cellular Component: GO:0005886 - plasma membrane
GO:0009279 - cell outer membrane
GO:0030288 - outer membrane-bounded periplasmic space


Enzymatic reaction of: transport of ferric dicitrate (ferric dicitrate transport system)


Subunit of: ferrichrome outer membrane transport complex

Subunit composition of ferrichrome outer membrane transport complex = [(TonB)(ExbB)(ExbD)][FhuA]
         TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                 TonB energy transducing system - TonB subunit = TonB (extended summary available)
                 TonB energy transducing system - ExbB subunit = ExbB (summary available)
                 tonB energy transducing system - ExbD subunit = ExbD (summary available)
         ferrichrome / phage / antibiotic outer membrane porin FhuA = FhuA (extended summary available)

Component of: ferrichrome transport system


Enzymatic reaction of: transport of ferrichrome (ferrichrome outer membrane transport complex)


Subunit of: ferrichrome transport system

Synonyms: iron (III) hydroxamate uptake system

Subunit composition of ferrichrome transport system = [(FhuC)2(FhuB)2(FhuD)][([TonB][ExbB][ExbD])(FhuA)]
         iron (III) hydroxamate ABC transporter = (FhuC)2(FhuB)2(FhuD) (extended summary available)
                 iron (III) hydroxamate ABC transporter - ATP binding subunit = FhuC
                 iron (III) hydroxamate ABC transporter - membrane subunit = FhuB
                 iron (III) hydroxamate ABC transporter - periplasmic binding protein = FhuD (summary available)
         ferrichrome outer membrane transport complex = ([TonB][ExbB][ExbD])(FhuA)
                 TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                         TonB energy transducing system - TonB subunit = TonB (extended summary available)
                         TonB energy transducing system - ExbB subunit = ExbB (summary available)
                         tonB energy transducing system - ExbD subunit = ExbD (summary available)
                 ferrichrome / phage / antibiotic outer membrane porin FhuA = FhuA (extended summary available)

Citations: [Wu95]


Enzymatic reaction of: transport of ferrichrome (ferrichrome transport system)


Subunit of: ferric enterobactin outer membrane transport complex

Subunit composition of ferric enterobactin outer membrane transport complex = [(TonB)(ExbB)(ExbD)][FepA]
         TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                 TonB energy transducing system - TonB subunit = TonB (extended summary available)
                 TonB energy transducing system - ExbB subunit = ExbB (summary available)
                 tonB energy transducing system - ExbD subunit = ExbD (summary available)
         ferric enterobactin / colicin B / colicin D outer membrane porin FepA = FepA (summary available)

Component of: ferric enterobactin transport system (summary available)


Enzymatic reaction of: transport of ferric enterobactin (ferric enterobactin outer membrane transport complex)


Subunit of: ferric enterobactin transport system

Subunit composition of ferric enterobactin transport system = [(FepC)2(FepD)(FepG)(FepB)][([TonB][ExbB][ExbD])(FepA)]
         ferric enterobactin ABC transporter = (FepC)2(FepD)(FepG)(FepB) (extended summary available)
                 ferric enterobactin ABC transporter - ATP binding subunit = FepC (extended summary available)
                 ferric enterobactin ABC transporter - membrane subunit = FepD
                 ferric enterobactin ABC transporter - membrane subunit = FepG
                 ferric enterobactin ABC transporter - periplasmic binding protein = FepB
         ferric enterobactin outer membrane transport complex = ([TonB][ExbB][ExbD])(FepA)
                 TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                         TonB energy transducing system - TonB subunit = TonB (extended summary available)
                         TonB energy transducing system - ExbB subunit = ExbB (summary available)
                         tonB energy transducing system - ExbD subunit = ExbD (summary available)
                 ferric enterobactin / colicin B / colicin D outer membrane porin FepA = FepA (summary available)

Summary:
FepA is a 22-stranded membrane-spanning beta barrel protein in the outer membrane. FepA is a TonB dependent active transporter that recognizes ferric enterobactin and translocates the molecule across the outer membrane into the periplasm. FepB is a periplasmic binding protein that binds ferric enterobactin for transport across the inner membrane by the FepCDG ABC transporter.

Locations: outer membrane, inner membrane, periplasmic space

GO Terms:

Cellular Component: GO:0005886 - plasma membrane
GO:0009279 - cell outer membrane
GO:0030288 - outer membrane-bounded periplasmic space


Enzymatic reaction of: ferric enterobactin transport


Subunit of: vitamin B12 outer membrane transport complex

Subunit composition of vitamin B12 outer membrane transport complex = [BtuB][(TonB)(ExbB)(ExbD)]
         vitamin B12 / E colicin / bacteriophage BF23 outer membrane porin BtuB = BtuB (extended summary available)
         TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                 TonB energy transducing system - TonB subunit = TonB (extended summary available)
                 TonB energy transducing system - ExbB subunit = ExbB (summary available)
                 tonB energy transducing system - ExbD subunit = ExbD (summary available)

Component of: vitamin B12 transport system

Locations: outer membrane, inner membrane, periplasmic space

GO Terms:

Cellular Component: GO:0005886 - plasma membrane
GO:0009279 - cell outer membrane
GO:0030288 - outer membrane-bounded periplasmic space


Enzymatic reaction of: transport of cobinamide (vitamin B12 outer membrane transport complex)


Enzymatic reaction of: transport of cob(I)alamin (vitamin B12 outer membrane transport complex)

Alternative Products for cob(I)alamin: coenzyme B12 [Bradbeer78 ]


Subunit of: vitamin B12 transport system

Subunit composition of vitamin B12 transport system = [(BtuB)([TonB][ExbB][ExbD])][(BtuD)2(BtuC)2(BtuF)]
         vitamin B12 outer membrane transport complex = (BtuB)([TonB][ExbB][ExbD])
                 vitamin B12 / E colicin / bacteriophage BF23 outer membrane porin BtuB = BtuB (extended summary available)
                 TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                         TonB energy transducing system - TonB subunit = TonB (extended summary available)
                         TonB energy transducing system - ExbB subunit = ExbB (summary available)
                         tonB energy transducing system - ExbD subunit = ExbD (summary available)
         vitamin B12 ABC transporter = (BtuD)2(BtuC)2(BtuF) (extended summary available)
                 vitamin B12 ABC transporter - ATP binding subunit = BtuD (summary available)
                 vitamin B12 ABC transporter - membrane subunit = BtuC (summary available)
                 vitamin B12 ABC transporter - periplasmic binding protein = BtuF (summary available)


Enzymatic reaction of: cobinamide transport (vitamin B12 transport system)


Enzymatic reaction of: cob(I)alamin transport (vitamin B12 transport system)

Alternative Products for cob(I)alamin: coenzyme B12 [Bradbeer78 ]


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 23 -> 43
[UniProt10a]
UniProt: Helical; Signal-anchor for type II membrane protein;; Non-Experimental Qualifier: probable;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b3005 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10272; confirmed by SwissProt match.


References

Ahmer95: Ahmer BM, Thomas MG, Larsen RA, Postle K (1995). "Characterization of the exbBD operon of Escherichia coli and the role of ExbB and ExbD in TonB function and stability." J Bacteriol 1995;177(16);4742-7. PMID: 7642501

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Barnard01: Barnard TJ, Watson ME, McIntosh MA (2001). "Mutations in the Escherichia coli receptor FepA reveal residues involved in ligand binding and transport." Mol Microbiol 2001;41(3);527-36. PMID: 11532122

Bassford76: Bassford PJ, Bradbeer C, Kadner RJ, Schnaitman CA (1976). "Transport of vitamin B12 in tonB mutants of Escherichia coli." J Bacteriol 128(1);242-7. PMID: 135755

Bradbeer78: Bradbeer C, Kenley JS, Di Masi DR, Leighton M (1978). "Transport of vitamin B12 in Escherichia coli. Corrinoid specificities of the periplasmic B12-binding protein and of energy-dependent B12 transport." J Biol Chem 253(5);1347-52. PMID: 342526

Braun02: Braun V, Braun M (2002). "Active transport of iron and siderophore antibiotics." Curr Opin Microbiol 5(2);194-201. PMID: 11934617

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

EickHelmerich89: Eick-Helmerich K, Braun V (1989). "Import of biopolymers into Escherichia coli: nucleotide sequences of the exbB and exbD genes are homologous to those of the tolQ and tolR genes, respectively." J Bacteriol 171(9);5117-26. PMID: 2670903

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Ferguson98: Ferguson AD, Hofmann E, Coulton JW, Diederichs K, Welte W (1998). "Siderophore-mediated iron transport: crystal structure of FhuA with bound lipopolysaccharide." Science 282(5397);2215-20. PMID: 9856937

Gaspar99: Gaspar M, Santos MA, Krauter K, Winkelmann G (1999). "Molecular recognition of synthetic siderophore analogues: a study with receptor-deficient and fhu(A-B) deletion mutants of Escherichia coli." Biometals 1999;12(3);209-18. PMID: 10581683

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Gresock11: Gresock MG, Savenkova MI, Larsen RA, Ollis AA, Postle K (2011). "Death of the TonB Shuttle Hypothesis." Front Microbiol 2;206. PMID: 22016747

Hantke75: Hantke K, Braun V (1975). "Membrane receptor dependent iron transport in Escherichia coli." FEBS Lett 49(3);301-5. PMID: 1089064

Hantke83: Hantke K (1983). "Identification of an iron uptake system specific for coprogen and rhodotorulic acid in Escherichia coli K12." Mol Gen Genet 191(2);301-6. PMID: 6353165

Held02: Held KG, Postle K (2002). "ExbB and ExbD do not function independently in TonB-dependent energy transduction." J Bacteriol 184(18);5170-3. PMID: 12193634

Higgs02: Higgs PI, Letain TE, Merriam KK, Burke NS, Park H, Kang C, Postle K (2002). "TonB interacts with nonreceptor proteins in the outer membrane of Escherichia coli." J Bacteriol 184(6);1640-8. PMID: 11872715

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Karlsson93: Karlsson M, Hannavy K, Higgins CF (1993). "A sequence-specific function for the N-terminal signal-like sequence of the TonB protein." Mol Microbiol 8(2);379-88. PMID: 8316087

Kenley78: Kenley JS, Leighton M, Bradbeer C (1978). "Transport of vitamin B12 in Escherichia coli. Corrinoid specificity of the outer membrane receptor." J Biol Chem 253(5);1341-6. PMID: 342525

Koster91: Koster W (1991). "Iron(III) hydroxamate transport across the cytoplasmic membrane of Escherichia coli." Biol Met 1991;4(1);23-32. PMID: 1830209

Larsen03: Larsen RA, Letain TE, Postle K (2003). "In vivo evidence of TonB shuttling between the cytoplasmic and outer membrane in Escherichia coli." Mol Microbiol 49(1);211-8. PMID: 12823822

Letain97: Letain TE, Postle K (1997). "TonB protein appears to transduce energy by shuttling between the cytoplasmic membrane and the outer membrane in Escherichia coli." Mol Microbiol 24(2);271-83. PMID: 9159515

Moeck97: Moeck GS, Coulton JW, Postle K (1997). "Cell envelope signaling in Escherichia coli. Ligand binding to the ferrichrome-iron receptor fhua promotes interaction with the energy-transducing protein TonB." J Biol Chem 272(45);28391-7. PMID: 9353297

Ollis11: Ollis AA, Postle K (2011). "The same periplasmic ExbD residues mediate in vivo interactions between ExbD homodimers and ExbD-TonB heterodimers." J Bacteriol 193(24);6852-63. PMID: 21984795

Ollis12: Ollis AA, Kumar A, Postle K (2012). "The ExbD periplasmic domain contains distinct functional regions for two stages in TonB energization." J Bacteriol 194(12);3069-77. PMID: 22493019

Ollis12a: Ollis AA, Postle K (2012). "Identification of functionally important TonB-ExbD periplasmic domain interactions in vivo." J Bacteriol 194(12);3078-87. PMID: 22493017

Postle88: Postle K, Skare JT (1988). "Escherichia coli TonB protein is exported from the cytoplasm without proteolytic cleavage of its amino terminus." J Biol Chem 263(22);11000-7. PMID: 2839513

Pressler88: Pressler U, Staudenmaier H, Zimmermann L, Braun V (1988). "Genetics of the iron dicitrate transport system of Escherichia coli." J Bacteriol 170(6);2716-24. PMID: 2836368

Pugsley76: Pugsley AP, Reeves P (1976). "Iron uptake in colicin B-resistant mutants of Escherichia coli K-12." J Bacteriol 126(3);1052-62. PMID: 7543

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

Roth96a: Roth JR, Lawrence JG, Bobik TA (1996). "Cobalamin (coenzyme B12): synthesis and biological significance." Annu Rev Microbiol 50;137-81. PMID: 8905078

Shea91: Shea CM, McIntosh MA (1991). "Nucleotide sequence and genetic organization of the ferric enterobactin transport system: homology to other periplasmic binding protein-dependent systems in Escherichia coli." Mol Microbiol 1991;5(6);1415-28. PMID: 1838574

Skare93: Skare JT, Ahmer BM, Seachord CL, Darveau RP, Postle K (1993). "Energy transduction between membranes. TonB, a cytoplasmic membrane protein, can be chemically cross-linked in vivo to the outer membrane receptor FepA." J Biol Chem 268(22);16302-8. PMID: 8344918

Sprencel00: Sprencel C, Cao Z, Qi Z, Scott DC, Montague MA, Ivanoff N, Xu J, Raymond KM, Newton SM, Klebba PE (2000). "Binding of ferric enterobactin by the Escherichia coli periplasmic protein FepB." J Bacteriol 182(19);5359-64. PMID: 10986237

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Yue03: Yue WW, Grizot S, Buchanan SK (2003). "Structural evidence for iron-free citrate and ferric citrate binding to the TonB-dependent outer membrane transporter FecA." J Mol Biol 332(2);353-68. PMID: 12948487

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

Zimmermann84: Zimmermann L, Hantke K, Braun V (1984). "Exogenous induction of the iron dicitrate transport system of Escherichia coli K-12." J Bacteriol 159(1);271-7. PMID: 6376472

Other References Related to Gene Regulation

Chen07: Chen Z, Lewis KA, Shultzaberger RK, Lyakhov IG, Zheng M, Doan B, Storz G, Schneider TD (2007). "Discovery of Fur binding site clusters in Escherichia coli by information theory models." Nucleic Acids Res 35(20);6762-77. PMID: 17921503

Newman99: Newman DL, Shapiro JA (1999). "Differential fiu-lacZ fusion regulation linked to Escherichia coli colony development." Mol Microbiol 33(1);18-32. PMID: 10411720


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Tue Nov 25, 2014, biocyc14.