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Escherichia coli K-12 substr. MG1655 Polypeptide: sulfate / thiosulfate ABC transporter - membrane subunit



Gene: cysW Accession Numbers: EG10198 (EcoCyc), b2423, ECK2418

Regulation Summary Diagram: ?

Component of:
sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp (extended summary available)
sulfate / thiosulfate / selenite / selenate ABC transporter CysAUWCysP (extended summary available)

Gene Citations: [Sirko90, Hryniewicz90]

Locations: inner membrane

Map Position: [2,538,826 <- 2,539,701] (54.72 centisomes)
Length: 876 bp / 291 aa

Molecular Weight of Polypeptide: 32.538 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007988 , CGSC:27371 , EchoBASE:EB0195 , EcoGene:EG10198 , EcoliWiki:b2423 , Mint:MINT-1250777 , OU-Microarray:b2423 , PortEco:cysW , PR:PRO_000022389 , Protein Model Portal:P0AEB0 , RefSeq:YP_026168 , RegulonDB:EG10198 , SMR:P0AEB0 , String:511145.b2423 , UniProt:P0AEB0

Relationship Links: InterPro:IN-FAMILY:IPR000515 , InterPro:IN-FAMILY:IPR005667 , InterPro:IN-FAMILY:IPR011866 , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

In Paralogous Gene Group: 24 (43 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0008272 - sulfate transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:1902358 - sulfate transmembrane transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0015116 - sulfate transmembrane transporter activity Inferred by computational analysis [GOA01a]
GO:0015419 - sulfate transmembrane-transporting ATPase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane
metabolism metabolism of other compounds sulfur metabolism
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Essentiality data for cysW knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]
Yes [Feist07, Comment 3]

Subunit of: sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp

Subunit composition of sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp = [Sbp][CysW][CysU][CysA]2
         sulfate / thiosulfate ABC transporter - periplasmic binding protein Sbp = Sbp
         sulfate / thiosulfate ABC transporter - membrane subunit = CysW
         sulfate / thiosulfate ABC transporter - membrane subunit = CysU
         sulfate / thiosulfate ABC transporter - ATP binding subunit = CysA

Summary:
CysAUWSbp and CysAUWCysP are sulfate transporters that belong to the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. Based on sequence similarity, CysA is the ATP-binding component; CysU and CysW are the membrane components, whilst CysP and Sbp are the periplasmic sulfate/thiosulfate binding components [Hryniewicz90, Sirko90, Sirko95].

Single cysP and spb insertional mutants are able to utilize both sulfate and thiosulfate as a sole sulfur source but both mutants have impaired growth compared with the wild-type strain [Sirko95]. The inactivation of both cysP and spb genes blocks the transport of both sulfate and thiosulfate, and the transport activity is restored by the presence of intact copies of either the cysP or sbp gene [Sirko95]. These results suggest that the two binding proteins have partially overlapping activities.

The CysAUWP-Sbp transport system acts as an alternate molybdate transport system although it is not clear whether it is used only in the absence of a functional molybdate specific system (ModABC) [Rosentel95, Self]. Additionally, kinetic analyses indicate that sulphate, selenite and selenate are all transported by the same system in E. coli K-12 [LindblowKull85]. In both of the aforementioned cases the role of the periplasmic binding proteins Sbp and CysP has not been elucidated.

Review: [AguilarBarajas11]

Citations: [Cervantes01]

Locations: inner membrane

GO Terms:

Cellular Component: GO:0043190 - ATP-binding cassette (ABC) transporter complex

Credits:
Last-Curated ? 19-Apr-2012 by Mackie A , Macquarie University


Enzymatic reaction of: sulfate ABC transporter (sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp)

EC Number: 3.6.3.25

Alternative Products for sulfate: chromate [Cervantes01 ]


Enzymatic reaction of: selenite ABC transporter

Synonyms: transport of selenite

Kinetic Parameters:

Substrate
Km (μM)
Citations
selenite
102.0
[LindblowKull85]


Enzymatic reaction of: selenate ABC transporter (sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp)

Synonyms: transport of selenate

Kinetic Parameters:

Substrate
Km (μM)
Citations
selenate
17.1
[LindblowKull85]


Enzymatic reaction of: thiosulphate ABC transporter (sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp)

Synonyms: transport of sulfate

EC Number: 3.6.3.25


Subunit of: sulfate / thiosulfate / selenite / selenate ABC transporter CysAUWCysP

Subunit composition of sulfate / thiosulfate / selenite / selenate ABC transporter CysAUWCysP = [CysA]2[CysW][CysU][CysP]
         sulfate / thiosulfate ABC transporter - ATP binding subunit = CysA
         sulfate / thiosulfate ABC transporter - membrane subunit = CysW
         sulfate / thiosulfate ABC transporter - membrane subunit = CysU
         sulfate / thiosulfate ABC transporter - periplasmic binding protein CysP = CysP

Summary:
CysAUWCysP and CysAUWSbp are sulfate transporters that belong to the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. Based on sequence similarity, CysA is the ATP-binding component; CysU and CysW are the membrane components, whilst CysP and Sbp are the periplasmic sulfate/thiosulfate binding components [Hryniewicz90, Sirko90, Sirko95].

Single cysP and spb insertional mutants are able to utilize both sulfate and thiosulfate as a sole sulfur source but both mutants have impaired growth compared with the wild-type strain [Sirko95]. The inactivation of both cysP and spb genes blocks the transport of both sulfate and thiosulfate, and the transport activity is restored by the presence of intact copies of either the cysP or sbp gene [Sirko95]. These results suggest that the two binding proteins have partially overlapping activities.

The CysAUWP-Sbp transport system acts as an alternate molybdate transport system although it is not clear whether it is used only in the absence of a functional molybdate specific system (ModABC) [Rosentel95, Self]. Additionally, kinetic analyses indicate that sulphate, selenite and selenate are all transported by the same system in E. coli K-12 [LindblowKull85]. In both the aforementioned cases the role of the periplasmic binding proteins Sbp and CysP has not been elucidated.

Review: [AguilarBarajas11]

Citations: [Cervantes01]

Credits:
Last-Curated ? 19-Apr-2012 by Mackie A , Macquarie University


Enzymatic reaction of: selenite ABC transporter (sulfate / thiosulfate / selenite / selenate ABC transporter CysAUWCysP)

Kinetic Parameters:

Substrate
Km (μM)
Citations
selenite
102.0
[LindblowKull85]


Enzymatic reaction of: selenate ABC transporter

Kinetic Parameters:

Substrate
Km (μM)
Citations
selenate
17.1
[LindblowKull85]


Enzymatic reaction of: sulfate ABC transporter (sulfate / thiosulfate / selenite / selenate ABC transporter CysAUWCysP)

Synonyms: transport of sulfate

EC Number: 3.6.3.25

Alternative Products for sulfate: chromate [Cervantes01 ]


Enzymatic reaction of: thiosulphate ABC transporter (sulfate / thiosulfate / selenite / selenate ABC transporter CysAUWCysP)

Synonyms: transport of thiosulfate

EC Number: 3.6.3.25


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 23 -> 43
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 66 -> 270
[UniProt09]
UniProt: ABC transmembrane type-1;
Transmembrane-Region 70 -> 90
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 105 -> 125
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 142 -> 162
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 201 -> 221
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 248 -> 268
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
10/20/97 Gene b2423 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10198; confirmed by SwissProt match.


References

AguilarBarajas11: Aguilar-Barajas E, Diaz-Perez C, Ramirez-Diaz MI, Riveros-Rosas H, Cervantes C (2011). "Bacterial transport of sulfate, molybdate, and related oxyanions." Biometals 24(4);687-707. PMID: 21301930

AlvarezSabin98: Alvarez-Sabin J (1998). "[Vertebrobasilar artery dissection]." Rev Neurol 26(149);148-53. PMID: 9533222

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Cervantes01: Cervantes C, Campos-Garcia J, Devars S, Gutierrez-Corona F, Loza-Tavera H, Torres-Guzman JC, Moreno-Sanchez R (2001). "Interactions of chromium with microorganisms and plants." FEMS Microbiol Rev 25(3);335-47. PMID: 11348688

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hryniewicz90: Hryniewicz M, Sirko A, Palucha A, Bock A, Hulanicka D (1990). "Sulfate and thiosulfate transport in Escherichia coli K-12: identification of a gene encoding a novel protein involved in thiosulfate binding." J Bacteriol 1990;172(6);3358-66. PMID: 2188959

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

LindblowKull85: Lindblow-Kull C, Kull FJ, Shrift A (1985). "Single transporter for sulfate, selenate, and selenite in Escherichia coli K-12." J Bacteriol 163(3);1267-9. PMID: 3897189

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Rosentel95: Rosentel JK, Healy F, Maupin-Furlow JA, Lee JH, Shanmugam KT (1995). "Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli." J Bacteriol 1995;177(17);4857-64. PMID: 7665461

Self: Self WT, Grunden AM, Hasona A, Shanmugam KT "Molybdate transport." Res Microbiol 152(3-4);311-21. PMID: 11421278

Sirko90: Sirko A, Hryniewicz M, Hulanicka D, Bock A (1990). "Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster." J Bacteriol 1990;172(6);3351-7. PMID: 2188958

Sirko95: Sirko A, Zatyka M, Sadowy E, Hulanicka D (1995). "Sulfate and thiosulfate transport in Escherichia coli K-12: evidence for a functional overlapping of sulfate- and thiosulfate-binding proteins." J Bacteriol 1995;177(14);4134-6. PMID: 7608089

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Other References Related to Gene Regulation

Grudniak11: Grudniak AM, Kurek A, Szarlak J, Wolska KI (2011). "Oleanolic and ursolic acids influence affect the expression of the cysteine regulon and the stress response in Escherichia coli." Curr Microbiol 62(4);1331-6. PMID: 21221969

Lochowska01: Lochowska A, Iwanicka-Nowicka R, Plochocka D, Hryniewicz MM (2001). "Functional dissection of the LysR-type CysB transcriptional regulator. Regions important for DNA binding, inducer response, oligomerization, and positive control." J Biol Chem 276(3);2098-107. PMID: 11038360

Lochowska04: Lochowska A, Iwanicka-Nowicka R, Zaim J, Witkowska-Zimny M, Bolewska K, Hryniewicz MM (2004). "Identification of activating region (AR) of Escherichia coli LysR-type transcription factor CysB and CysB contact site on RNA polymerase alpha subunit at the cysP promoter." Mol Microbiol 53(3);791-806. PMID: 15255893

Otsuka96: Otsuka J, Watanabe H, Mori KT (1996). "Evolution of transcriptional regulation system through promiscuous coupling of regulatory proteins with operons; suggestion from protein sequence similarities in Escherichia coli." J Theor Biol 1996;178(2);183-204. PMID: 8729576

WhiteZiegler07: White-Ziegler CA, Malhowski AJ, Young S (2007). "Human body temperature (37degrees C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12." J Bacteriol 189(15);5429-40. PMID: 17526711


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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