Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Pathway: BarA UvrY Two-Component Signal Transduction System

Diagram Key: ?

Locations of Mapped Genes:

Genetic Regulation Schematic: ?

Superclasses: Signal transduction pathways

Summary:
In E. coli, two-component regulatory systems sense and respond to changes in environmental conditions. In the prototypical system a membrane associated sensor kinase autophosphorylates in response to an environmental signal. The sensor kinase then transfers a phosphoryl group to a response regulator (RR) which, thus activated, effects a response. The majority of RRs in E.coli are transcription factors.

The BarA/UvrY two-component system comprises BarA - the membrane associated sensor kinase - and UvrY - the cytoplasmic response regulator. The BarA/UvrY two-component system is believed to play a role in central carbon metabolism via its regulation of the small non-coding RNAs CsrC and CsrB [Suzuki02, Weilbacher03, Liu97]. BarA is a tripartate sensor kinase which is predicted to phosphorylate its cognate response regulator UvrY via a His→Asp→His→Asp phosphorelay [Ishige94, Pernestig01]. Autophosphorylation of BarA has been demonstrated in vitro as has the transfer of phosphate from phosphorylated BarA to UvrY [Pernestig01, Yamamoto05]. The physiological signals that activate the BarA/UvrY system are not well characterised. Aliphatic carboxylic acids such as formate, acetate and propionate have been shown to be an environmental stimulus for the BarA sensor kinase [Chavez10].

Credits:
Created 29-Jul-2010 by Mackie A , Macquarie University


References

Chavez10: Chavez RG, Alvarez AF, Romeo T, Georgellis D (2010). "The physiological stimulus for the BarA sensor kinase." J Bacteriol 192(7);2009-12. PMID: 20118252

Ishige94: Ishige K, Nagasawa S, Tokishita S, Mizuno T (1994). "A novel device of bacterial signal transducers." EMBO J 1994;13(21);5195-202. PMID: 7957084

Liu97: Liu MY, Gui G, Wei B, Preston JF, Oakford L, Yuksel U, Giedroc DP, Romeo T (1997). "The RNA molecule CsrB binds to the global regulatory protein CsrA and antagonizes its activity in Escherichia coli." J Biol Chem 272(28);17502-10. PMID: 9211896

Pernestig01: Pernestig AK, Melefors O, Georgellis D (2001). "Identification of UvrY as the cognate response regulator for the BarA sensor kinase in Escherichia coli." J Biol Chem 2001;276(1);225-31. PMID: 11022030

Suzuki02: Suzuki K, Wang X, Weilbacher T, Pernestig AK, Melefors O, Georgellis D, Babitzke P, Romeo T (2002). "Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli." J Bacteriol 184(18);5130-40. PMID: 12193630

Weilbacher03: Weilbacher T, Suzuki K, Dubey AK, Wang X, Gudapaty S, Morozov I, Baker CS, Georgellis D, Babitzke P, Romeo T (2003). "A novel sRNA component of the carbon storage regulatory system of Escherichia coli." Mol Microbiol 48(3);657-70. PMID: 12694612

Yamamoto05: Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A (2005). "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." J Biol Chem 280(2);1448-56. PMID: 15522865

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Hirsch02: Hirsch M, Elliott T (2002). "Role of ppGpp in rpoS stationary-phase regulation in Escherichia coli." J Bacteriol 184(18);5077-87. PMID: 12193624

Mukhopadhyay00: Mukhopadhyay S, Audia JP, Roy RN, Schellhorn HE (2000). "Transcriptional induction of the conserved alternative sigma factor RpoS in Escherichia coli is dependent on BarA, a probable two-component regulator." Mol Microbiol 37(2);371-81. PMID: 10931332

Nagasawa92: Nagasawa S, Tokishita S, Aiba H, Mizuno T (1992). "A novel sensor-regulator protein that belongs to the homologous family of signal-transduction proteins involved in adaptive responses in Escherichia coli." Mol Microbiol 1992;6(6);799-807. PMID: 1574005

Pao95: Pao GM, Saier MH (1995). "Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution." J Mol Evol 1995;40(2);136-54. PMID: 7699720

Parkinson92: Parkinson JS, Kofoid EC (1992). "Communication modules in bacterial signaling proteins." Annu Rev Genet 1992;26;71-112. PMID: 1482126

Parkinson93: Parkinson JS (1993). "Signal transduction schemes of bacteria." Cell 1993;73(5);857-71. PMID: 8098993

Pernestig03: Pernestig AK, Georgellis D, Romeo T, Suzuki K, Tomenius H, Normark S, Melefors O (2003). "The Escherichia coli BarA-UvrY two-component system is needed for efficient switching between glycolytic and gluconeogenic carbon sources." J Bacteriol 185(3);843-53. PMID: 12533459

Sahu03: Sahu SN, Acharya S, Tuminaro H, Patel I, Dudley K, LeClerc JE, Cebula TA, Mukhopadhyay S (2003). "The bacterial adaptive response gene, barA, encodes a novel conserved histidine kinase regulatory switch for adaptation and modulation of metabolism in Escherichia coli." Mol Cell Biochem 253(1-2);167-77. PMID: 14619967

Stock90: Stock JB, Stock AM, Mottonen JM (1990). "Signal transduction in bacteria." Nature 1990;344(6265);395-400. PMID: 2157156

Tsuzuki95: Tsuzuki M, Ishige K, Mizuno T (1995). "Phosphotransfer circuitry of the putative multi-signal transducer, ArcB, of Escherichia coli: in vitro studies with mutants." Mol Microbiol 1995;18(5);953-62. PMID: 8825099

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC14B.