Locations of Mapped Genes:
|Superclasses:||Signal transduction pathways|
In E. coli, two-component regulatory systems sense and respond to changes in environmental conditions. In the prototypical system a membrane associated sensor kinase autophosphorylates in response to an environmental signal. The sensor kinase then transfers a phosphoryl group to a response regulator (RR) which, thus activated, effects a response. The majority of RRs in E.coli are transcription factors.
The BarA/UvrY two-component system comprises BarA - the membrane associated sensor kinase - and UvrY - the cytoplasmic response regulator. The BarA/UvrY two-component system is believed to play a role in central carbon metabolism via its regulation of the small non-coding RNAs CsrC and CsrB [Suzuki02, Weilbacher03, Liu97a]. BarA is a tripartate sensor kinase which is predicted to phosphorylate its cognate response regulator UvrY via a His→Asp→His→Asp phosphorelay [Ishige94, Pernestig01]. Autophosphorylation of BarA has been demonstrated in vitro as has the transfer of phosphate from phosphorylated BarA to UvrY [Pernestig01, Yamamoto05]. The physiological signals that activate the BarA/UvrY system are not well characterised. Aliphatic carboxylic acids such as formate, acetate and propionate have been shown to be an environmental stimulus for the BarA sensor kinase [Chavez10].
Liu97a: Liu MY, Gui G, Wei B, Preston JF, Oakford L, Yuksel U, Giedroc DP, Romeo T (1997). "The RNA molecule CsrB binds to the global regulatory protein CsrA and antagonizes its activity in Escherichia coli." J Biol Chem 272(28);17502-10. PMID: 9211896
Pernestig01: Pernestig AK, Melefors O, Georgellis D (2001). "Identification of UvrY as the cognate response regulator for the BarA sensor kinase in Escherichia coli." J Biol Chem 2001;276(1);225-31. PMID: 11022030
Suzuki02: Suzuki K, Wang X, Weilbacher T, Pernestig AK, Melefors O, Georgellis D, Babitzke P, Romeo T (2002). "Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli." J Bacteriol 184(18);5130-40. PMID: 12193630
Weilbacher03: Weilbacher T, Suzuki K, Dubey AK, Wang X, Gudapaty S, Morozov I, Baker CS, Georgellis D, Babitzke P, Romeo T (2003). "A novel sRNA component of the carbon storage regulatory system of Escherichia coli." Mol Microbiol 48(3);657-70. PMID: 12694612
Yamamoto05: Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A (2005). "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." J Biol Chem 280(2);1448-56. PMID: 15522865
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Mukhopadhyay00: Mukhopadhyay S, Audia JP, Roy RN, Schellhorn HE (2000). "Transcriptional induction of the conserved alternative sigma factor RpoS in Escherichia coli is dependent on BarA, a probable two-component regulator." Mol Microbiol 37(2);371-81. PMID: 10931332
Nagasawa92: Nagasawa S, Tokishita S, Aiba H, Mizuno T (1992). "A novel sensor-regulator protein that belongs to the homologous family of signal-transduction proteins involved in adaptive responses in Escherichia coli." Mol Microbiol 1992;6(6);799-807. PMID: 1574005
Pernestig03: Pernestig AK, Georgellis D, Romeo T, Suzuki K, Tomenius H, Normark S, Melefors O (2003). "The Escherichia coli BarA-UvrY two-component system is needed for efficient switching between glycolytic and gluconeogenic carbon sources." J Bacteriol 185(3);843-53. PMID: 12533459
Sahu03: Sahu SN, Acharya S, Tuminaro H, Patel I, Dudley K, LeClerc JE, Cebula TA, Mukhopadhyay S (2003). "The bacterial adaptive response gene, barA, encodes a novel conserved histidine kinase regulatory switch for adaptation and modulation of metabolism in Escherichia coli." Mol Cell Biochem 253(1-2);167-77. PMID: 14619967
Tsuzuki95: Tsuzuki M, Ishige K, Mizuno T (1995). "Phosphotransfer circuitry of the putative multi-signal transducer, ArcB, of Escherichia coli: in vitro studies with mutants." Mol Microbiol 1995;18(5);953-62. PMID: 8825099
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