Escherichia coli K-12 substr. MG1655 Pathway: BarA UvrY Two-Component Signal Transduction System
Inferred from experiment

Pathway diagram: BarA UvrY Two-Component Signal Transduction System

Diagram Key:

Locations of Mapped Genes:

Schematic showing all replicons, marked with selected genes

Genetic Regulation Schematic

Genetic regulation schematic for BarA UvrY Two-Component Signal Transduction System

Superclasses: Signal transduction pathways

In E. coli, two-component regulatory systems sense and respond to changes in environmental conditions. In the prototypical system a membrane associated sensor kinase autophosphorylates in response to an environmental signal. The sensor kinase then transfers a phosphoryl group to a response regulator (RR) which, thus activated, effects a response. The majority of RRs in E.coli are transcription factors.

The BarA/UvrY two-component system comprises BarA - the membrane associated sensor kinase - and UvrY - the cytoplasmic response regulator. The BarA/UvrY two-component system is believed to play a role in central carbon metabolism via its regulation of the small non-coding RNAs CsrC and CsrB [Suzuki02, Weilbacher03, Liu97]. BarA is a tripartate sensor kinase which is predicted to phosphorylate its cognate response regulator UvrY via a His→Asp→His→Asp phosphorelay [Ishige94, Pernestig01]. Autophosphorylation of BarA has been demonstrated in vitro as has the transfer of phosphate from phosphorylated BarA to UvrY [Pernestig01, Yamamoto05]. The physiological signals that activate the BarA/UvrY system are not well characterised. Aliphatic carboxylic acids such as formate, acetate and propionate have been shown to be an environmental stimulus for the BarA sensor kinase [Chavez10].

Citations: [Camacho15]

Created 29-Jul-2010 by Mackie A, Macquarie University


Camacho15: Camacho MI, Alvarez AF, Chavez RG, Romeo T, Merino E, Georgellis D (2015). "Effects of the global regulator CsrA on the BarA/UvrY two-component signaling system." J Bacteriol 197(5);983-91. PMID: 25535275

Chavez10: Chavez RG, Alvarez AF, Romeo T, Georgellis D (2010). "The physiological stimulus for the BarA sensor kinase." J Bacteriol 192(7);2009-12. PMID: 20118252

Ishige94: Ishige K, Nagasawa S, Tokishita S, Mizuno T (1994). "A novel device of bacterial signal transducers." EMBO J 1994;13(21);5195-202. PMID: 7957084

Liu97: Liu MY, Gui G, Wei B, Preston JF, Oakford L, Yuksel U, Giedroc DP, Romeo T (1997). "The RNA molecule CsrB binds to the global regulatory protein CsrA and antagonizes its activity in Escherichia coli." J Biol Chem 272(28);17502-10. PMID: 9211896

Pernestig01: Pernestig AK, Melefors O, Georgellis D (2001). "Identification of UvrY as the cognate response regulator for the BarA sensor kinase in Escherichia coli." J Biol Chem 2001;276(1);225-31. PMID: 11022030

Suzuki02: Suzuki K, Wang X, Weilbacher T, Pernestig AK, Melefors O, Georgellis D, Babitzke P, Romeo T (2002). "Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli." J Bacteriol 184(18);5130-40. PMID: 12193630

Weilbacher03: Weilbacher T, Suzuki K, Dubey AK, Wang X, Gudapaty S, Morozov I, Baker CS, Georgellis D, Babitzke P, Romeo T (2003). "A novel sRNA component of the carbon storage regulatory system of Escherichia coli." Mol Microbiol 48(3);657-70. PMID: 12694612

Yamamoto05: Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A (2005). "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." J Biol Chem 280(2);1448-56. PMID: 15522865

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hirsch02: Hirsch M, Elliott T (2002). "Role of ppGpp in rpoS stationary-phase regulation in Escherichia coli." J Bacteriol 184(18);5077-87. PMID: 12193624

KinoshitaKikuta16: Kinoshita-Kikuta E, Kinoshita E, Eguchi Y, Koike T (2016). "Validation of Cis and Trans Modes in Multistep Phosphotransfer Signaling of Bacterial Tripartite Sensor Kinases by Using Phos-Tag SDS-PAGE." PLoS One 11(2);e0148294. PMID: 26828204

Mukhopadhyay00a: Mukhopadhyay S, Audia JP, Roy RN, Schellhorn HE (2000). "Transcriptional induction of the conserved alternative sigma factor RpoS in Escherichia coli is dependent on BarA, a probable two-component regulator." Mol Microbiol 37(2);371-81. PMID: 10931332

Nagasawa92: Nagasawa S, Tokishita S, Aiba H, Mizuno T (1992). "A novel sensor-regulator protein that belongs to the homologous family of signal-transduction proteins involved in adaptive responses in Escherichia coli." Mol Microbiol 1992;6(6);799-807. PMID: 1574005

Pao95: Pao GM, Saier MH (1995). "Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution." J Mol Evol 1995;40(2);136-54. PMID: 7699720

Parkinson92: Parkinson JS, Kofoid EC (1992). "Communication modules in bacterial signaling proteins." Annu Rev Genet 1992;26;71-112. PMID: 1482126

Parkinson93: Parkinson JS (1993). "Signal transduction schemes of bacteria." Cell 1993;73(5);857-71. PMID: 8098993

Pernestig03: Pernestig AK, Georgellis D, Romeo T, Suzuki K, Tomenius H, Normark S, Melefors O (2003). "The Escherichia coli BarA-UvrY two-component system is needed for efficient switching between glycolytic and gluconeogenic carbon sources." J Bacteriol 185(3);843-53. PMID: 12533459

Sahu03: Sahu SN, Acharya S, Tuminaro H, Patel I, Dudley K, LeClerc JE, Cebula TA, Mukhopadhyay S (2003). "The bacterial adaptive response gene, barA, encodes a novel conserved histidine kinase regulatory switch for adaptation and modulation of metabolism in Escherichia coli." Mol Cell Biochem 253(1-2);167-77. PMID: 14619967

Stock90: Stock JB, Stock AM, Mottonen JM (1990). "Signal transduction in bacteria." Nature 1990;344(6265);395-400. PMID: 2157156

Tsuzuki95: Tsuzuki M, Ishige K, Mizuno T (1995). "Phosphotransfer circuitry of the putative multi-signal transducer, ArcB, of Escherichia coli: in vitro studies with mutants." Mol Microbiol 1995;18(5);953-62. PMID: 8825099

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zere15: Zere TR, Vakulskas CA, Leng Y, Pannuri A, Potts AH, Dias R, Tang D, Kolaczkowski B, Georgellis D, Ahmer BM, Romeo T (2015). "Genomic Targets and Features of BarA-UvrY (-SirA) Signal Transduction Systems." PLoS One 10(12);e0145035. PMID: 26673755

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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