Escherichia coli K-12 substr. MG1655 Pathway: ZraSR Two-Component Signal Transduction System
Inferred from experiment

Pathway diagram: ZraSR Two-Component Signal Transduction System

Diagram Key:

Locations of Mapped Genes:

Schematic showing all replicons, marked with selected genes

Genetic Regulation Schematic

Genetic regulation schematic for ZraSR Two-Component Signal Transduction System

Synonyms: HydHG Two Component Signal Transduction System

Superclasses: Signal transduction pathways

In E. coli, two-component regulatory systems sense and respond to changes in environmental conditions. In the prototypical system a membrane associated sensor kinase autophosphorylates in response to an environmental signal. The sensor kinase then transfers a phosphoryl group to a response regulator (RR) which, thus activated, effects a response. The majority of RRs in E. coli are transcription factors.

The ZraSR two-component system comprises ZraS - the membrane associated sensor kinase - and ZraR - the cytoplasmic response regulator. ZraSR regulates expression of zraP which encodes a periplasmic zinc-binding protein with a putative role in zinc homeostasis. Growth of E. coli K-12 in the presence of high external zinc or lead concentrations results in increased cellular levels of ZraR which are dependent on the presence of ZraS. Direct sensing of Zn2+ by ZraS has not been shown. [Leonhartsberger01]

At one time this system was known as the HydH/G system since it was thought to be involved in regulation of hydrogenase 3 activity [Stoker89].

Created 26-Jul-2010 by Mackie A, Macquarie University


Leonhartsberger01: Leonhartsberger S, Huber A, Lottspeich F, Bock A (2001). "The hydH/G Genes from Escherichia coli code for a zinc and lead responsive two-component regulatory system." J Mol Biol 2001;307(1);93-105. PMID: 11243806

Stoker89: Stoker K, Reijnders WN, Oltmann LF, Stouthamer AH (1989). "Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K-12." J Bacteriol 1989;171(8);4448-56. PMID: 2666400

Yamamoto05: Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A (2005). "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." J Biol Chem 280(2);1448-56. PMID: 15522865

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Bairoch93: Bairoch A, Boeckmann B (1993). "The SWISS-PROT protein sequence data bank, recent developments." Nucleic Acids Res. 21:3093-3096. PMID: 8332529

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Lee05b: Lee LJ, Barrett JA, Poole RK (2005). "Genome-wide transcriptional response of chemostat-cultured Escherichia coli to zinc." J Bacteriol 187(3);1124-34. PMID: 15659689

MendezOrtiz06: Mendez-Ortiz MM, Hyodo M, Hayakawa Y, Membrillo-Hernandez J (2006). "Genome-wide transcriptional profile of Escherichia coli in response to high levels of the second messenger 3',5'-cyclic diguanylic acid." J Biol Chem 281(12);8090-9. PMID: 16418169

Pao95: Pao GM, Saier MH (1995). "Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution." J Mol Evol 1995;40(2);136-54. PMID: 7699720

Parkinson92: Parkinson JS, Kofoid EC (1992). "Communication modules in bacterial signaling proteins." Annu Rev Genet 1992;26;71-112. PMID: 1482126

Parkinson93: Parkinson JS (1993). "Signal transduction schemes of bacteria." Cell 1993;73(5);857-71. PMID: 8098993

Sallai05: Sallai L, Tucker PA (2005). "Crystal structure of the central and C-terminal domain of the sigma(54)-activator ZraR." J Struct Biol 151(2);160-70. PMID: 16005641

Stock90: Stock JB, Stock AM, Mottonen JM (1990). "Signal transduction in bacteria." Nature 1990;344(6265);395-400. PMID: 2157156

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.5 on Mon Nov 30, 2015, BIOCYC13A.