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Escherichia coli K-12 substr. MG1655 Pathway: tRNA processing

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Locations of Mapped Genes:

Genetic Regulation Schematic: ?

Superclasses: Biosynthesis Nucleosides and Nucleotides Biosynthesis Nucleic Acid Processing

Summary:
tRNAs are the molecular adapters that allow the synthesis of proteins based on mRNAs transcribed from protein-coding genes. The active form of tRNA is generated by a combination of nuclease-based processing, the modification of select nucleosides, and finally charging via the tRNA charging.

tRNA processing is a multi-step pathway carried out by a mix of endonucleases and exonucleases. Processing begins with the endonucleolytic cleavage of individual tRNA precursors from RNAs containing multiple tRNAs. This initial cleavage can generate precursor molecules with a long 5' trailing end or with a short 5' extension. Precursors with a long trailer require additional exonucleolytic processing before the other nucleases in the pathway can act.

Following this initial processing step, a suite of ribonucleases cut down the 5' and 3' ends to yield the final, processed tRNA.

Although this processing pathway can function with as a few as one of its constituent exonucleases, the final tRNA product may be "imperfect," with a 3' end that may be longer or shorter than normal.

Credits:
Created 16-Jun-2010 by Shearer A , SRI International
Last-Curated ? 14-Jul-2010 by Shearer A , SRI International


References

Kelly92: Kelly KO, Deutscher MP (1992). "The presence of only one of five exoribonucleases is sufficient to support the growth of Escherichia coli." J Bacteriol 174(20);6682-4. PMID: 1400219

Li02: Li Z, Deutscher MP (2002). "RNase E plays an essential role in the maturation of Escherichia coli tRNA precursors." RNA 8(1);97-109. PMID: 11871663

Li94a: Li Z, Deutscher MP (1994). "The role of individual exoribonucleases in processing at the 3' end of Escherichia coli tRNA precursors." J Biol Chem 269(8);6064-71. PMID: 7509797

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Afonyushkin05: Afonyushkin T, Vecerek B, Moll I, Blasi U, Kaberdin VR (2005). "Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhB." Nucleic Acids Res 33(5);1678-89. PMID: 15781494

AitBara10: Ait-Bara S, Carpousis AJ (2010). "Characterization of the RNA degradosome of Pseudoalteromonas haloplanktis: conservation of the RNase E-RhlB interaction in the gammaproteobacteria." J Bacteriol 192(20);5413-23. PMID: 20729366

Alifano94: Alifano P, Rivellini F, Piscitelli C, Arraiano CM, Bruni CB, Carlomagno MS (1994). "Ribonuclease E provides substrates for ribonuclease P-dependent processing of a polycistronic mRNA." Genes Dev 8(24);3021-31. PMID: 8001821

Altman86: Altman S, Guerrier-Takada C (1986). "M1 RNA, the RNA subunit of Escherichia coli ribonuclease P, can undergo a pH-sensitive conformational change." Biochemistry 25(6);1205-8. PMID: 2421764

Altman95: Altman S (1995). "RNase P in research and therapy." Biotechnology (N Y) 13(4);327-9. PMID: 9634774

Amblar05: Amblar M, Arraiano CM (2005). "A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding." FEBS J 272(2);363-74. PMID: 15654875

Amblar06: Amblar M, Barbas A, Fialho AM, Arraiano CM (2006). "Characterization of the functional domains of Escherichia coli RNase II." J Mol Biol 360(5);921-33. PMID: 16806266

Andersen92: Andersen JT, Poulsen P, Jensen KF (1992). "Attenuation in the rph-pyrE operon of Escherichia coli and processing of the dicistronic mRNA." Eur J Biochem 206(2);381-90. PMID: 1375912

Ando01: Ando T, Tanaka T, Hori Y, Sakai E, Kikuchi Y (2001). "Human tyrosine tRNA is also internally cleavable by E. coli ribonuclease P RNA ribozyme in vitro." Biosci Biotechnol Biochem 65(12);2798-801. PMID: 11826982

Ando02: Ando T, Tanaka T, Hori Y, Kikuchi Y (2002). "Kinetics of hyperprocessing reaction of human tyrosine tRNA by ribonuclease P ribozyme from Escherichia coli." Biosci Biotechnol Biochem 66(9);1967-71. PMID: 12400701

Ando03: Ando T, Tanaka T, Kikuchi Y (2003). "Comparative analyses of hairpin substrate recognition by Escherichia coli and Bacillus subtilis ribonuclease P ribozymes." Biosci Biotechnol Biochem 67(8);1825-7. PMID: 12951523

Ando03a: Ando T, Tanaka T, Kikuchi Y (2003). "Substrate shape specificity of E coli RNase P ribozyme is dependent on the concentration of magnesium ion." J Biochem (Tokyo) 133(4);445-51. PMID: 12761291

Ando03b: Ando T, Tanaka T, Kikuchi Y (2003). "Bacterial ribonuclease P reaction is affected by substrate shape and magnesium ion concentration." Nucleic Acids Res Suppl (3);293-4. PMID: 14510496

Apirion78: Apirion D (1978). "Isolation, genetic mapping and some characterization of a mutation in Escherichia coli that affects the processing of ribonuleic acid." Genetics 90(4);659-71. PMID: 369943

Apirion80: Apirion D, Gitelman DR (1980). "Decay of RNA in RNA processing mutants of Escherichia coli." Mol Gen Genet 177(2);339-43. PMID: 6154228

Apirion80a: Apirion D (1980). "Genetic mapping and some characterization of the rnpA49 mutation of Escherichia coli that affects the RNA-processing enzyme ribonuclease P." Genetics 94(2);291-9. PMID: 6156104

Apirion93: Apirion D, Miczak A (1993). "RNA processing in prokaryotic cells." Bioessays 15(2);113-20. PMID: 7682412

Arraiano88: Arraiano CM, Yancey SD, Kushner SR (1988). "Stabilization of discrete mRNA breakdown products in ams pnp rnb multiple mutants of Escherichia coli K-12." J Bacteriol 170(10);4625-33. PMID: 2459106

Asha83: Asha PK, Blouin RT, Zaniewski R, Deutscher MP (1983). "Ribonuclease BN: identification and partial characterization of a new tRNA processing enzyme." Proc Natl Acad Sci U S A 80(11);3301-4. PMID: 6344080

Babitzke91: Babitzke P, Kushner SR (1991). "The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli." Proc Natl Acad Sci U S A 88(1);1-5. PMID: 1846032

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Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc14.