Escherichia coli K-12 substr. MG1655 Pathway: melibiose degradation
Inferred from experiment

Pathway diagram: melibiose degradation

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Locations of Mapped Genes:

Schematic showing all replicons, marked with selected genes

Genetic Regulation Schematic

Genetic regulation schematic for melibiose degradation

Superclasses: Degradation/Utilization/AssimilationCarbohydrates DegradationSugars Degradation

E. coli is able to utilize melibiose as a sole source of carbon. Melibiose is imported by the melibiose permease, MelB. The melibiose is then converted to β-D-glucose and α-D-galactose by the α-galactosidase encoded by melA.

Citations: [Burstein71, Pardee57]

Created 15-Feb-2007 by Johnson A, TIGR
Last-Curated 03-Mar-2011 by Keseler I, SRI International


Burstein71: Burstein C, Kepes A (1971). "The alpha-galactosidase from Escherichia coli K12." Biochim Biophys Acta 1971;230(1);52-63. PMID: 5543331

Pardee57: Pardee AB (1957). "An inducible mechanism for accumulation of melibiose in Escherichia coli." J Bacteriol 73(3);376-85. PMID: 13416199

Schmitt68: Schmitt R (1968). "Analysis of melibiose mutants deficient in alpha-galactosidase and thiomethylgalactoside permease II in Escherichia coli K-12." J Bacteriol 96(2);462-71. PMID: 4877127

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

BRENDA14: BRENDA team (2014). Imported from BRENDA version existing on Aug 2014.

Dey72: Dey PM, Pridham JB (1972). "Biochemistry of -galactosidases." Adv Enzymol Relat Areas Mol Biol 36;91-130. PMID: 4561015

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Hanatani84: Hanatani M, Yazyu H, Shiota-Niiya S, Moriyama Y, Kanazawa H, Futai M, Tsuchiya T (1984). "Physical and genetic characterization of the melibiose operon and identification of the gene products in Escherichia coli." J Biol Chem 1984;259(3);1807-12. PMID: 6319412

Legler86: Legler G, Pohl S (1986). "Synthesis of 5-amino-5-deoxy-D-galactopyranose and 1,5-dideoxy-1,5-imino-D-galactitol, and their inhibition of alpha- and beta-D-galactosidases." Carbohydr Res 155;119-29. PMID: 3024831

Liljestrom87: Liljestrom PL, Liljestrom P (1987). "Nucleotide sequence of the melA gene, coding for alpha-galactosidase in Escherichia coli K-12." Nucleic Acids Res 15(5);2213-20. PMID: 3031590

Mandrich02: Mandrich L, Caputo E, Martin BM, Rossi M, Manco G (2002). "The Aes protein and the monomeric alpha-galactosidase from Escherichia coli form a non-covalent complex. Implications for the regulation of carbohydrate metabolism." J Biol Chem 277(50);48241-7. PMID: 12374803

Nagao88: Nagao Y, Nakada T, Imoto M, Shimamoto T, Sakai S, Tsuda M, Tsuchiya T (1988). "Purification and analysis of the structure of alpha-galactosidase from Escherichia coli." Biochem Biophys Res Commun 1988;151(1);236-41. PMID: 2831880

Pourcher90: Pourcher T, Bassilana M, Sarkar HK, Kaback HR, Leblanc G (1990). "Melibiose permease and alpha-galactosidase of Escherichia coli: identification by selective labeling using a T7 RNA polymerase/promoter expression system." Biochemistry 29(3);690-6. PMID: 2159786

Schmitt66: Schmitt R, Rotman B (1966). "Alpha-galactosidase activity in cell-free extracts of Escherichia coli." Biochem Biophys Res Commun 22(5);473-9. PMID: 5329906

Shimamoto94a: Shimamoto T, Noguchi K, Kuroda M, Tsuda M, Tsuchiya T (1994). "Transcriptional attenuation and differential mRNA stability in the regulation of the Escherichia coli melibiose operon." J Biochem 115(6);1185-9. PMID: 7982902

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Thu Nov 26, 2015, biocyc14.