If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
Locations of Mapped Genes:
|Superclasses:||Degradation/Utilization/Assimilation → Carbohydrates Degradation → Sugars Degradation|
E. coli is able to utilize melibiose as a sole source of carbon. Melibiose is imported by the melibiose permease, MelB. The melibiose is then converted to β-D-glucose and α-D-galactose by the α-galactosidase encoded by melA.
Hanatani84: Hanatani M, Yazyu H, Shiota-Niiya S, Moriyama Y, Kanazawa H, Futai M, Tsuchiya T (1984). "Physical and genetic characterization of the melibiose operon and identification of the gene products in Escherichia coli." J Biol Chem 1984;259(3);1807-12. PMID: 6319412
Legler86: Legler G, Pohl S (1986). "Synthesis of 5-amino-5-deoxy-D-galactopyranose and 1,5-dideoxy-1,5-imino-D-galactitol, and their inhibition of alpha- and beta-D-galactosidases." Carbohydr Res 155;119-29. PMID: 3024831
Mandrich02: Mandrich L, Caputo E, Martin BM, Rossi M, Manco G (2002). "The Aes protein and the monomeric alpha-galactosidase from Escherichia coli form a non-covalent complex. Implications for the regulation of carbohydrate metabolism." J Biol Chem 277(50);48241-7. PMID: 12374803
Nagao88: Nagao Y, Nakada T, Imoto M, Shimamoto T, Sakai S, Tsuda M, Tsuchiya T (1988). "Purification and analysis of the structure of alpha-galactosidase from Escherichia coli." Biochem Biophys Res Commun 1988;151(1);236-41. PMID: 2831880
Pourcher90: Pourcher T, Bassilana M, Sarkar HK, Kaback HR, Leblanc G (1990). "Melibiose permease and alpha-galactosidase of Escherichia coli: identification by selective labeling using a T7 RNA polymerase/promoter expression system." Biochemistry 29(3);690-6. PMID: 2159786
Shimamoto94: Shimamoto T, Noguchi K, Kuroda M, Tsuda M, Tsuchiya T (1994). "Transcriptional attenuation and differential mRNA stability in the regulation of the Escherichia coli melibiose operon." J Biochem 115(6);1185-9. PMID: 7982902
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