If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
Locations of Mapped Genes:
|Superclasses:||Biosynthesis → Amino Acids Biosynthesis → Individual Amino Acids Biosynthesis → Alanine Biosynthesis|
Alanine is an essential component of protein (as L-alanine) and peptidoglycan (as a roughly 3:1 mix of D- and L-alanine). Only about a tenth of total alanine synthesized is incorporated into peptidoglycan.
At least three pathways (alanine biosynthesis I, alanine biosynthesis II, and alanine biosynthesis III) contribute to the synthesis of alanine. Alanine biosynthesis III is carried out in a single step by cysteine desulfurase, in a reaction that is critical for generation of iron-sulfur clusters, thiouridine groups in tRNAs, and a number of other sulfur- and selenium-containing proteins. Although this pathway does generate alanine, a major role in generating the cellular supply of alanine has not been demonstrated.
Superpathways: superpathway of alanine biosynthesis
Kambampati00: Kambampati R, Lauhon CT (2000). "Evidence for the transfer of sulfane sulfur from IscS to ThiI during the in vitro biosynthesis of 4-thiouridine in Escherichia coli tRNA." J Biol Chem 2000;275(15);10727-30. PMID: 10753862
Agar00: Agar JN, Krebs C, Frazzon J, Huynh BH, Dean DR, Johnson MK (2000). "IscU as a scaffold for iron-sulfur cluster biosynthesis: sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU." Biochemistry 39(27);7856-62. PMID: 10891064
Arifuzzaman06: Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H (2006). "Large-scale identification of protein-protein interaction of Escherichia coli K-12." Genome Res 16(5);686-91. PMID: 16606699
Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Ding04a: Ding H, Clark RJ, Ding B (2004). "IscA mediates iron delivery for assembly of iron-sulfur clusters in IscU under the limited accessible free iron conditions." J Biol Chem 279(36);37499-504. PMID: 15247288
Ding05: Ding H, Harrison K, Lu J (2005). "Thioredoxin reductase system mediates iron binding in IscA and iron delivery for the iron-sulfur cluster assembly in IscU." J Biol Chem 280(34);30432-7. PMID: 15985427
Flint96: Flint DH (1996). "Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase." J Biol Chem 1996;271(27);16068-74. PMID: 8663056
Forlani05: Forlani F, Cereda A, Freuer A, Nimtz M, Leimkuhler S, Pagani S (2005). "The cysteine-desulfurase IscS promotes the production of the rhodanese RhdA in the persulfurated form." FEBS Lett 579(30);6786-90. PMID: 16310786
Fujii00: Fujii T, Maeda M, Mihara H, Kurihara T, Esaki N, Hata Y (2000). "Structure of a NifS homologue: X-ray structure analysis of CsdB, an Escherichia coli counterpart of mammalian selenocysteine lyase." Biochemistry 2000;39(6);1263-73. PMID: 10684605
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