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Escherichia coli K-12 substr. MG1655 Enzyme: hydroxypyruvate isomerase



Gene: hyi Accession Numbers: G6277 (EcoCyc), b0508, ECK0501

Synonyms: ybbG, gip, glyoxylate-induced protein

Regulation Summary Diagram: ?

Subunit composition of hydroxypyruvate isomerase = [Hyi]2

Gene Citations: [Cusa99]

Locations: cytosol

Map Position: [534,934 -> 535,710] (11.53 centisomes)
Length: 777 bp / 258 aa

Molecular Weight of Polypeptide: 29.377 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001756 , DIP:DIP-9994N , EchoBASE:EB1543 , EcoGene:EG11584 , EcoliWiki:b0508 , ModBase:P30147 , OU-Microarray:b0508 , PortEco:hyi , PR:PRO_000022971 , Protein Model Portal:P30147 , RefSeq:NP_415041 , RegulonDB:G6277 , SMR:P30147 , String:511145.b0508 , Swiss-Model:P30147 , UniProt:P30147

Relationship Links: InterPro:IN-FAMILY:IPR013022 , InterPro:IN-FAMILY:IPR017643 , InterPro:IN-FAMILY:IPR026040 , Pfam:IN-FAMILY:PF01261

In Paralogous Gene Group: 145 (3 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0046487 - glyoxylate metabolic process Inferred from experiment [Ashiuchi99]
Molecular Function: GO:0008903 - hydroxypyruvate isomerase activity Inferred from experiment Inferred by computational analysis [GOA01, Ashiuchi99]
GO:0042803 - protein homodimerization activity Inferred from experiment [Ashiuchi99]
GO:0016853 - isomerase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism central intermediary metabolism unassigned reversible reactions

Essentiality data for hyi knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: hydroxypyruvate isomerase

Synonyms: hydroxypyruvate ketol-isomerase

EC Number: 5.3.1.22

hydroxypyruvate <=> tartronate semialdehyde

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Summary:
Hydroxypyruvate isomerase exclusively catalyzes the reversible isomerization between hydroxypyruvate and tartronate semialdehyde. [Ashiuchi99]


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Ashiuchi99: Ashiuchi M, Misono H (1999). "Biochemical evidence that Escherichia coli hyi (orf b0508, gip) gene encodes hydroxypyruvate isomerase." Biochim Biophys Acta 1999;1435(1-2);153-9. PMID: 10561547

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Cusa99: Cusa E, Obradors N, Baldoma L, Badia J, Aguilar J (1999). "Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli." J Bacteriol 1999;181(24);7479-84. PMID: 10601204

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Hasegawa08: Hasegawa A, Ogasawara H, Kori A, Teramoto J, Ishihama A (2008). "The transcription regulator AllR senses both allantoin and glyoxylate and controls a set of genes for degradation and reutilization of purines." Microbiology 154(Pt 11);3366-78. PMID: 18957590

Huerta03: Huerta AM, Collado-Vides J (2003). "Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals." J Mol Biol 333(2);261-78. PMID: 14529615

Rintoul02: Rintoul MR, Cusa E, Baldoma L, Badia J, Reitzer L, Aguilar J (2002). "Regulation of the Escherichia coli allantoin regulon: coordinated function of the repressor AllR and the activator AllS." J Mol Biol 324(4);599-610. PMID: 12460564

Teramoto10: Teramoto J, Yamanishi Y, Magdy el-SH, Hasegawa A, Kori A, Nakajima M, Arai F, Fukuda T, Ishihama A (2010). "Single live-bacterial cell assay of promoter activity and regulation." Genes Cells 15(11);1111-22. PMID: 20964794


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc12.