Escherichia coli K-12 substr. MG1655 Growth Medium: Davis and Mingioli medium A

This medium is used for cultivation of Escherichia coli.

Citations: [Atlas04]

Recipe Substances:
Substances Concentration Role
dipotassium phosphate 7 g/l Source of P
monopotassium phosphate 3 g/l Source of P
β-D-glucopyranose 2.5 g/l Source of C
ammonium sulfate 1 g/l Source of N S
sodium citrate trihydrate 0.5 g/l  
magnesium sulfate heptahydrate 100 mg/l Source of S
L-leucine 40 mg/l  
L-methionine 20 mg/l  
L-histidine 20 mg/l  
Constituents Concentration
K+ 101.8 mM
phosphate 61.841 mM
ammonium 15.136 mM
β-D-glucopyranose 13.877 mM
sulfate 7.9738 mM
citrate 2.0564 mM
Mg2+ 405.73 µM
L-leucine 304.94 µM
L-methionine 134.04 µM
L-histidine 128.9 µM

pH: 7.0

Osmolarity (approximate, computed from constituents): 0.2 Osm/L

Wildtype growth observations:

T (°C) O2 Growth?
37 Aerobic Yes


Atlas04: Atlas. Ronald M (2004). "Handbook of Microbiological Media."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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