M9 medium is a defined, minimal medium for E. coli. It is one of the simplest media in common use, providing a bare-bones complement of phosphorous, nitrogen, and sulfur. It is the medium of choice for microscopy of E. coli as it generates significantly less autofluorescence than other commonly used media such as LB [Xiao07].
This entry describes M9 medium with 1% glycerol as the carbon source. M9 with 1% glycerol has been used to evaluate conditionally essential genes [Joyce06].
| Recipe Substances:
Osmolarity (approximate, computed from constituents): 0.35 Osm/L
Wildtype growth observations:
113 single gene knockouts exhibit no growth (aerobic):
3705 single gene knockouts exhibit growth (aerobic):
Genes with inconsistent growth observations:
|Knockout||Growth?||T (°C)||O2||Growth Observations|
Yes [Steenbergen09, Comment 1]
Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394
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