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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted class II aldolase



Gene: ygbL Accession Numbers: G7419 (EcoCyc), b2738, ECK2733

Regulation Summary Diagram: ?

Summary:
No information about this protein was found by a literature search conducted on April 25, 2006.

Locations: cytosol

Map Position: [2,861,615 -> 2,862,253] (61.68 centisomes)
Length: 639 bp / 212 aa

Molecular Weight of Polypeptide: 23.222 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008994 , DIP:DIP-12113N , EchoBASE:EB2909 , EcoGene:EG13106 , EcoliWiki:b2738 , ModBase:Q46890 , OU-Microarray:b2738 , PortEco:ygbL , Pride:Q46890 , Protein Model Portal:Q46890 , RefSeq:NP_417218 , RegulonDB:G7419 , SMR:Q46890 , String:511145.b2738 , UniProt:Q46890

Relationship Links: InterPro:IN-FAMILY:IPR001303 , Pfam:IN-FAMILY:PF00596 , Smart:IN-FAMILY:SM01007

In Paralogous Gene Group: 450 (4 members)

GO Terms:

Molecular Function: GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism central intermediary metabolism unassigned reversible reactions

Essentiality data for ygbL knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Last-Curated ? 25-Apr-2006 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Metal-Binding-Site 79
[UniProt10]
UniProt: Zinc; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 98
[UniProt10]
UniProt: Zinc; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 100
[UniProt10]
UniProt: Zinc; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 165
[UniProt10]
UniProt: Zinc; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, BIOCYC13A.