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Escherichia coli K-12 substr. MG1655 Polypeptide: CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system



Gene: ypjF Accession Numbers: G7381 (EcoCyc), b2646, ECK2643

Regulation Summary Diagram: ?

Regulation summary diagram for ypjF

Summary:
Production of an artificially stabilized YpjF tagged protein causes inhibition of cell growth, compared to wild type [Brown03].

YeeV, YkfI, and YpjF have similarity to each other and define a protein family of cellular toxins [Brown03].

Locations: cytosol

Map Position: [2,775,475 -> 2,775,804] (59.82 centisomes, 215°)
Length: 330 bp / 109 aa

Molecular Weight of Polypeptide: 12.308 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008711 , EchoBASE:EB4032 , EcoGene:EG14284 , EcoliWiki:b2646 , OU-Microarray:b2646 , PortEco:ypjF , RefSeq:NP_417133 , RegulonDB:G7381 , String:511145.b2646 , UniProt:Q46953

Relationship Links: EcoO157Cyc:Homolog:Z3165 , EcoO157Cyc:Homolog:Z3165-MONOMER , InterPro:IN-FAMILY:IPR009610 , Pfam:IN-FAMILY:PF06755

In Paralogous Gene Group: 73 (2 members)

GO Terms:

Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: cell processes protection cell killing
extrachromosomal prophage genes and phage related functions

Essentiality data for ypjF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Brown03: Brown JM, Shaw KJ (2003). "A novel family of Escherichia coli toxin-antitoxin gene pairs." J Bacteriol 185(22);6600-8. PMID: 14594833

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Mon Apr 27, 2015, BIOCYC13A.