Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Polypeptide: CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system



Gene: ypjF Accession Numbers: G7381 (EcoCyc), b2646, ECK2643

Regulation Summary Diagram: ?

Summary:
Production of an artificially stabilized YpjF tagged protein causes inhibition of cell growth, compared to wild type [Brown03].

YeeV, YkfI, and YpjF have similarity to each other and define a protein family of cellular toxins [Brown03].

Locations: cytosol

Map Position: [2,775,475 -> 2,775,804] (59.82 centisomes)
Length: 330 bp / 109 aa

Molecular Weight of Polypeptide: 12.308 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008711 , EchoBASE:EB4032 , EcoGene:EG14284 , EcoliWiki:b2646 , OU-Microarray:b2646 , PortEco:ypjF , RefSeq:NP_417133 , RegulonDB:G7381 , String:511145.b2646 , UniProt:Q46953

Relationship Links: EcoO157Cyc:Homolog:Z3165 , EcoO157Cyc:Homolog:Z3165-MONOMER , InterPro:IN-FAMILY:IPR009610 , Pfam:IN-FAMILY:PF06755

In Paralogous Gene Group: 73 (2 members)

GO Terms:

Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: cell processes protection cell killing
extrachromosomal prophage genes and phage related functions

Essentiality data for ypjF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Brown03: Brown JM, Shaw KJ (2003). "A novel family of Escherichia coli toxin-antitoxin gene pairs." J Bacteriol 185(22);6600-8. PMID: 14594833

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC14A.