|Gene:||ratA||Accession Numbers: G7358 (EcoCyc), b2619, ECK2615|
RatA is a toxic protein that inhibits initiation of translation by specifically associating with the free 50S subunit of the ribosome and inhibiting 70S ribosome formation. RatA is unable to dissociate 70S ribosomes and has no effect on cellular mRNAs [Zhang11h].
Inducing expression of RatA causes inhibition of cell growth [Brown03a, Zhang11h]. Deletion of the orthologous pasTI locus alone in the uropathogenic E. coli CFT073 causes a defect in colonizing the kidneys of a mouse UTI model system and a defect in persister cell formation. In contrast, deletion of ratAB does not affect persister cell formation in E. coli K-12 MG1655 and does not increase sensitivity to nitrosative stress caused by acidified sodium nitrite (ASN). However, low-level expression of RatA does increase resistance to ASN [Norton12].
RatA: "ribosome association toxin A" [Zhang11h]
|Map Position: [2,752,310 <- 2,752,786] (59.32 centisomes, 214°)||Length: 477 bp / 158 aa|
Molecular Weight of Polypeptide: 17.727 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0008618 , EchoBASE:EB2985 , EcoGene:EG13193 , EcoliWiki:b2619 , OU-Microarray:b2619 , PortEco:yfjG , Protein Model Portal:P0AGL5 , RefSeq:NP_417109 , RegulonDB:G7358 , SMR:P0AGL5 , String:511145.b2619 , UniProt:P0AGL5
|Biological Process:||GO:0045947 - negative regulation of translational initiation [Zhang11h]|
|Molecular Function:||GO:0043023 - ribosomal large subunit binding [Zhang11h]|
|MultiFun Terms:||cell processes → protection → cell killing|
|Growth Medium||Growth?||T (°C)||O2||pH||Osm/L||Growth Observations|
|LB Lennox||Yes||37||Aerobic||7||Yes [Baba06, Comment 1]|
|M9 medium with 1% glycerol||Yes||37||Aerobic||7.2||0.35||Yes [Joyce06, Comment 2]|
|MOPS medium with 0.4% glucose||Yes||37||Aerobic||7.2||0.22||Yes [Baba06, Comment 1]|
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554
Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394
Norton12: Norton JP, Mulvey MA (2012). "Toxin-Antitoxin Systems Are Important for Niche-Specific Colonization and Stress Resistance of Uropathogenic Escherichia coli." PLoS Pathog 8(10);e1002954. PMID: 23055930
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