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Escherichia coli K-12 substr. MG1655 Protein: predicted structural protein, ethanolamine utilization microcompartment



Gene: eutM Accession Numbers: G7287 (EcoCyc), b2457, ECK2452

Synonyms: yffZ, cchA

Regulation Summary Diagram: ?

Subunit composition of predicted structural protein, ethanolamine utilization microcompartment = [EutM]6
         predicted structural protein, ethanolamine utilization microcompartment = EutM

Summary:
The EutM protein resembles carboxysomal shell proteins and can form a 2-dimensional protein membrane with an architecture that is similar to EutL. Unlike EutL, the pore structure of EutM appears positively charged [Takenoya10].

Crystal structures of EutM has been determined at 2.1 Å [Tanaka10], 2.7 Å and 2.0 Å [Takenoya10] resolution. The α/β structure resembles that of carboxysomal shell proteins. The flat, cyclic hexameric structure with a central pore may be a basic building block for a microcompartment shell [Tanaka10, Takenoya10].

The functions of the orthologous eut operon genes for ethanolamine utilization in Salmonella typhimurium have been studied experimentally [Kofoid99, Penrod06].

Comment: [Kang10a]

Locations: cytosol

Map Position: [2,570,179 <- 2,570,472] (55.4 centisomes)
Length: 294 bp / 97 aa

Molecular Weight of Polypeptide: 9.866 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008095 , EchoBASE:EB3939 , EcoGene:EG14187 , EcoliWiki:b2457 , OU-Microarray:b2457 , PortEco:eutM , PR:PRO_000022545 , Protein Model Portal:P0ABF4 , RefSeq:NP_416952 , RegulonDB:G7287 , SMR:P0ABF4 , String:511145.b2457 , Swiss-Model:P0ABF4 , UniProt:P0ABF4

Relationship Links: InterPro:IN-FAMILY:IPR000249 , InterPro:IN-FAMILY:IPR020808 , PDB:Structure:3I6P , PDB:Structure:3MPW , PDB:Structure:3MPY , Pfam:IN-FAMILY:PF00936 , Prosite:IN-FAMILY:PS01139 , Smart:IN-FAMILY:SM00877

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0046336 - ethanolamine catabolic process Inferred by computational analysis [UniProtGOA12]
Molecular Function: GO:0005198 - structural molecule activity Inferred by computational analysis [Tanaka10]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]
GO:0031471 - ethanolamine degradation polyhedral organelle Inferred by computational analysis [Tanaka10]

MultiFun Terms: cell structure

Essentiality data for eutM knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Created 07-Jan-2010 by Keseler I , SRI International
Last-Curated ? 29-Sep-2010 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 25
[UniProt10]
UniProt: Non-Experimental Qualifier: potential;
Chain 26 -> 97
[UniProt09]
UniProt: Ethanolamine utilization protein eutM;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kang10a: Kang S, Douglas T (2010). "Biochemistry. Some enzymes just need a space of their own." Science 327(5961);42-3. PMID: 20044564

Kofoid99: Kofoid E, Rappleye C, Stojiljkovic I, Roth J (1999). "The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins." J Bacteriol 181(17);5317-29. PMID: 10464203

Penrod06: Penrod JT, Roth JR (2006). "Conserving a volatile metabolite: a role for carboxysome-like organelles in Salmonella enterica." J Bacteriol 188(8);2865-74. PMID: 16585748

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Takenoya10: Takenoya M, Nikolakakis K, Sagermann M (2010). "Crystallographic insights into the pore structures and mechanisms of the EutL and EutM shell proteins of the Eut-BMC." J Bacteriol. PMID: 20851901

Tanaka10: Tanaka S, Sawaya MR, Yeates TO (2010). "Structure and mechanisms of a protein-based organelle in Escherichia coli." Science 327(5961);81-4. PMID: 20044574

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Other References Related to Gene Regulation

Oberto10: Oberto J (2010). "FITBAR: a web tool for the robust prediction of prokaryotic regulons." BMC Bioinformatics 11;554. PMID: 21070640


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14B.