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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted outer membrane porin protein



Gene: yfaZ Accession Numbers: G7163 (EcoCyc), b2250, ECK2243

Regulation Summary Diagram: ?

Summary:
YfaZ localizes to the outer membrane [Marani06].

Locations: outer membrane

Map Position: [2,361,755 <- 2,362,297] (50.9 centisomes)
Length: 543 bp / 180 aa

Molecular Weight of Polypeptide: 18.61 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007445 , EchoBASE:EB3841 , EcoGene:EG14088 , EcoliWiki:b2250 , OU-Microarray:b2250 , PortEco:yfaZ , Protein Model Portal:P76471 , RefSeq:NP_416753 , RegulonDB:G7163 , SMR:P76471 , String:511145.b2250 , UniProt:P76471

Relationship Links: InterPro:IN-FAMILY:IPR009998 , InterPro:IN-FAMILY:IPR011250 , InterPro:IN-FAMILY:IPR023614 , Pfam:IN-FAMILY:PF07437

GO Terms:

Cellular Component: GO:0009279 - cell outer membrane Inferred from experiment Inferred by computational analysis [GOA01a, Marani06]
GO:0016021 - integral component of membrane Inferred by computational analysis [GOA01a]

Gene Class: UNCLASSIFIED

Essentiality data for yfaZ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 03-Jul-2014 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 21
[UniProt10a]
UniProt: Non-Experimental Qualifier: potential;
Chain 22 -> 180
[UniProt09]
UniProt: Uncharacterized protein yfaZ;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Marani06: Marani P, Wagner S, Baars L, Genevaux P, de Gier JW, Nilsson I, Casadio R, von Heijne G (2006). "New Escherichia coli outer membrane proteins identified through prediction and experimental verification." Protein Sci 15(4);884-9. PMID: 16522795

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC14B.