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Escherichia coli K-12 substr. MG1655 Polypeptide: YeeO MATE transporter



Gene: yeeO Accession Numbers: G7070 (EcoCyc), b1985, ECK1980

Regulation Summary Diagram: ?

Summary:
The YeeO protein is an uncharacterised member of the MATE family of multidrug efflux transporters [Brown99]. Based on sequence similarity, YeeO may function as a proton-driven efflux system.

Locations: inner membrane

Map Position: [2,056,227 <- 2,057,870] (44.32 centisomes)
Length: 1644 bp / 547 aa

Molecular Weight of Polypeptide: 60.061 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0006586 , EchoBASE:EB3164 , EcoGene:EG13383 , EcoliWiki:b1985 , OU-Microarray:b1985 , PortEco:yeeO , Protein Model Portal:P76352 , RefSeq:NP_416491 , RegulonDB:G7070 , SMR:P76352 , String:511145.b1985 , UniProt:P76352

Relationship Links: InterPro:IN-FAMILY:IPR002528 , Pfam:IN-FAMILY:PF01554

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006855 - drug transmembrane transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0015238 - drug transmembrane transporter activity Inferred by computational analysis [GOA01a]
GO:0015297 - antiporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure membrane
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for yeeO knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Brown99: Brown MH, Paulsen IT, Skurray RA (1999). "The multidrug efflux protein NorM is a prototype of a new family of transporters." Mol Microbiol 1999;31(1);394-5. PMID: 9987140

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14A.