Escherichia coli K-12 substr. MG1655 Polypeptide: spermidine efflux transporter - MdtJ subunit

Gene: mdtJ Accession Numbers: G6858 (EcoCyc), b1600, ECK1595

Synonyms: ydgF

Regulation Summary Diagram: ?

Regulation summary diagram for mdtJ

Component of: spermidine efflux transporter (extended summary available)

MdtJ is part of the MdtJI spermidine SMR transporter.

Locations: inner membrane

Map Position: [1,671,160 <- 1,671,525] (36.02 centisomes, 130°)
Length: 366 bp / 121 aa

Molecular Weight of Polypeptide: 13.115 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0005345 , EchoBASE:EB3686 , EcoGene:EG13927 , EcoliWiki:b1600 , ModBase:P69212 , OU-Microarray:b1600 , PortEco:mdtJ , PR:PRO_000023190 , Protein Model Portal:P69212 , RefSeq:NP_416117 , RegulonDB:G6858 , String:511145.b1600 , UniProt:P69212

Relationship Links: InterPro:IN-FAMILY:IPR000390 , InterPro:IN-FAMILY:IPR023740 , Panther:IN-FAMILY:PTHR30561:SF2 , Pfam:IN-FAMILY:PF00893

In Paralogous Gene Group: 316 (2 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0015848 - spermidine transport Inferred from experiment [Higashi08]
GO:1903711 - spermidine transmembrane transport Inferred by computational analysis Inferred from experiment [Higashi08, GOA06, GOA01]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0015606 - spermidine transmembrane transporter activity Inferred from experiment Inferred by computational analysis [GOA06, GOA01, Higashi08]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA06, GOA01, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: cell processes protection cell killing
cell structure membrane
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for mdtJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: spermidine efflux transporter

Synonyms: MdtJI spermidine SMR transporter

Subunit composition of spermidine efflux transporter = [MdtJ][MdtI]
         spermidine efflux transporter - MdtJ subunit = MdtJ (summary available)
         spermidine efflux transporter - MdtI subunit = MdtI (summary available)

MdtJ and MdtI are two components of a spermidine exporter. Based on homology and hydropathy analysis, the products of mdtJI are considered two-component-type, Small Multidrug Resistance (SMR) family, proton-dependent, transporter proteins [Paulsen96a, Jack00a].

Sequence analysis and gene fusion studies indicate that MdtJ and MdtI are homologous, oppositely oriented proteins which form an antiparallel heterodimer or higher oligomer within the inner membrane [Rapp06]. Protein topology studies of MdtI in the inner membrane reveals a structure with 4 transmembrane segments and both the N- and C-terminus in the periplasm [Drew02]. Important residues involved in spermidine export have been identified by mutation analysis [Higashi08].

Episomal expression of mdtJI resulted in excretion of spermidine in transport assays and allowed recovery of cells from spermidine toxicity due to over-accumulation [Higashi08]. Drug-resistance studies indicate that overexpression of mdtJI in an expression vector conferred increased resistance to deoxycholate (4-fold), nalidixic acid (2-fold), fosfomycin (2-fold), and SDS ( 2-fold) [Nishino01]. Episomal expression of mdtJ causes inhibition of cell growth, compared to wild type [Brown03].

mdtJI expression increased during growth in spermidine [Higashi08]. Expression of mdtJalso increased very slightly due to amplification of evgA, baeR, cpxR, ompR, and rcsB [Nishino02, Hirakawa03a], increased upon exposure of wild-type E. coli strain CF1943 (W3110) to puromycin [Sabina03], increased during growth in L-idonate [Bausch04], increase during growth at pH 5.7 or 7.0 compared to 8.5 [Hayes06], and was found to be growth-phase independent in complex media [Kobayashi06a].

Citations: [Hirakawa06]

Last-Curated ? 11-Jan-2008 by Johnson A , JCVI

Enzymatic reaction of: Transport of spermidine (spermidine efflux transporter)

Transport reaction diagram for Transport of spermidine

Sequence Features

Protein sequence of spermidine efflux transporter - MdtJ subunit with features indicated

Feature Class Location Common Name Citations Comment
Transmembrane-Region 1 -> 23 MdtJ TMS I
Transmembrane-Region 2 -> 22  
UniProt: Helical;; Non-Experimental Qualifier: potential;
Mutagenesis-Variant 4  
[Higashi08, UniProt11a]
UniProt: Decrease in spermidine excretion.
Mutagenesis-Variant 5  
[Higashi08, UniProt11a]
UniProt: Decrease in spermidine excretion.
Mutagenesis-Variant 15  
[Higashi08, UniProt11a]
UniProt: Decrease in spermidine excretion.
Transmembrane-Region 32 -> 52 MdtJ TMS II
Mutagenesis-Variant 45  
[Higashi08, UniProt11a]
UniProt: Decrease in spermidine excretion.
Transmembrane-Region 55 -> 75  
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 58 -> 80 MdtJ TMS III
Mutagenesis-Variant 61  
[Higashi08, UniProt11a]
UniProt: Decrease in spermidine excretion.
Mutagenesis-Variant 82  
[Higashi08, UniProt11a]
UniProt: Decrease in spermidine excretion.
Transmembrane-Region 82 -> 102  
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 85 -> 106 MdtJ TMS IV

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bausch04: Bausch C, Ramsey M, Conway T (2004). "Transcriptional organization and regulation of the L-idonic acid pathway (GntII system) in Escherichia coli." J Bacteriol 186(5);1388-97. PMID: 14973046

Brown03: Brown JM, Shaw KJ (2003). "A novel family of Escherichia coli toxin-antitoxin gene pairs." J Bacteriol 185(22);6600-8. PMID: 14594833

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Drew02: Drew D, Sjostrand D, Nilsson J, Urbig T, Chin CN, de Gier JW, von Heijne G (2002). "Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis." Proc Natl Acad Sci U S A 99(5);2690-5. PMID: 11867724

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Hayes06: Hayes ET, Wilks JC, Sanfilippo P, Yohannes E, Tate DP, Jones BD, Radmacher MD, BonDurant SS, Slonczewski JL (2006). "Oxygen limitation modulates pH regulation of catabolism and hydrogenases, multidrug transporters, and envelope composition in Escherichia coli K-12." BMC Microbiol 6;89. PMID: 17026754

Higashi08: Higashi K, Ishigure H, Demizu R, Uemura T, Nishino K, Yamaguchi A, Kashiwagi K, Igarashi K (2008). "Identification of a Spermidine Excretion Protein Complex (MdtJI) in Escherichia coli." J Bacteriol 190(3):872-8. PMID: 18039771

Hirakawa03a: Hirakawa H, Nishino K, Hirata T, Yamaguchi A (2003). "Comprehensive studies of drug resistance mediated by overexpression of response regulators of two-component signal transduction systems in Escherichia coli." J Bacteriol 185(6);1851-6. PMID: 12618449

Hirakawa06: Hirakawa H, Inazumi Y, Senda Y, Kobayashi A, Hirata T, Nishino K, Yamaguchi A (2006). "N-acetyl-d-glucosamine induces the expression of multidrug exporter genes, mdtEF, via catabolite activation in Escherichia coli." J Bacteriol 188(16);5851-8. PMID: 16885453

Jack00a: Jack DL, Storms ML, Tchieu JH, Paulsen IT, Saier MH (2000). "A broad-specificity multidrug efflux pump requiring a pair of homologous SMR-type proteins." J Bacteriol 2000;182(8);2311-3. PMID: 10735877

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kobayashi06a: Kobayashi A, Hirakawa H, Hirata T, Nishino K, Yamaguchi A (2006). "Growth phase-dependent expression of drug exporters in Escherichia coli and its contribution to drug tolerance." J Bacteriol 188(16);5693-703. PMID: 16885437

Nishino01: Nishino K, Yamaguchi A (2001). "Analysis of a complete library of putative drug transporter genes in Escherichia coli." J Bacteriol 2001;183(20);5803-12. PMID: 11566977

Nishino02: Nishino K, Yamaguchi A (2002). "EvgA of the two-component signal transduction system modulates production of the yhiUV multidrug transporter in Escherichia coli." J Bacteriol 184(8);2319-23. PMID: 11914367

Paulsen96a: Paulsen IT, Skurray RA, Tam R, Saier MH, Turner RJ, Weiner JH, Goldberg EB, Grinius LL (1996). "The SMR family: a novel family of multidrug efflux proteins involved with the efflux of lipophilic drugs." Mol Microbiol 1996;19(6);1167-75. PMID: 8730859

Rapp06: Rapp M, Granseth E, Seppala S, von Heijne G (2006). "Identification and evolution of dual-topology membrane proteins." Nat Struct Mol Biol 13(2);112-6. PMID: 16429150

Sabina03: Sabina J, Dover N, Templeton LJ, Smulski DR, Soll D, LaRossa RA (2003). "Interfering with different steps of protein synthesis explored by transcriptional profiling of Escherichia coli K-12." J Bacteriol 185(20);6158-70. PMID: 14526028

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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