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Escherichia coli K-12 substr. MG1655 Polypeptide: Qin prophage; predicted protein



Gene: flxA Accession Numbers: G6833 (EcoCyc), b1566, ECK1560

Regulation Summary Diagram: ?

Summary:
An flxA mutant does not exhibit a defect in flagellar motility [Ide97].

Transcription of flxA is regulated by the flagellar sigma factor, FliA [Ide97].

FlxA: "FliA-dependent expression" [Ide97]

Map Position: [1,644,429 -> 1,644,761] (35.44 centisomes)
Length: 333 bp / 110 aa

Molecular Weight of Polypeptide: 12.208 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0005234 , DIP:DIP-9667N , EchoBASE:EB3203 , EcoGene:EG13429 , EcoliWiki:b1566 , Mint:MINT-1263166 , OU-Microarray:b1566 , PortEco:flxA , PR:PRO_000022674 , Pride:P77609 , Protein Model Portal:P77609 , RefSeq:NP_416084 , RegulonDB:G6833 , String:511145.b1566 , UniProt:P77609

Relationship Links: InterPro:IN-FAMILY:IPR025577 , Pfam:IN-FAMILY:PF14282

Genetic Regulation Schematic: ?

GO Terms:

Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14, Butland05]

MultiFun Terms: extrachromosomal prophage genes and phage related functions

Essentiality data for flxA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 03-Jul-2014 by Keseler I , SRI International


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Ide97: Ide N, Kutsukake K (1997). "Identification of a novel Escherichia coli gene whose expression is dependent on the flagellum-specific sigma factor, FliA, but dispensable for motility development." Gene 1997;199(1-2);19-23. PMID: 9358034

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

Other References Related to Gene Regulation

Bradley07: Bradley MD, Beach MB, de Koning AP, Pratt TS, Osuna R (2007). "Effects of Fis on Escherichia coli gene expression during different growth stages." Microbiology 153(Pt 9);2922-40. PMID: 17768236

Park01: Park K, Choi S, Ko M, Park C (2001). "Novel sigmaF-dependent genes of Escherichia coli found using a specified promoter consensus." FEMS Microbiol Lett 2001;202(2);243-50. PMID: 11520622

Yu06a: Yu HH, Di Russo EG, Rounds MA, Tan M (2006). "Mutational analysis of the promoter recognized by Chlamydia and Escherichia coli sigma(28) RNA polymerase." J Bacteriol 188(15);5524-31. PMID: 16855242

Yu06c: Yu HH, Kibler D, Tan M (2006). "In Silico Prediction and Functional Validation of {sigma}28-Regulated Genes in Chlamydia and Escherichia coli." J Bacteriol 188(23);8206-8212. PMID: 16997971

Zhao07a: Zhao K, Liu M, Burgess RR (2007). "Adaptation in bacterial flagellar and motility systems: from regulon members to 'foraging'-like behavior in E. coli." Nucleic Acids Res 35(13);4441-52. PMID: 17576668


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, BIOCYC13A.