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Escherichia coli K-12 substr. MG1655 Enzyme: phospho-AI-2 isomerase



Gene: lsrG Accession Numbers: G6805 (EcoCyc), b1518, ECK1511

Synonyms: yneC

Regulation Summary Diagram: ?

Regulation summary diagram for lsrG

Summary:
LsrG is an enzyme involved in the degradation of the quorum-sensing regulator Autoinducer 2 (AI-2). Purified LsrG degrades phospho-AI-2 (4-hydroxy-2,3-pentanedione 5-phosphate) [Xavier07]. The reaction products were later shown to be a mixture of 3-hydroxy-2,4-pentanedione 5-phosphate (P-HPD) and 3,4,4-trihydroxy-2-pentanone 5-phosphate (P-TPO); 3,4-dihydroxypent-3-en-2-one 5-phosphate was proposed to be a reaction intermediate [Marques11]. It is now thought that LsrG catalyzes the isomerization reaction from phospho-AI-2 to P-HPD, and that P-HPD exists in equilibrium with its hydrated form, P-TPO [Marques14].

A crystal structure of the enzyme has been solved at 1.8 Å resolution. Mutants in predicted active site residues N25, E54, H65, H70 have reduced or no catalytic activity in vitro [Marques11].

LsrG: "luxS regulated" [Taga01]

Citations: [Quan12a, Pereira12, Brito13]

Gene Citations: [Wang05, Xavier05, Wang05a]

Locations: cytosol

Map Position: [1,605,023 -> 1,605,313] (34.59 centisomes, 125°)
Length: 291 bp / 96 aa

Molecular Weight of Polypeptide: 11.255 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0005067 , DIP:DIP-47853N , EchoBASE:EB3572 , EcoGene:EG13811 , EcoliWiki:b1518 , ModBase:P64461 , OU-Microarray:b1518 , PortEco:lsrG , PR:PRO_000023132 , Protein Model Portal:P64461 , RefSeq:NP_416035 , RegulonDB:G6805 , SMR:P64461 , String:511145.b1518 , UniProt:P64461

Relationship Links: InterPro:IN-FAMILY:IPR007138 , InterPro:IN-FAMILY:IPR011008 , PDB:Structure:3QMQ , Pfam:IN-FAMILY:PF03992 , Prosite:IN-FAMILY:PS51725

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for lsrG

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred from experiment [Marques11, Xavier07]
GO:0009372 - quorum sensing Inferred by curator
Molecular Function: GO:0016853 - isomerase activity Inferred from experiment [Marques11]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: information transfer

Essentiality data for lsrG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 09-Oct-2014 by Keseler I , SRI International


Enzymatic reaction of: 4-hydroxy-2,3-pentanedione-5-phosphate isomerase (phospho-AI-2 isomerase)

EC Number: 5.3.1.-

(4S)-4-hydroxy-2,3-pentanedione 5-phosphate <=> 3-hydroxy-2,4-pentanedione 5-phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: autoinducer AI-2 degradation


Enzymatic reaction of: Autoinducer-2 degrading protein (phospho-AI-2 isomerase)

3-hydroxy-2,4-pentanedione 5-phosphate + H2O <=> 3,4,4-trihydroxy-2-pentanone 5-phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.


Sequence Features

Protein sequence of phospho-AI-2 isomerase with features indicated

Feature Class Location Citations Comment
Conserved-Region 2 -> 91
[UniProt14]
UniProt: ABM.


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Brito13: Brito PH, Rocha EP, Xavier KB, Gordo I (2013). "Natural genome diversity of AI-2 quorum sensing in Escherichia coli: conserved signal production but labile signal reception." Genome Biol Evol 5(1);16-30. PMID: 23246794

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Marques11: Marques JC, Lamosa P, Russell C, Ventura R, Maycock C, Semmelhack MF, Miller ST, Xavier KB (2011). "Processing the interspecies quorum-sensing signal autoinducer-2 (AI-2): characterization of phospho-(S)-4,5-dihydroxy-2,3-pentanedione isomerization by LsrG protein." J Biol Chem 286(20);18331-43. PMID: 21454635

Marques14: Marques JC, Oh IK, Ly DC, Lamosa P, Ventura MR, Miller ST, Xavier KB (2014). "LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2." Proc Natl Acad Sci U S A 111(39);14235-40. PMID: 25225400

Pereira12: Pereira CS, Santos AJ, Bejerano-Sagie M, Correia PB, Marques JC, Xavier KB (2012). "Phosphoenolpyruvate phosphotransferase system regulates detection and processing of the quorum sensing signal autoinducer-2." Mol Microbiol 84(1);93-104. PMID: 22384939

Quan12a: Quan DN, Bentley WE (2012). "Gene network homology in prokaryotes using a similarity search approach: queries of quorum sensing signal transduction." PLoS Comput Biol 8(8);e1002637. PMID: 22916001

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Taga01: Taga ME, Semmelhack JL, Bassler BL (2001). "The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in Salmonella typhimurium." Mol Microbiol 42(3);777-93. PMID: 11722742

UniProt14: UniProt Consortium (2014). "UniProt version 2014-08 released on 2014-08-14 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wang05: Wang L, Hashimoto Y, Tsao CY, Valdes JJ, Bentley WE (2005). "Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli." J Bacteriol 187(6);2066-76. PMID: 15743955

Wang05a: Wang L, Li J, March JC, Valdes JJ, Bentley WE (2005). "luxS-dependent gene regulation in Escherichia coli K-12 revealed by genomic expression profiling." J Bacteriol 187(24);8350-60. PMID: 16321939

Xavier05: Xavier KB, Bassler BL (2005). "Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli." J Bacteriol 187(1);238-48. PMID: 15601708

Xavier07: Xavier KB, Miller ST, Lu W, Kim JH, Rabinowitz J, Pelczer I, Semmelhack MF, Bassler BL (2007). "Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria." ACS Chem Biol 2(2);128-36. PMID: 17274596

Other References Related to Gene Regulation

GamaCastro08: Gama-Castro S, Jimenez-Jacinto V, Peralta-Gil M, Santos-Zavaleta A, Penaloza-Spinola MI, Contreras-Moreira B, Segura-Salazar J, Muniz-Rascado L, Martinez-Flores I, Salgado H, Bonavides-Martinez C, Abreu-Goodger C, Rodriguez-Penagos C, Miranda-Rios J, Morett E, Merino E, Huerta AM, Trevino-Quintanilla L, Collado-Vides J (2008). "RegulonDB (version 6.0): gene regulation model of Escherichia coli K-12 beyond transcription, active (experimental) annotated promoters and Textpresso navigation." Nucleic Acids Res 36(Database issue);D120-4. PMID: 18158297

Xue09: Xue T, Zhao L, Sun H, Zhou X, Sun B (2009). "LsrR-binding site recognition and regulatory characteristics in Escherichia coli AI-2 quorum sensing." Cell Res 19(11);1258-68. PMID: 19636340


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sun Apr 26, 2015, biocyc13.