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Escherichia coli K-12 substr. MG1655 Polypeptide: biofilm-dependent modulation protein



Gene: bdm Accession Numbers: G6776 (EcoCyc), b1481, ECK1475

Synonyms: yddX, f92

Regulation Summary Diagram: ?

Regulation summary diagram for bdm

Summary:
Bdm appears to be involved in regulating flagellar biosynthesis [Kim14].

bdm is a member of the Rcs regulon [Hagiwara03, FrancezCharlot05]. It was originally reported that expression of bdm is reduced within biofilms and decreased by high osmolarity [PrigentCombaret99]. However, other studies agree that expression is increased by high osmolarity [Weber02, FrancezCharlot05, Shabala09] and that the increase is RcsB-dependent [FrancezCharlot05]. Expression of bdm is also upregulated by acid treatment [Kannan08]. In addition, overexpression of bdm from a multicopy plasmid leads to enhanced biofilm formation [Sim10].

bdm mRNA is degraded by RNase III; the sustained increase in bdm mRNA levels upon osmotic upshift requires downregulation of RNase III activity [Sim10].

Gene expression analysis of a bdm mutant compared to wild type shows downregulation of a number of genes involved in flagellar biosynthesis, chemotaxis and motility. A bdm deletion strain does not have a growth defect, but shows impaired motility [Kim14].

Bdm: "biofilm-dependent modulation" [FrancezCharlot05]

Citations: [Kim11c]

Map Position: [1,554,089 <- 1,554,304] (33.5 centisomes, 121°)
Length: 216 bp / 71 aa

Molecular Weight of Polypeptide: 7.987 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004938 , EchoBASE:EB4048 , EcoGene:EG14301 , Ecol199310Cyc:C1914 , EcoliWiki:b1481 , OU-Microarray:b1481 , PortEco:bdm , PR:PRO_000022206 , Protein Model Portal:P76127 , RefSeq:NP_415998 , RegulonDB:G6776 , String:511145.b1481 , UniProt:P76127

Relationship Links: InterPro:IN-FAMILY:IPR019625 , Pfam:IN-FAMILY:PF10684

Genetic Regulation Schematic: ?

Genetic regulation schematic for bdm

GO Terms:

Biological Process: GO:1902210 - positive regulation of bacterial-type flagellum assembly Inferred from experiment [Kim14]

MultiFun Terms: cell processes motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)

Essentiality data for bdm knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 19-Nov-2014 by Keseler I , SRI International


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

FrancezCharlot05: Francez-Charlot A, Castanie-Cornet MP, Gutierrez C, Cam K (2005). "Osmotic Regulation of the Escherichia coli bdm (Biofilm-Dependent Modulation) Gene by the RcsCDB His-Asp Phosphorelay." J Bacteriol 187(11);3873-7. PMID: 15901715

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Hagiwara03: Hagiwara D, Sugiura M, Oshima T, Mori H, Aiba H, Yamashino T, Mizuno T (2003). "Genome-wide analyses revealing a signaling network of the RcsC-YojN-RcsB phosphorelay system in Escherichia coli." J Bacteriol 185(19);5735-46. PMID: 13129944

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kannan08: Kannan G, Wilks JC, Fitzgerald DM, Jones BD, Bondurant SS, Slonczewski JL (2008). "Rapid acid treatment of Escherichia coli: transcriptomic response and recovery." BMC Microbiol 8;37. PMID: 18302792

Kim11c: Kim K, Sim SH, Jeon CO, Lee Y, Lee K (2011). "Base substitutions at scissile bond sites are sufficient to alter RNA-binding and cleavage activity of RNase III." FEMS Microbiol Lett 315(1);30-7. PMID: 21133991

Kim14: Kim JS, Kim YJ, Seo S, Seong MJ, Lee K (2014). "Functional Role of bdm During Flagella Biogenesis in Escherichia coli." Curr Microbiol. PMID: 25398323

PrigentCombaret99: Prigent-Combaret C, Vidal O, Dorel C, Lejeune P (1999). "Abiotic surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli." J Bacteriol 181(19);5993-6002. PMID: 10498711

Shabala09: Shabala L, Bowman J, Brown J, Ross T, McMeekin T, Shabala S (2009). "Ion transport and osmotic adjustment in Escherichia coli in response to ionic and non-ionic osmotica." Environ Microbiol 11(1);137-48. PMID: 18793315

Sim10: Sim SH, Yeom JH, Shin C, Song WS, Shin E, Kim HM, Cha CJ, Han SH, Ha NC, Kim SW, Hahn Y, Bae J, Lee K (2010). "Escherichia coli ribonuclease III activity is downregulated by osmotic stress: consequences for the degradation of bdm mRNA in biofilm formation." Mol Microbiol 75(2);413-25. PMID: 19943899

Weber02: Weber A, Jung K (2002). "Profiling early osmostress-dependent gene expression in Escherichia coli using DNA macroarrays." J Bacteriol 184(19);5502-7. PMID: 12218039

Other References Related to Gene Regulation

Kamenšek13: Kamenšek S, Žgur-Bertok D (2013). "Global transcriptional responses to the bacteriocin colicin M in Escherichia coli." BMC Microbiol 13;42. PMID: 23421615


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue May 26, 2015, biocyc13.