Escherichia coli K-12 substr. MG1655 Polypeptide: nucleotide binding filament protein

Gene: uspF Accession Numbers: G6699 (EcoCyc), b1376, ECK1371

Synonyms: yzzL, ynaF, UP03

Regulation Summary Diagram: ?

Regulation summary diagram for uspF

UspF is a nucleotide binding protein in the class II universal stress protein family [Saveanu02]. Based on sequence similarity, UspF is expected to dimerize and to have an ATP-binding site like that of Methanococcus jannaschii MJ0577 protein [Saveanu02].

UspF promotes adhesion at the expense of motility. A single deletion of uspF or uspG has a dominant negative effect on fimbria-dependent adhesion, while the uspFG double mutant shows no adhesion phenotype. A uspF mutant is more sensitive to the antibiotic streptonigrin; the effect of a uspFG double mutant is additive [Nachin05].

UspF is upregulated under glucose-limited fed-batch conditions [Raman05].

Map Position: [1,433,209 <- 1,433,643] (30.89 centisomes, 111°)
Length: 435 bp / 144 aa

Molecular Weight of Polypeptide: 16.017 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004605 , DIP:DIP-12739N , EchoBASE:EB2539 , EcoGene:EG12674 , EcoliWiki:b1376 , ModBase:P37903 , OU-Microarray:b1376 , PortEco:uspF , PR:PRO_000024195 , Pride:P37903 , Protein Model Portal:P37903 , RefSeq:NP_415894 , RegulonDB:G6699 , SMR:P37903 , String:511145.b1376 , UniProt:P37903

Relationship Links: InterPro:IN-FAMILY:IPR006015 , InterPro:IN-FAMILY:IPR006016 , InterPro:IN-FAMILY:IPR014729 , Pfam:IN-FAMILY:PF00582 , Prints:IN-FAMILY:PR01438

In Paralogous Gene Group: 277 (3 members)

GO Terms:

Biological Process: GO:0006979 - response to oxidative stress Inferred from experiment [Nachin05]
GO:0007155 - cell adhesion Inferred from experiment [Nachin05]
GO:0006950 - response to stress Inferred by computational analysis [GOA01]
Molecular Function: GO:0005524 - ATP binding Inferred from experiment [Saveanu02]


Essentiality data for uspF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 10-Nov-2006 by Keseler I , SRI International

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Nachin05: Nachin L, Nannmark U, Nystrom T (2005). "Differential roles of the universal stress proteins of Escherichia coli in oxidative stress resistance, adhesion, and motility." J Bacteriol 187(18);6265-72. PMID: 16159758

Raman05: Raman B, Nandakumar MP, Muthuvijayan V, Marten MR (2005). "Proteome analysis to assess physiological changes in Escherichia coli grown under glucose-limited fed-batch conditions." Biotechnol Bioeng 92(3);384-92. PMID: 16180237

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Saveanu02: Saveanu C, Miron S, Borza T, Craescu CT, Labesse G, Gagyi C, Popescu A, Schaeffer F, Namane A, Laurent-Winter C, Barzu O, Gilles AM (2002). "Structural and nucleotide-binding properties of YajQ and YnaF, two Escherichia coli proteins of unknown function." Protein Sci 11(11);2551-60. PMID: 12381839

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 13, 2015, BIOCYC14B.