|Gene:||lolC||Accession Numbers: G6573 (EcoCyc), b1116, ECK1102|
Component of: LolCDE ABC lipoprotein transporter (extended summary available)
LolC is the predicted membrane component of the LolCDE lipoprotein transporter [Yakushi00]. LolCDE is a unique member of the ATP-binding cassette (ABC) superfamily of transporters.
Locations: inner membrane
|Map Position: [1,174,650 -> 1,175,849] (25.32 centisomes, 91°)||Length: 1200 bp / 399 aa|
Molecular Weight of Polypeptide: 43.264 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0003772 , DIP:DIP-58651N , EchoBASE:EB3213 , EcoGene:EG13439 , EcoliWiki:b1116 , OU-Microarray:b1116 , PortEco:lolC , PR:PRO_000023105 , Pride:P0ADC3 , Protein Model Portal:P0ADC3 , RefSeq:NP_415634 , RegulonDB:G6573 , String:511145.b1116 , UniProt:P0ADC3
In Paralogous Gene Group: 189 (15 members)
|Biological Process:||GO:0042953 - lipoprotein transport
GO:0006810 - transport [UniProtGOA11a]
|Molecular Function:||GO:0042954 - lipoprotein transporter activity [GOA01a, Yakushi00]|
|Cellular Component:||GO:0005886 - plasma membrane
[UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, Daley05]
GO:0005887 - integral component of plasma membrane [Yakushi00]
GO:0043234 - protein complex [Yakushi00]
GO:0016020 - membrane [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane [UniProtGOA11a, GOA01a]
|MultiFun Terms:||cell structure → membrane|
|transport → Channel-type Transporters → Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters → The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases → ABC superfamily, membrane component|
|Growth Medium||Growth?||T (°C)||O2||pH||Osm/L||Growth Observations|
|LB Lennox||No||37||Aerobic||7||No [Baba06, Comment 1]|
Subunit of: LolCDE ABC lipoprotein transporter
Subunit composition of LolCDE ABC lipoprotein transporter = [LolE][LolD]2[LolC]
Localization of lipoproteins to the periplasmic surface of the outer membrane in Escherichia coli requires a lipoprotein-specific sorting complex, made up of five proteins (LolABCDE) [Narita04].
Lipoproteins in E.coli, as well as in other bacteria, are membrane proteins that have covalently-attached lipids at their amino-terminal cysteine residue. Within the bacterial envelope of E. coli there are more than 90 species of lipoproteins, located on the periplasmic surfaces of both the inner and outer membrane, and responsible for a variety of functions. [Narita04].
All lipoproteins are thought to be synthesized with an N-terminal signal sequence which allows for their targeting to the Sec secretion system and subsequent translocation across the cytoplasmic membrane [Duong97b]. After translocation across the cytoplasmic membrane, the signal peptide sequence is cleaved by lipoprotein-specific signal peptidase II followed by aminoacylation of the terminal cysteine residue [Pugsley93]. The mature form of the lipoprotein is then localized to either the inner or outer membrane. If the amino-acid residue next to the terminal cysteine is aspartate, then the lipoprotein is localized to the inner membrane. In all other cases, the lipoprotein is targeted for translocation across the periplasmic space to the outer membrane [Yamaguchi88].
LolA is a periplasmic proteinous factor which serves as a hydrophobic container, allowing for lipoproteins to become solubilized, thereby facilitating their translocation across the hydrophilic periplasmic space [Takeda03]. LolB is an outer membrane receptor for the LolA-lipoprotein complex [Yokota99]. LolCDE is a unique ABC transporter involved in the export of lipoproteins to the outer membrane [Yakushi00]. Sequence analysis indicates that LolC and LolE are integral membrane proteins and LolD is an ABC ATPase. In in vivo photo crosslinking studies LolA was observed to generate cross linked products with LolC but not with LolE suggesting that the two integral membrane components may have distinct functions [Okuda09]. LolCDE has been shown to be capable of utilizing ATP, GTP or UTP in the release process [Yakushi98], [Yakushi00].
Locations: inner membrane
|Biological Process:||GO:0042953 - lipoprotein transport [Yakushi00]|
|Molecular Function:||GO:0042954 - lipoprotein transporter activity [Yakushi00]|
|Cellular Component:||GO:0005887 - integral component of plasma membrane
GO:0043234 - protein complex [Yakushi00]
|Transmembrane-Region||25 -> 45|
|Transmembrane-Region||270 -> 290|
|Transmembrane-Region||310 -> 330|
|Transmembrane-Region||358 -> 378|
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Okuda09: Okuda S, Tokuda H (2009). "Model of mouth-to-mouth transfer of bacterial lipoproteins through inner membrane LolC, periplasmic LolA, and outer membrane LolB." Proc Natl Acad Sci U S A 106(14);5877-82. PMID: 19307584
Takeda03: Takeda K, Miyatake H, Yokota N, Matsuyama S, Tokuda H, Miki K (2003). "Crystal structures of bacterial lipoprotein localization factors, LolA and LolB." EMBO J 22(13);3199-209. PMID: 12839983
Yakushi00: Yakushi T, Masuda K, Narita S, Matsuyama S, Tokuda H (2000). "A new ABC transporter mediating the detachment of lipid-modified proteins from membranes." Nat Cell Biol 2(4);212-8. PMID: 10783239
Yakushi98: Yakushi T, Yokota N, Matsuyama S, Tokuda H (1998). "LolA-dependent release of a lipid-modified protein from the inner membrane of Escherichia coli requires nucleoside triphosphate." J Biol Chem 273(49);32576-81. PMID: 9829994
Yasuda09: Yasuda M, Iguchi-Yokoyama A, Matsuyama S, Tokuda H, Narita S (2009). "Membrane topology and functional importance of the periplasmic region of ABC transporter LolCDE." Biosci Biotechnol Biochem 73(10);2310-6. PMID: 19809197
Yokota99: Yokota N, Kuroda T, Matsuyama S, Tokuda H (1999). "Characterization of the LolA-LolB system as the general lipoprotein localization mechanism of Escherichia coli." J Biol Chem 274(43);30995-9. PMID: 10521496
Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111
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