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Escherichia coli K-12 substr. MG1655 Polypeptide: regulator of biofilm formation



Gene: bssS Accession Numbers: G6557 (EcoCyc), b1060, ECK1045

Synonyms: yceP

Regulation Summary Diagram: ?

Summary:
There have been contradictory reports on the effect of a bssS mutation on biofilm formation. [Beloin04] reported that a bssS mutant is impaired in biofilm formation, while [Domka06] reported that deletion of bssS leads to increased biofilm formation in LB+glucose and MC9C+glucose media and increased motility in LB medium.

A bssS deletion mutant does not have a significant growth defect. Deletion of bssS affects the expression of more than 400 genes significantly. Among the affected pathways are indole transport and AI-2 uptake and processing. A regulatory model has been proposed [Domka06].

Transcription of bssS is induced upon biofilm formation compared to planktonic growth in both exponential and stationary phase [Schembri03, Beloin04, Ren04a]. Induction of expression was found to be dependent on the presence of the F plasmid [Beloin04].

BssS: "regulator of biofilm through signal secretion" [Domka06]

Map Position: [1,119,924 <- 1,120,178] (24.14 centisomes)
Length: 255 bp / 84 aa

Molecular Weight of Polypeptide: 9.664 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003594 , EchoBASE:EB4081 , EcoGene:EG14335 , EcoliWiki:b1060 , OU-Microarray:b1060 , PortEco:bssS , PR:PRO_000022233 , Protein Model Portal:P0AB33 , RefSeq:NP_415578 , RegulonDB:G6557 , String:511145.b1060 , UniProt:P0AB33

Relationship Links: InterPro:IN-FAMILY:IPR025730 , Pfam:IN-FAMILY:PF13991 , ProDom:IN-FAMILY:PD060858

GO Terms:

Biological Process: GO:1900190 - regulation of single-species biofilm formation Inferred from experiment [Beloin04, Domka06]

MultiFun Terms: information transfer

Essentiality data for bssS knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 11-Apr-2006 by Keseler I , SRI International


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Beloin04: Beloin C, Valle J, Latour-Lambert P, Faure P, Kzreminski M, Balestrino D, Haagensen JA, Molin S, Prensier G, Arbeille B, Ghigo JM (2004). "Global impact of mature biofilm lifestyle on Escherichia coli K-12 gene expression." Mol Microbiol 51(3);659-74. PMID: 14731270

Domka06: Domka J, Lee J, Wood TK (2006). "YliH (BssR) and YceP (BssS) Regulate Escherichia coli K-12 Biofilm Formation by Influencing Cell Signaling." Appl Environ Microbiol 72(4);2449-59. PMID: 16597943

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Ren04a: Ren D, Bedzyk LA, Thomas SM, Ye RW, Wood TK (2004). "Gene expression in Escherichia coli biofilms." Appl Microbiol Biotechnol 64(4);515-24. PMID: 14727089

Schembri03: Schembri MA, Kjaergaard K, Klemm P (2003). "Global gene expression in Escherichia coli biofilms." Mol Microbiol 48(1);253-67. PMID: 12657059

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Other References Related to Gene Regulation

Maciag11a: Maciag A, Peano C, Pietrelli A, Egli T, De Bellis G, Landini P (2011). "In vitro transcription profiling of the {sigma}S subunit of bacterial RNA polymerase: re-definition of the {sigma}S regulon and identification of {sigma}S-specific promoter sequence elements." Nucleic Acids Res 39(13);5338-55. PMID: 21398637

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305

Nonaka06: Nonaka G, Blankschien M, Herman C, Gross CA, Rhodius VA (2006). "Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat stress." Genes Dev 20(13);1776-89. PMID: 16818608

Wade06: Wade JT, Roa DC, Grainger DC, Hurd D, Busby SJ, Struhl K, Nudler E (2006). "Extensive functional overlap between sigma factors in Escherichia coli." Nat Struct Mol Biol 13(9);806-14. PMID: 16892065

Zhao05: Zhao K, Liu M, Burgess RR (2005). "The global transcriptional response of Escherichia coli to induced sigma 32 protein involves sigma 32 regulon activation followed by inactivation and degradation of sigma 32 in vivo." J Biol Chem 280(18);17758-68. PMID: 15757896


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, biocyc13.