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Escherichia coli K-12 substr. MG1655 Enzyme: triphosphoribosyl-dephospho-CoA synthase



Gene: citG Accession Numbers: G6339 (EcoCyc), b0613, ECK0606

Synonyms: ybdT

Regulation Summary Diagram: ?

Summary:
CitG catalyzes the synthesis of the citrate lyase prosthetic group precursor, 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA, from dephospho-CoA and ATP. The enzyme does not catalyze the conversion of AMP-ACP to holo-ACP [Schneider00b, Schneider00a]

Locations: cytosol

Map Position: [645,854 <- 646,732] (13.92 centisomes)
Length: 879 bp / 292 aa

Molecular Weight of Polypeptide: 31.644 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002114 , EchoBASE:EB3309 , EcoGene:EG13539 , EcoliWiki:b0613 , OU-Microarray:b0613 , PortEco:citG , PR:PRO_000022291 , Pride:P77231 , Protein Model Portal:P77231 , RefSeq:NP_415146 , RegulonDB:G6339 , String:511145.b0613 , UniProt:P77231

Relationship Links: InterPro:IN-FAMILY:IPR002736 , InterPro:IN-FAMILY:IPR017551 , Pfam:IN-FAMILY:PF01874

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0051191 - prosthetic group biosynthetic process Inferred from experiment [Schneider00b]
GO:0016310 - phosphorylation Inferred by computational analysis [GOA01a]
Molecular Function: GO:0046917 - triphosphoribosyl-dephospho-CoA synthase activity Inferred from experiment Inferred by computational analysis [GOA01a, Schneider00a]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer protein related

Essentiality data for citG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Last-Curated ? 12-Jun-2013 by Keseler I , SRI International


Enzymatic reaction of: triphosphoribosyl-dephospho-CoA synthase

EC Number: 2.4.2.52

3'-dephospho-CoA + ATP + H+ <=> 2'-(5''-triphospho-α-D-ribosyl)-3'-dephospho-CoA + adenine

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: citrate lyase activation

T(opt): 20 °C [BRENDA14, Schneider00a]


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Schneider00a: Schneider K, Dimroth P, Bott M (2000). "Identification of triphosphoribosyl-dephospho-CoA as precursor of the citrate lyase prosthetic group." FEBS Lett 2000;483(2-3);165-8. PMID: 11042274

Schneider00b: Schneider K, Dimroth P, Bott M (2000). "Biosynthesis of the prosthetic group of citrate lyase." Biochemistry 2000;39(31);9438-50. PMID: 10924139

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Bobon: Bobon J, Parent M, Toussaint C, Pinchard A "[Long-acting neuroleptics. IV. Preliminary study of clopimozide (R 29764)]." Acta Psychiatr Belg 76(1);138-43. PMID: 970182

Ingmer98: Ingmer H, Miller CA, Cohen SN (1998). "Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two-component system regulator." Mol Microbiol 29(1);49-59. PMID: 9701802

Scheu12: Scheu PD, Witan J, Rauschmeier M, Graf S, Liao YF, Ebert-Jung A, Basche T, Erker W, Unden G (2012). "CitA/CitB two-component system regulating citrate fermentation in Escherichia coli and its relation to the DcuS/DcuR system in vivo." J Bacteriol 194(3);636-45. PMID: 22101843

Yamamoto08a: Yamamoto K, Matsumoto F, Oshima T, Fujita N, Ogasawara N, Ishihama A (2008). "Anaerobic regulation of citrate fermentation by CitAB in Escherichia coli." Biosci Biotechnol Biochem 72(11);3011-4. PMID: 18997424


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC13B.