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Escherichia coli K-12 substr. MG1655 Polypeptide: endodeoxyribonuclease RUS (Holliday junction resolvase)



Gene: rusA Accession Numbers: G6306 (EcoCyc), b0550, ECK0541

Synonyms: ybcP, rus

Regulation Summary Diagram: ?

Summary:
The RusA protein is an endonuclease that functions as a Holliday junction resolvase [Sharples94].

Locations: cytosol

Map Position: [572,594 -> 572,956] (12.34 centisomes)
Length: 363 bp / 120 aa

Molecular Weight of Polypeptide: 13.846 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001880 , EchoBASE:EB4170 , EcoGene:EG20283 , EcoliWiki:b0550 , ModBase:P0AG74 , OU-Microarray:b0550 , PortEco:rusA , PR:PRO_000023897 , Protein Model Portal:P0AG74 , RefSeq:NP_415082 , RegulonDB:G6306 , SMR:P0AG74 , String:511145.b0550 , Swiss-Model:P0AG74 , UniProt:P0AG74

Relationship Links: InterPro:IN-FAMILY:IPR008822 , InterPro:IN-FAMILY:IPR016281 , PDB:Structure:1Q8R , PDB:Structure:2H8C , PDB:Structure:2H8E , Pfam:IN-FAMILY:PF05866

GO Terms:

Biological Process: GO:0000737 - DNA catabolic process, endonucleolytic Inferred by computational analysis Inferred from experiment [Sharples94, GOA01a, GOA01]
GO:0006281 - DNA repair Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01, Sharples94]
GO:0006310 - DNA recombination Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0006974 - cellular response to DNA damage stimulus Inferred by computational analysis [UniProtGOA11a]
GO:0090305 - nucleic acid phosphodiester bond hydrolysis Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0008821 - crossover junction endodeoxyribonuclease activity Inferred from experiment Inferred by computational analysis [GOA01a, GOA01, Sharples94]
GO:0010844 - recombination hotspot binding Inferred from experiment [Sharples94]
GO:0000287 - magnesium ion binding Inferred by computational analysis [GOA01]
GO:0004518 - nuclease activity Inferred by computational analysis [UniProtGOA11a]
GO:0004519 - endonuclease activity Inferred by computational analysis [UniProtGOA11a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0048476 - Holliday junction resolvase complex Inferred from experiment [Sharples94]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: extrachromosomal prophage genes and phage related functions
information transfer DNA related DNA degradation
information transfer DNA related DNA repair
metabolism degradation of macromolecules DNA

Essentiality data for rusA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Sequence Features

Feature Class Location Citations Comment
Protein-Segment 13 -> 16
[UniProt10]
UniProt: DNA binding; Sequence Annotation Type: region of interest;
Mutagenesis-Variant 16
[Bolt00, UniProt11]
Alternate sequence: R → Q; UniProt: No effect on ability to promote DNA repair.
Mutagenesis-Variant 17
[Bolt00, UniProt11]
Alternate sequence: Y → L; UniProt: No effect on ability to promote DNA repair.
Mutagenesis-Variant 19
[Bolt00, UniProt11]
Alternate sequence: R → Q; UniProt: No effect on ability to promote DNA repair.
Mutagenesis-Variant 58
[Bolt00, UniProt11]
Alternate sequence: R → Q; UniProt: No effect on ability to promote DNA repair.
Protein-Segment 66 -> 73
[UniProt10]
UniProt: DNA binding; Sequence Annotation Type: region of interest;
Mutagenesis-Variant 68
[Bolt00, UniProt11]
Alternate sequence: R → Q; UniProt: No effect on ability to promote DNA repair.
Mutagenesis-Variant 69
[Bolt00, UniProt11]
Alternate sequence: R → A; UniProt: Loss of ability to promote DNA repair. Great loss of Holliday junction resolvase activity. No effect on DNA binding.
Alternate sequence: R → Q; UniProt: Loss of ability to promote DNA repair. Great loss of Holliday junction resolvase activity. No effect on DNA binding.
Mutagenesis-Variant 70
[Bolt99, GiraudPanis98, UniProt11]
Alternate sequence: D → N; UniProt: Reduces junction resolution 80- fold. No effect on DNA binding.
Metal-Binding-Site 70
[UniProt10]
UniProt: Magnesium; Non-Experimental Qualifier: probable;
Mutagenesis-Variant 72
[Bolt99, UniProt11]
Alternate sequence: D → N; UniProt: Loss of ability to resolve junctions. No effect on DNA binding.
Metal-Binding-Site 72
[UniProt10]
UniProt: Magnesium; Non-Experimental Qualifier: probable;
Mutagenesis-Variant 73
[Bolt00, UniProt11]
Alternate sequence: N → A; UniProt: Slight reduction in ability to promote DNA repair. Great reduction in Holliday junction resolution activity.
Mutagenesis-Variant 76
[Bolt00, UniProt11]
Alternate sequence: K → R; UniProt: Loss of ability to promote DNA repair. Less than 2% activity of Holliday junction resolvase. No effect on DNA binding.
Alternate sequence: K → Q; UniProt: Loss of ability to promote DNA repair. Loss of Holliday junction resolvase activity. No effect on DNA binding.
Mutagenesis-Variant 80
[Bolt99, UniProt11]
Alternate sequence: D → N; UniProt: Slight reduction in ability to resolve junctions. No effect on DNA binding.
Mutagenesis-Variant 87
[Bolt00, UniProt11]
Alternate sequence: F → V; UniProt: No effect on ability to promote DNA repair.
Alternate sequence: F → Y; UniProt: No effect on ability to promote DNA repair.
Mutagenesis-Variant 90
[Bolt99, UniProt11]
Alternate sequence: D → N; UniProt: Slight reduction in ability to resolve junctions. No effect on DNA binding.
Mutagenesis-Variant 91
[Bolt99, UniProt11]
Alternate sequence: D → N; UniProt: Loss of ability to resolve junctions. No effect on DNA binding.
Metal-Binding-Site 91
[UniProt10]
UniProt: Magnesium; Non-Experimental Qualifier: probable;
Mutagenesis-Variant 101
[Bolt00, UniProt11]
Alternate sequence: K → Q; UniProt: No effect on ability to promote DNA repair.
Mutagenesis-Variant 111
[Bolt99, UniProt11]
Alternate sequence: E → Q; UniProt: No effect on resolvase activity or DNA binding.
Mutagenesis-Variant 116
[Bolt99, UniProt11]
Alternate sequence: E → Q; UniProt: No effect on resolvase activity or DNA binding.


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
3/2/1998 (pkarp) Merged genes G778/rus and G6306/rus
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bolt00: Bolt EL, Sharples GJ, Lloyd RG (2000). "Analysis of conserved basic residues associated with DNA binding (Arg69) and catalysis (Lys76) by the RusA holliday junction resolvase." J Mol Biol 304(2);165-76. PMID: 11080453

Bolt99: Bolt EL, Sharples GJ, Lloyd RG (1999). "Identification of three aspartic acid residues essential for catalysis by the RusA holliday junction resolvase." J Mol Biol 286(2);403-15. PMID: 9973560

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GiraudPanis98: Giraud-Panis MJ, Lilley DM (1998). "Structural recognition and distortion by the DNA junction-resolving enzyme RusA." J Mol Biol 278(1);117-33. PMID: 9571038

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Sharples94: Sharples GJ, Chan SN, Mahdi AA, Whitby MC, Lloyd RG (1994). "Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions." EMBO J 13(24);6133-42. PMID: 7813450

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC13A.