Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Polypeptide: predicted acyl-CoA synthetase with NAD(P)-binding domain and succinyl-CoA synthetase domain



Gene: yahF Accession Numbers: G6185 (EcoCyc), b0320, ECK0318

Regulation Summary Diagram: ?

Summary:
A down mutation in the yahF ribosome binding site increases fitness for growth on 16 g/l acetate [Sandoval12].

Locations: cytosol

Map Position: [336,002 -> 337,549] (7.24 centisomes)
Length: 1548 bp / 515 aa

Molecular Weight of Polypeptide: 55.581 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001099 , DIP:DIP-11258N , EchoBASE:EB3360 , EcoGene:EG13590 , EcoliWiki:b0320 , Mint:MINT-1241539 , ModBase:P77187 , OU-Microarray:b0320 , PortEco:yahF , Pride:P77187 , Protein Model Portal:P77187 , RefSeq:NP_414854 , RegulonDB:G6185 , SMR:P77187 , String:511145.b0320 , UniProt:P77187

Relationship Links: InterPro:IN-FAMILY:IPR003781 , InterPro:IN-FAMILY:IPR005811 , InterPro:IN-FAMILY:IPR016102 , Pfam:IN-FAMILY:PF00549 , Pfam:IN-FAMILY:PF02629

In Paralogous Gene Group: 100 (4 members)

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
Molecular Function: GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0048037 - cofactor binding Inferred by computational analysis [GOA01]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Zhang07]

Gene Class: ORFs

Essentiality data for yahF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Last-Curated ? 03-Jul-2012 by Keseler I , SRI International


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Sandoval12: Sandoval NR, Kim JY, Glebes TY, Reeder PJ, Aucoin HR, Warner JR, Gill RT (2012). "Strategy for directing combinatorial genome engineering in Escherichia coli." Proc Natl Acad Sci U S A 109(26);10540-5. PMID: 22689973

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, biocyc14.