Escherichia coli K-12 substr. MG1655 Polypeptide: predicted acyl-CoA synthetase with NAD(P)-binding domain and succinyl-CoA synthetase domain

Gene: yahF Accession Numbers: G6185 (EcoCyc), b0320, ECK0318

Regulation Summary Diagram: ?

Regulation summary diagram for yahF

A down mutation in the yahF ribosome binding site increases fitness for growth on 16 g/l acetate [Sandoval12].

Locations: cytosol

Map Position: [336,002 -> 337,549] (7.24 centisomes, 26°)
Length: 1548 bp / 515 aa

Molecular Weight of Polypeptide: 55.581 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001099 , DIP:DIP-11258N , EchoBASE:EB3360 , EcoGene:EG13590 , EcoliWiki:b0320 , Mint:MINT-1241539 , ModBase:P77187 , OU-Microarray:b0320 , PortEco:yahF , Pride:P77187 , Protein Model Portal:P77187 , RefSeq:NP_414854 , RegulonDB:G6185 , SMR:P77187 , String:511145.b0320 , UniProt:P77187

Relationship Links: InterPro:IN-FAMILY:IPR003781 , InterPro:IN-FAMILY:IPR005811 , InterPro:IN-FAMILY:IPR016102 , Pfam:IN-FAMILY:PF00549 , Pfam:IN-FAMILY:PF02629

In Paralogous Gene Group: 100 (4 members)

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
Molecular Function: GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0048037 - cofactor binding Inferred by computational analysis [GOA01]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Zhang07]

Gene Class: ORFs

Essentiality data for yahF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Last-Curated ? 03-Jul-2012 by Keseler I , SRI International

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Sandoval12: Sandoval NR, Kim JY, Glebes TY, Reeder PJ, Aucoin HR, Warner JR, Gill RT (2012). "Strategy for directing combinatorial genome engineering in Escherichia coli." Proc Natl Acad Sci U S A 109(26);10540-5. PMID: 22689973

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, BIOCYC14B.