Escherichia coli K-12 substr. MG1655 Polypeptide: IS1 protein InsB

Gene: insB-1 Accession Numbers: G6085 (EcoCyc), b0021, ECK0022

Regulation Summary Diagram: ?

Regulation summary diagram for insB-1

IS1 is the smallest insertion sequence in E. coli. It codes for three proteins, InsA, InsB and InsAB'.

InsAB' is the transposase for IS1 [Escoubas94]. Its translation depends on a -1 frameshift within the 3' end region of insA which allows translational readthrough into insB [Sekine89]. In the absence of this frameshift, the IS1 transcriptional repressor protein InsA is produced instead [Sekine89, Escoubas94, Matsutani97].

Locations: cytosol

Map Position: [19,811 <- 20,314] (0.43 centisomes, 2°)
Length: 504 bp / 167 aa

Molecular Weight of Polypeptide: 19.565 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000075 , EcoGene:EG60002 , EcoliWiki:b0021 , OU-Microarray:b0021 , PortEco:insB-1 , Protein Model Portal:P0CF25 , RefSeq:NP_414562 , RegulonDB:G6085 , UniProt:P0CF25

Relationship Links: EcoO157Cyc:Homolog:Z1123-MONOMER , EcoO157Cyc:Homolog:Z1192-MONOMER , EcoO157Cyc:Homolog:Z1562-MONOMER , EcoO157Cyc:Homolog:Z1632-MONOMER , EcoO157Cyc:Homolog:Z5491-MONOMER , InterPro:IN-FAMILY:IPR005063 , Pfam:IN-FAMILY:PF03400

GO Terms:

Biological Process: GO:0006310 - DNA recombination Inferred by computational analysis [UniProtGOA11a]
GO:0006313 - transposition, DNA-mediated Inferred by computational analysis [GOA01a]
GO:0032196 - transposition Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0003677 - DNA binding Inferred by computational analysis [GOA01a]
GO:0004803 - transposase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: extrachromosomal transposon related

Essentiality data for insB-1 knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]

Last-Curated ? 29-Aug-2006 by Shearer A , SRI International

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Escoubas94: Escoubas JM, Lane D, Chandler M (1994). "Is the IS1 transposase, InsAB', the only IS1-encoded protein required for efficient transposition?." J Bacteriol 176(18);5864-7. PMID: 8083181

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Matsutani97: Matsutani S (1997). "Genetic analyses of the interactions of the IS1-encoded proteins with the left end of IS1 and its insertion hotspot." J Mol Biol 267(3);548-60. PMID: 9126837

Sekine89: Sekine Y, Ohtsubo E (1989). "Frameshifting is required for production of the transposase encoded by insertion sequence 1." Proc Natl Acad Sci U S A 86(12);4609-13. PMID: 2543983

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc14.