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Escherichia coli K-12 substr. MG1655 Polypeptide: small toxic polypeptide LdrA

Gene: ldrA Accession Numbers: G0-9602 (EcoCyc), b4419, ECK1204

Regulation Summary Diagram: ?

LdrA is the toxin of a type I toxin-antitoxin system. Expression of LdrA inhibits ATP synthesis, which may lead to the observed inhibitory effect on DNA replication, transcription and translation [Yamaguchi14].

LdrA is part of one of a set of four Long Direct Repeats (LDRs) in the E. coli genome. ldrA, ldrB and ldrC are clustered together in tandem repeats, with ldrD elsewhere on the genome [Kawano02a].

Deletions of LDR-ABC, LDR-D and LDR-ABCD have no effect under laboratory conditions [Kawano02]. Overexpression of LdrA is toxic; C-terminally tagged LdrA retains its toxic effect, while N-terminally tagged LdrA is no longer toxic [Yamaguchi14].

Reviews: [Gerdes07, Alix09, Kawano12]

Locations: inner membrane

Map Position: [1,268,391 <- 1,268,498] (27.34 centisomes)
Length: 108 bp / 35 aa

Molecular Weight of Polypeptide: 4.014 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0047280 , EchoBASE:EB4335 , EcoGene:EG14403 , EcoliWiki:b4419 , PortEco:ldrA , PR:PRO_000023077 , RefSeq:YP_025297 , RegulonDB:G0-9602

Relationship Links: UniProt:PART-OF:Q6BF86

GO Terms:

Biological Process: GO:0008219 - cell death Inferred from experiment [Yamaguchi14]
GO:0012501 - programmed cell death Inferred by computational analysis [Kawano02]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment [Yamaguchi14]

MultiFun Terms: cell processes protection cell killing

Curated 07-Feb-2006 by Shearer A , SRI International
Last-Curated ? 18-Feb-2014 by Keseler I , SRI International

Gene Local Context (not to scale): ?

Transcription Unit:



Alix09: Alix E, Blanc-Potard AB (2009). "Hydrophobic peptides: novel regulators within bacterial membrane." Mol Microbiol 72(1);5-11. PMID: 19210615

Gerdes07: Gerdes K, Wagner EG (2007). "RNA antitoxins." Curr Opin Microbiol 10(2);117-24. PMID: 17376733

Kawano02: Kawano M, Oshima T, Kasai H, Mori H (2002). "Molecular characterization of long direct repeat (LDR) sequences expressing a stable mRNA encoding for a 35-amino-acid cell-killing peptide and a cis-encoded small antisense RNA in Escherichia coli." Mol Microbiol 45(2);333-49. PMID: 12123448

Kawano02a: Kawano M, Kanaya S, Oshima T, Masuda Y, Ara T, Mori H (2002). "Distribution of repetitive sequences on the leading and lagging strands of the Escherichia coli genome: comparative study of Long Direct Repeat (LDR) sequences." DNA Res 9(1);1-10. PMID: 11939563

Kawano12: Kawano M (2012). "Divergently overlapping cis-encoded antisense RNA regulating toxin-antitoxin systems from E. coli: hok/sok, ldr/rdl, symE/symR." RNA Biol 9(12);1520-7. PMID: 23131729

Yamaguchi14: Yamaguchi Y, Tokunaga N, Inouye M, Phadtare S (2014). "Characterization of LdrA (Long Direct Repeat A) Protein of Escherichia coli." J Mol Microbiol Biotechnol 24(2);91-97. PMID: 24513967

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Mar 5, 2015, BIOCYC13B.