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Escherichia coli K-12 substr. MG1655 Polypeptide: entericidin B



Gene: ecnB Accession Numbers: G0-9562 (EcoCyc), b4411, ECK4143

Synonyms: yjeU

Regulation Summary Diagram: ?

Component of: entericidin toxin-antitoxin complex (extended summary available)

Summary:
EcnAB form a linked toxin-antitoxin "addiction module" which functions to regulate programmed bacterial cell death under high osmolarity conditions. The entericidin A lipoprotein (EcnA) functions as an antidote to the bacteriolytic entericidin B lipoprotein [Bishop98].

Locations: inner membrane

Map Position: [4,374,576 -> 4,374,722] (94.29 centisomes)
Length: 147 bp / 48 aa

Molecular Weight of Polypeptide: 4.81 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0047211 , EchoBASE:EB4091 , EcoGene:EG14345 , EcoliWiki:b4411 , PortEco:ecnB , Pride:P0ADB7 , Protein Model Portal:P0ADB7 , RefSeq:YP_026284 , RegulonDB:G0-9562 , String:511145.b4411 , UniProt:P0ADB7

Relationship Links: InterPro:IN-FAMILY:IPR012556 , Pfam:IN-FAMILY:PF08085 , Prosite:IN-FAMILY:PS51257

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0009636 - response to toxic substance Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, Bishop98]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: cell processes protection cell killing

Essentiality data for ecnB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of: entericidin toxin-antitoxin complex

Subunit composition of entericidin toxin-antitoxin complex = [EcnB][EcnA]
         entericidin B = EcnB (summary available)
         entericidin A, antidote to lipoprotein entericidin B = EcnA (summary available)

Summary:
The entericidin locus of Escherichia coli encodes tandem paralagous genes (ecnAB) which express two small cell-membrane associated lipoproteins [Bishop98]: EcnA and EcnB. EcnAB form a linked toxin-antitoxin "addiction module" which functions to regulate programmed bacterial cell death under high osmolarity conditions. The entericidin A lipoprotein (EcnA) functions as an antidote to the bacteriolytic entericidin B lipoprotein [Bishop98]. Subcloning the ecnAB locus into an inducible plasmid vector resulted in a stationary-phase bacteriolysis phenotype which was shown to be governed by an osmotically active sigma-S promoter [Bishop98]. The rate of stationary-phase bacteriolysis was highest when ecnB was expressed independently of ecnA whereas stationary-phase bacteriolysis was suppressed when ecnA was expressed in cis to ecnB, suggesting that EcnA could serve as an antidote to the bacteriolytic action of EcnB [Bishop98]. Taken together, the experimental results indicate that the chromosomal entericidin locus could regulate programmed bacterial cell death if a low rate of bacteriolysis supports the overall well-being of a healthy sub-population.


Gene Local Context (not to scale): ?

Transcription Units:

Notes:


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bishop98: Bishop RE, Leskiw BK, Hodges RS, Kay CM, Weiner JH (1998). "The entericidin locus of Escherichia coli and its implications for programmed bacterial cell death." J Mol Biol 1998;280(4);583-96. PMID: 9677290

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Gogol11: Gogol EB, Rhodius VA, Papenfort K, Vogel J, Gross CA (2011). "Small RNAs endow a transcriptional activator with essential repressor functions for single-tier control of a global stress regulon." Proc Natl Acad Sci U S A 108(31);12875-80. PMID: 21768388


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc13.