|Gene:||fnrS||Accession Numbers: G0-10677 (EcoCyc), b4699, ECK4511|
Superclasses: a regulatory RNA
FnrS is a small regulatory RNA that reprograms metabolism in response to anaerobiosis. Potential regulatory targets were identified by gene expression analysis and proteomic profiling, and selected targets were validated [Durand10, Boysen10]. Additional targets of FnrS have been identified by comparative genomics [Wright13].
Hfq interacts with and stabilizes FnrS; in an hfq mutant strain, the half life of FnrS is decreased 15-fold from 100 minutes in the wild-type background [Boysen10].
Expression of FnrS is occurs only under anaerobic growth conditions and is dependent on FNR [Durand10, Boysen10] and affected by ArcA and CRP [Durand10]. When the existence of FnrS (RydA) was first proposed, no expression could be detected on Northern blots [Wassarman01].
FnrS: "FNR-regulated sRNA" [Boysen10]
|Map Position: [1,407,153 -> 1,407,271] (30.33 centisomes, 109°)||Length: 119 bp|
|Biological Process:||GO:0040033 - negative regulation of translation, ncRNA-mediated [Boysen10]|
|Molecular Function:||GO:0000499 - base pairing with mRNA
GO:0005515 - protein binding [Boysen10]
|MultiFun Terms:||information transfer → RNA related → antisense RNA|
|regulation → type of regulation → posttranscriptional → antisense RNA|
Boysen10: Boysen A, Moller-Jensen J, Kallipolitis B, Valentin-Hansen P, Overgaard M (2010). "Translational regulation of gene expression by an anaerobically induced small non-coding RNA in Escherichia coli." J Biol Chem 285(14);10690-702. PMID: 20075074
Wright13: Wright PR, Richter AS, Papenfort K, Mann M, Vogel J, Hess WR, Backofen R, Georg J (2013). "Comparative genomics boosts target prediction for bacterial small RNAs." Proc Natl Acad Sci U S A 110(37);E3487-96. PMID: 23980183
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