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Escherichia coli K-12 substr. MG1655 RNA: FnrS small regulatory RNA



Gene: fnrS Accession Numbers: G0-10677 (EcoCyc), b4699, ECK4511

Synonyms: rydA

Superclasses: a regulatory RNA

Regulation Summary Diagram: ?

Summary:
FnrS is a small regulatory RNA that reprograms metabolism in response to anaerobiosis. Potential regulatory targets were identified by gene expression analysis and proteomic profiling, and selected targets were validated [Durand10, Boysen10]. Additional targets of FnrS have been identified by comparative genomics [Wright13].

Hfq interacts with and stabilizes FnrS; in an hfq mutant strain, the half life of FnrS is decreased 15-fold from 100 minutes in the wild-type background [Boysen10].

Expression of FnrS is occurs only under anaerobic growth conditions and is dependent on FNR [Durand10, Boysen10] and affected by ArcA and CRP [Durand10]. When the existence of FnrS (RydA) was first proposed, no expression could be detected on Northern blots [Wassarman01].

FnrS: "FNR-regulated sRNA" [Boysen10]

Map Position: [1,407,153 -> 1,407,271] (30.33 centisomes)
Length: 119 bp

Genetic Regulation Schematic: ?

Unification Links: EcoGene:EG31185 , PortEco:fnrS

GO Terms:

Biological Process: GO:0040033 - negative regulation of translation, ncRNA-mediated Inferred from experiment [Boysen10]
Molecular Function: GO:0000499 - base pairing with mRNA Inferred from experiment [Durand10]
GO:0005515 - protein binding Inferred from experiment [Boysen10]

MultiFun Terms: information transfer RNA related antisense RNA
regulation type of regulation posttranscriptional antisense RNA

Regulated Transcription Units (11 total): ?

Notes:


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

Credits:
Created 14-Jan-2010 by Keseler I , SRI International
Last-Curated ? 14-Nov-2013 by Keseler I , SRI International


References

Boysen10: Boysen A, Moller-Jensen J, Kallipolitis B, Valentin-Hansen P, Overgaard M (2010). "Translational regulation of gene expression by an anaerobically induced small non-coding RNA in Escherichia coli." J Biol Chem 285(14);10690-702. PMID: 20075074

Durand10: Durand S, Storz G (2010). "Reprogramming of anaerobic metabolism by the FnrS small RNA." Mol Microbiol 75(5);1215-31. PMID: 20070527

Wassarman01: Wassarman KM, Repoila F, Rosenow C, Storz G, Gottesman S (2001). "Identification of novel small RNAs using comparative genomics and microarrays." Genes Dev 15(13);1637-51. PMID: 11445539

Wright13: Wright PR, Richter AS, Papenfort K, Mann M, Vogel J, Hess WR, Backofen R, Georg J (2013). "Comparative genomics boosts target prediction for bacterial small RNAs." Proc Natl Acad Sci U S A 110(37);E3487-96. PMID: 23980183


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC14A.