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Escherichia coli K-12 substr. MG1655 Polypeptide: molybdate ABC transporter - periplasmic binding protein



Gene: modA Accession Numbers: EG12427 (EcoCyc), b0763, ECK0752

Regulation Summary Diagram: ?

Component of: molybdate ABC transporter (extended summary available)

Summary:
ModA is the periplasmic binding component of the molybdate ABC transporter. The promoter of modA is predicted to be σ28-dependent and can be transcribed by RNA polymerase sigma 28 in vitro [Yu06b].

Gene Citations: [Grunden96, Rech96, McNicholas97]

Locations: periplasmic space

Map Position: [794,312 -> 795,085] (17.12 centisomes)
Length: 774 bp / 257 aa

Molecular Weight of Polypeptide: 27.364 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002601 , CGSC:37372 , EchoBASE:EB2325 , EcoGene:EG12427 , EcoliWiki:b0763 , ModBase:P37329 , OU-Microarray:b0763 , PortEco:modA , PR:PRO_000023267 , Pride:P37329 , Protein Model Portal:P37329 , RefSeq:NP_415284 , RegulonDB:EG12427 , SMR:P37329 , String:511145.b0763 , UniProt:P37329

Relationship Links: InterPro:IN-FAMILY:IPR005950 , PDB:Structure:1AMF , PDB:Structure:1WOD , PDB:Structure:3AXF , PDB:Structure:3R26 , Pfam:IN-FAMILY:PF01547

In Paralogous Gene Group: 216 (4 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0015689 - molybdate ion transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0015412 - molybdate transmembrane-transporting ATPase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [GOA01a, DiazMejia09, Han13, LopezCampistrou05]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: metabolism biosynthesis of building blocks cofactors, small molecule carriers molybdenum
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, periplasmic binding component

Essentiality data for modA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: molybdate ABC transporter

Subunit composition of molybdate ABC transporter = [ModC]2[ModB]2[ModA]
         molybdate ABC transporter - ATP binding subunit = ModC (summary available)
         molybdate ABC transporter - membrane subunit = ModB (summary available)
         molybdate ABC transporter - periplasmic binding protein = ModA (summary available)

Summary:
Molybdate is important for the production of several molybdoenzymes in E. coli growing under anaerobic conditions. ModCBA is a high-affinity molybdate transporter that is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95] and was identified based on its ability to complement a tor mutation [Miyake95a]. Based on sequence similarity, modA encodes the periplasmic binding component, modB encodes the integral membrane component, and modC encodes the ATP-binding component of the ABC transporter. Complementation of a mod mutant with the cloned mod genes restored molybdate uptake activities [Walkenhorst95]. In a mod mutant, molybdate is not transported by the ModCBA system but by the sulfate ABC transporter or by a nonspecific anion transporter [Grunden97, Rosentel95]. ModA specifically binds molybdate and tungstate but not sulfate [Dudev04]. ModA binds molybdate and tungstate in a 1:1 ratio [Imperial98]. The structures of ModA complexed with molybdate and tungstate have been determined to 1.75 Å resolutions [Hu97a].

Transcription of mod genes is regulated by a molybdate-dependent repressor protein, ModE [Grunden97, Grunden96], requiring two ModE-molybdate molecules per ModA operator [Grunden99].

Review: [Grunden97]

Citations: [Rech95, MaupinFurlow95, McNicholas96, McNicholas97]


Enzymatic reaction of: transport of molybdate (molybdate ABC transporter)

EC Number: 3.6.3.29

Kinetic Parameters:

Substrate
Km (μM)
Citations
molybdate
0.045
[Grunden97, BRENDA14]


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 24
[Rech96]
 
Chain 25 -> 257
[UniProt09]
UniProt: Molybdate-binding periplasmic protein;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b0763 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12427; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dudev04: Dudev T, Lim C (2004). "Oxyanion selectivity in sulfate and molybdate transport proteins: an ab initio/CDM study." J Am Chem Soc 126(33);10296-305. PMID: 15315443

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Grunden96: Grunden AM, Ray RM, Rosentel JK, Healy FG, Shanmugam KT (1996). "Repression of the Escherichia coli modABCD (molybdate transport) operon by ModE." J Bacteriol 1996;178(3);735-44. PMID: 8550508

Grunden97: Grunden AM, Shanmugam KT (1997). "Molybdate transport and regulation in bacteria." Arch Microbiol 1997;168(5);345-54. PMID: 9325422

Grunden99: Grunden AM, Self WT, Villain M, Blalock JE, Shanmugam KT (1999). "An analysis of the binding of repressor protein ModE to modABCD (molybdate transport) operator/promoter DNA of Escherichia coli." J Biol Chem 1999;274(34);24308-15. PMID: 10446207

Han13: Han MJ, Kim JY, Kim JA (2013). "Comparison of the large-scale periplasmic proteomes of the Escherichia coli K-12 and B strains." J Biosci Bioeng. PMID: 24140104

Hu97a: Hu Y, Rech S, Gunsalus RP, Rees DC (1997). "Crystal structure of the molybdate binding protein ModA." Nat Struct Biol 4(9);703-7. PMID: 9302996

Imperial98: Imperial J, Hadi M, Amy NK (1998). "Molybdate binding by ModA, the periplasmic component of the Escherichia coli mod molybdate transport system." Biochim Biophys Acta 1370(2);337-46. PMID: 9545596

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

MaupinFurlow95: Maupin-Furlow JA, Rosentel JK, Lee JH, Deppenmeier U, Gunsalus RP, Shanmugam KT (1995). "Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli." J Bacteriol 177(17);4851-6. PMID: 7665460

McNicholas96: McNicholas PM, Chiang RC, Gunsalus RP (1996). "The Escherichia coli modE gene: effect of modE mutations on molybdate dependent modA expression." FEMS Microbiol Lett 145(1);117-23. PMID: 8931336

McNicholas97: McNicholas PM, Rech SA, Gunsalus RP (1997). "Characterization of the ModE DNA-binding sites in the control regions of modABCD and moaABCDE of Escherichia coli." Mol Microbiol 1997;23(3);515-24. PMID: 9044285

Miyake95a: Miyake H, Yabu H, Satoh T, Yamamoto I (1995). "Characterization and transcriptional regulation of the modABCD genes for molybdenum transport in Escherichia coli." Nucleic Acids Symp Ser (34);91-2. PMID: 8841567

Rech95: Rech S, Deppenmeier U, Gunsalus RP (1995). "Regulation of the molybdate transport operon, modABCD, of Escherichia coli in response to molybdate availability." J Bacteriol 177(4);1023-9. PMID: 7860583

Rech96: Rech S, Wolin C, Gunsalus RP (1996). "Properties of the periplasmic ModA molybdate-binding protein of Escherichia coli." J Biol Chem 1996;271(5);2557-62. PMID: 8576221

Rosentel95: Rosentel JK, Healy F, Maupin-Furlow JA, Lee JH, Shanmugam KT (1995). "Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli." J Bacteriol 1995;177(17);4857-64. PMID: 7665461

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Walkenhorst95: Walkenhorst HM, Hemschemeier SK, Eichenlaub R (1995). "Molecular analysis of the molybdate uptake operon, modABCD, of Escherichia coli and modR, a regulatory gene." Microbiol Res 1995;150(4);347-61. PMID: 8564363

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Yu06b: Yu HH, Kibler D, Tan M (2006). "In Silico Prediction and Functional Validation of {sigma}28-Regulated Genes in Chlamydia and Escherichia coli." J Bacteriol 188(23);8206-8212. PMID: 16997971

Other References Related to Gene Regulation

Park01: Park K, Choi S, Ko M, Park C (2001). "Novel sigmaF-dependent genes of Escherichia coli found using a specified promoter consensus." FEMS Microbiol Lett 2001;202(2);243-50. PMID: 11520622

Yu06a: Yu HH, Di Russo EG, Rounds MA, Tan M (2006). "Mutational analysis of the promoter recognized by Chlamydia and Escherichia coli sigma(28) RNA polymerase." J Bacteriol 188(15);5524-31. PMID: 16855242

Zhao07: Zhao K, Liu M, Burgess RR (2007). "Adaptation in bacterial flagellar and motility systems: from regulon members to 'foraging'-like behavior in E. coli." Nucleic Acids Res 35(13);4441-52. PMID: 17576668

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Tue Nov 25, 2014, biocyc13.