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Escherichia coli K-12 substr. MG1655 Polypeptide: xylose ABC transporter - ATP binding subunit



Gene: xylG Accession Numbers: EG12275 (EcoCyc), b3567, ECK3556

Regulation Summary Diagram: ?

Component of: xylose ABC transporter (extended summary available)

Summary:
XylG is the ATP-binding component of the XylFGH xylose ABC transporter.

Gene Citations: [Sumiya95, Song97]

Locations: inner membrane, cytosol

Map Position: [3,730,224 -> 3,731,765] (80.4 centisomes)
Length: 1542 bp / 513 aa

Molecular Weight of Polypeptide: 56.47 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0011648 , CGSC:33780 , EchoBASE:EB2184 , EcoGene:EG12275 , EcoliWiki:b3567 , ModBase:P37388 , OU-Microarray:b3567 , PortEco:xylG , PR:PRO_000024243 , Pride:P37388 , Protein Model Portal:P37388 , RefSeq:NP_418024 , RegulonDB:EG12275 , SMR:P37388 , String:511145.b3567 , UniProt:P37388

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR013455 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Prosite:IN-FAMILY:PS51280 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0015752 - D-ribose transport Inferred from experiment [Song98]
GO:0015753 - D-xylose transport Inferred from experiment Inferred by computational analysis [GOA06, GOA01a, Sumiya95]
GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0015611 - D-ribose-importing ATPase activity Inferred from experiment [Song98]
GO:0015614 - D-xylose-importing ATPase activity Inferred from experiment Inferred by computational analysis [GOA06, GOA01a, Sumiya95]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA06, GOA01a]
GO:0015407 - monosaccharide-transporting ATPase activity Inferred by computational analysis [GOA01]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [GOA06]

MultiFun Terms: metabolism carbon utilization carbon compounds
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for xylG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: xylose ABC transporter

Synonyms: XylFGH

Subunit composition of xylose ABC transporter = [XylG]2[XylH]2[XylF]
         xylose ABC transporter - ATP binding subunit = XylG (summary available)
         xylose ABC transporter - membrane subunit = XylH (summary available)
         xylose ABC transporter - periplasmic binding protein = XylF (summary available)

Summary:
XylFGH is a D-xylose transporter belonging to the ATP Binding Cassette (ABC) Superfamily [Wu95]. D-xylose is the most abundant sugar in nature after glucose, and it can be utilized by E. coli as a sole carbon source and metabolized through the pentose phosphate pathway [Song98]. Sequence homology analysis suggests that XylG is the ATP-binding protein, XylF is the periplasmic substrate-binding protein [Sumiya95], and XylH is the membrane component of the ABC transport system [Sofia94]. The binding affinity of the receptor protein, XylF, was studied using a mutant of xylE, which encodes the other D-Xylose transport system in E. coli. The Km of XylF was determined to be in the range of 0.2-4 μM, indicating that XylF is a high-affinity D-xylose transport system [Sumiya95]. Transposon mutagenesis studies suggest that XylFGH can also transport D-ribose in E.coli cells lacking the high affinity RbsABC ribose transport system [Song98].


Enzymatic reaction of: transport of D-xylose (xylose ABC transporter)

EC Number: 3.6.3.17

Alternative Products for D-xylose: β-D-ribopyranose [Song98 ]


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 5 -> 242
[UniProt09]
UniProt: ABC transporter 1;
Nucleotide-Phosphate-Binding-Region 37 -> 44
[UniProt10a]
UniProt: ATP; Non-Experimental Qualifier: potential;
Conserved-Region 259 -> 505
[UniProt09]
UniProt: ABC transporter 2;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b3567 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12275; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Sofia94: Sofia HJ, Burland V, Daniels DL, Plunkett G, Blattner FR (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 1994;22(13);2576-86. PMID: 8041620

Song97: Song S, Park C (1997). "Organization and regulation of the D-xylose operons in Escherichia coli K-12: XylR acts as a transcriptional activator." J Bacteriol 1997;179(22);7025-32. PMID: 9371449

Song98: Song S, Park C (1998). "Utilization of D-ribose through D-xylose transporter." FEMS Microbiol Lett 1998;163(2);255-61. PMID: 9673030

Sumiya95: Sumiya M, Davis EO, Packman LC, McDonald TP, Henderson PJ (1995). "Molecular genetics of a receptor protein for D-xylose, encoded by the gene xylF, in Escherichia coli." Receptors Channels 1995;3(2);117-28. PMID: 8581399

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Other References Related to Gene Regulation

Beisel11: Beisel CL, Storz G (2011). "The base-pairing RNA spot 42 participates in a multioutput feedforward loop to help enact catabolite repression in Escherichia coli." Mol Cell 41(3);286-97. PMID: 21292161

Bradley07: Bradley MD, Beach MB, de Koning AP, Pratt TS, Osuna R (2007). "Effects of Fis on Escherichia coli gene expression during different growth stages." Microbiology 153(Pt 9);2922-40. PMID: 17768236

Laikova01: Laikova ON, Mironov AA, Gelfand MS (2001). "Computational analysis of the transcriptional regulation of pentose utilization systems in the gamma subdivision of Proteobacteria." FEMS Microbiol Lett 205(2);315-22. PMID: 11750821

Xu95e: Xu J, Johnson RC (1995). "Identification of genes negatively regulated by Fis: Fis and RpoS comodulate growth-phase-dependent gene expression in Escherichia coli." J Bacteriol 177(4);938-47. PMID: 7860604


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC14B.