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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted inner membrane protein



Gene: bcsG Accession Numbers: EG12265 (EcoCyc), b3538, ECK3523

Synonyms: yhjU

Regulation Summary Diagram: ?

Summary:
The homolog of bcsG in natural isolates of Salmonella enteritidis has been shown to be required for cellulose production and contributing to rigid pellicle and biofilm formation [Solano02].

In E. coli K-12 strains such as W3110 and MG1655, a "domesticating SNP" consists of a point mutation that introduces a stop codon after the first 5 amino acids of the wild-type bcsQ ORF. The bcsQ ORF shown in EcoCyc begins just downstream of this SNP and appears to be non-functional. This domesticating SNP lowers expression of both bcsQ and the downstream bcsA gene. After repairing the SNP, the resulting "de-domesticated" W3110 derivative strain produces cellulose and has dramatically altered biofilm morphology. A ΔbcsEFG mutation introduced into this strain leads to significantly reduced cellulose biosynthesis [Serra13].

BcsG: "bacterial cellulose synthesis"

Locations: inner membrane

Map Position: [3,696,237 -> 3,697,916] (79.67 centisomes)
Length: 1680 bp / 559 aa

Molecular Weight of Polypeptide: 62.032 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0011558 , DIP:DIP-12392N , EchoBASE:EB2174 , EcoGene:EG12265 , EcoliWiki:b3538 , Mint:MINT-1289882 , OU-Microarray:b3538 , PortEco:bcsG , Protein Model Portal:P37659 , RefSeq:NP_417995 , RegulonDB:EG12265 , String:511145.b3538 , UniProt:P37659

Relationship Links: InterPro:IN-FAMILY:IPR017744 , Pfam:IN-FAMILY:PF11658

GO Terms:

Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

Gene Class: ORFs

Essentiality data for bcsG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 24-Jun-2014 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 34 -> 54
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 68 -> 88
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 113 -> 133
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 138 -> 158
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: potential.


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b3538 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12265; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Serra13: Serra DO, Richter AM, Hengge R (2013). "Cellulose as an architectural element in spatially structured Escherichia coli biofilms." J Bacteriol 195(24);5540-54. PMID: 24097954

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Solano02: Solano C, Garcia B, Valle J, Berasain C, Ghigo JM, Gamazo C, Lasa I (2002). "Genetic analysis of Salmonella enteritidis biofilm formation: critical role of cellulose." Mol Microbiol 43(3);793-808. PMID: 11929533

UniProt12a: UniProt Consortium (2012). "UniProt version 2012-09 released on 2012-09-12 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, BIOCYC13B.