Escherichia coli K-12 substr. MG1655 Protein: copper binding protein CutA

Gene: cutA Accession Numbers: EG12177 (EcoCyc), b4137, ECK4131

Synonyms: cutA1

Regulation Summary Diagram: ?

Regulation summary diagram for cutA

Subunit composition of copper binding protein CutA = [CutA]3
         copper binding protein CutA = CutA

CutA is a copper-binding protein that is also required for copper tolerance.

Mutants lacking CutA function show increased copper sensitivity and uptake. CutA is also involved in tolerance of zinc, nickel, cobalt and cadium. It is induced by the presence of copper, zinc, nickel and cobalt, and to a lesser extent cadmium, manganese and silver [Fong95].

The crystal structure of CutA has been determined to 1.7 Å resolution. It forms a trimer [Arnesano03].

CutA can bind copper in vitro [Arnesano03].

Gene Citations: [Crooke95]

Locations: cytosol

Map Position: [4,363,041 <- 4,363,379] (94.04 centisomes, 339°)
Length: 339 bp / 112 aa

Molecular Weight of Polypeptide: 12.331 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013544 , CGSC:34216 , DIP:DIP-47984N , EchoBASE:EB2094 , EcoGene:EG12177 , EcoliWiki:b4137 , ModBase:P69488 , OU-Microarray:b4137 , PortEco:cutA , PR:PRO_000022355 , Protein Model Portal:P69488 , RefSeq:NP_418560 , RegulonDB:EG12177 , SMR:P69488 , String:511145.b4137 , UniProt:P69488

Relationship Links: InterPro:IN-FAMILY:IPR004323 , InterPro:IN-FAMILY:IPR011322 , InterPro:IN-FAMILY:IPR023700 , Panther:IN-FAMILY:PTHR23419 , PDB:Structure:1NAQ , PDB:Structure:3AA8 , PDB:Structure:3AA9 , PDB:Structure:3AH6 , Pfam:IN-FAMILY:PF03091

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0046688 - response to copper ion Inferred from experiment [Fong95]
GO:0051260 - protein homooligomerization Inferred from experiment [Arnesano03]
GO:0010038 - response to metal ion Inferred by computational analysis [GOA01]
Molecular Function: GO:0046872 - metal ion binding Inferred from experiment Inferred by computational analysis [UniProtGOA11, Arnesano03]
GO:0005507 - copper ion binding Inferred by computational analysis [GOA06, GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA06, GOA01]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09, Fong95]

MultiFun Terms: cell processes adaptations other (mechanical, nutritional, oxidative stress)

Essentiality data for cutA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Created 31-Jan-2006 by Shearer A , SRI International
Last-Curated ? 31-Jan-2006 by Shearer A , SRI International

Sequence Features

Protein sequence of copper binding protein CutA with features indicated

Feature Class Location Citations Comment
Metal-Binding-Site 16
UniProt: Copper; Non-Experimental Qualifier: probable;
Sequence-Conflict 24
[Crooke95, UniProt10a]
UniProt: (in Ref. 1);
Metal-Binding-Site 83
UniProt: Copper; Non-Experimental Qualifier: probable;
Metal-Binding-Site 84
UniProt: Copper; Non-Experimental Qualifier: probable;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b4137 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12177; confirmed by SwissProt match.


Arnesano03: Arnesano F, Banci L, Benvenuti M, Bertini I, Calderone V, Mangani S, Viezzoli MS (2003). "The evolutionarily conserved trimeric structure of CutA1 proteins suggests a role in signal transduction." J Biol Chem 278(46);45999-6006. PMID: 12949080

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Crooke95: Crooke H, Cole J (1995). "The biogenesis of c-type cytochromes in Escherichia coli requires a membrane-bound protein, DipZ, with a protein disulphide isomerase-like domain." Mol Microbiol 1995;15(6);1139-50. PMID: 7623667

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Fong95: Fong ST, Camakaris J, Lee BT (1995). "Molecular genetics of a chromosomal locus involved in copper tolerance in Escherichia coli K-12." Mol Microbiol 1995;15(6);1127-37. PMID: 7623666

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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