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Escherichia coli K-12 substr. MG1655 Polypeptide: Rac prophage; inhibitor of FtsZ, killing protein



Gene: kilR Accession Numbers: EG12155 (EcoCyc), b1352, ECK1350

Synonyms: ydaD, kil

Regulation Summary Diagram: ?

Summary:
kilR is the kil gene of the defective lambdoid prophage Rac. Transfer of the Rac locus into a rac- strain kills the recipient strain [Feinstein82]. This killing depends on the presence of KilR, whose overexpression inhibits cell division and leads to morphological defects [Conter96, Burke13]. Overexpression of the cell division gene ftsZ can counter the effects of KilR expression [Conter96].

Deletion of kilR reduces cell viability and reduces the colony size after exposure to some antibiotics [Wang10b], but increases resistance to the Rho inhibitor bicyclomycin [Cardinale08, Tran11].

The effects of KilR appear to be positively modulated by CRP-cAMP, though the mechanism by which this occurs is unknown [Conter96]. Expression of kilR is induced by antibiotics such as nalidixic acid and azlocillin [Wang10b].

Locations: cytosol

Map Position: [1,416,032 <- 1,416,253] (30.52 centisomes)
Length: 222 bp / 73 aa

Molecular Weight of Polypeptide: 8.434 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004537 , EchoBASE:EB2075 , EcoGene:EG12155 , EcoliWiki:b1352 , OU-Microarray:b1352 , PortEco:kilR , PR:PRO_000023066 , Protein Model Portal:P38393 , RefSeq:NP_415869 , RegulonDB:EG12155 , String:511145.b1352 , UniProt:P38393

GO Terms:

Biological Process: GO:0046677 - response to antibiotic Inferred from experiment [Wang10b]
GO:0051782 - negative regulation of cell division Inferred from experiment [Conter96]
GO:0007049 - cell cycle Inferred by computational analysis [UniProtGOA11a]
GO:0051301 - cell division Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: extrachromosomal prophage genes and phage related functions

Essentiality data for kilR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Curated 28-Apr-2006 by Shearer A , SRI International
Last-Curated ? 16-Apr-2013 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Extrinsic-Sequence-Variant 51
[UniProt10]
Alternate sequence: F → C; UniProt: (in strain: B);


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
10/20/97 Gene b1352 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12155; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Burke13: Burke C, Liu M, Britton W, Triccas JA, Thomas T, Smith AL, Allen S, Salomon R, Harry E (2013). "Harnessing single cell sorting to identify cell division genes and regulators in bacteria." PLoS One 8(4);e60964. PMID: 23565292

Cardinale08: Cardinale CJ, Washburn RS, Tadigotla VR, Brown LM, Gottesman ME, Nudler E (2008). "Termination factor Rho and its cofactors NusA and NusG silence foreign DNA in E. coli." Science 320(5878);935-8. PMID: 18487194

Conter96: Conter A, Bouche JP, Dassain M (1996). "Identification of a new inhibitor of essential division gene ftsZ as the kil gene of defective prophage Rac." J Bacteriol 178(17);5100-4. PMID: 8752325

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feinstein82: Feinstein SI, Low KB (1982). "Zygotic induction of the rac locus can cause cell death in E. coli." Mol Gen Genet 187(2);231-5. PMID: 6217397

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Tran11: Tran L, van Baarsel JA, Washburn RS, Gottesman ME, Miller JH (2011). "Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho." J Bacteriol 193(9);2229-35. PMID: 21357484

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wang10b: Wang X, Kim Y, Ma Q, Hong SH, Pokusaeva K, Sturino JM, Wood TK (2010). "Cryptic prophages help bacteria cope with adverse environments." Nat Commun 1(9);147. PMID: 21266997


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, BIOCYC14B.