Escherichia coli K-12 substr. MG1655 Polypeptide: predicted autotransporter outer membrane protein

Gene: yejO Accession Numbers: EG12051 (EcoCyc), b2190, ECK2184

Regulation Summary Diagram: ?

Regulation summary diagram for yejO

YejO shows sequence similarity to the α43 subunit of Ag43, which is the outer membrane protein responsible for phase-variable auto-aggregation in E. coli [Henderson99]. The function of YejO is unknown.

Locations: extracellular space, outer membrane

Map Position: [2,284,412 <- 2,286,922] (49.24 centisomes, 177°)
Length: 2511 bp / 836 aa

Molecular Weight of Polypeptide: 88.476 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007244 , DIP:DIP-11942N , EchoBASE:EB1982 , EcoGene:EG12051 , EcoliWiki:b2190 , Mint:MINT-1322746 , ModBase:P33924 , OU-Microarray:b2190 , PortEco:yejO , Pride:P33924 , Protein Model Portal:P33924 , RefSeq:NP_416694 , RegulonDB:EG12051 , SMR:P33924 , String:316407.yejO , Swiss-Model:P33924 , UniProt:P33924

Relationship Links: InterPro:IN-FAMILY:IPR003991 , InterPro:IN-FAMILY:IPR004899 , InterPro:IN-FAMILY:IPR005546 , InterPro:IN-FAMILY:IPR006315 , InterPro:IN-FAMILY:IPR011050 , InterPro:IN-FAMILY:IPR012332 , Pfam:IN-FAMILY:PF03212 , Pfam:IN-FAMILY:PF03797 , Prints:IN-FAMILY:PR01484 , Prosite:IN-FAMILY:PS51208 , Smart:IN-FAMILY:SM00869

In Paralogous Gene Group: 387 (2 members) , 388 (2 members)

GO Terms:

Biological Process: GO:0007155 - cell adhesion Inferred by computational analysis [GOA01]
Cellular Component: GO:0005576 - extracellular region Inferred by computational analysis [DiazMejia09]
GO:0009279 - cell outer membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]
GO:0019867 - outer membrane Inferred by computational analysis [GOA01]

Gene Class: ORFs

Essentiality data for yejO knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b2190 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12051; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Henderson99: Henderson IR, Owen P (1999). "The major phase-variable outer membrane protein of Escherichia coli structurally resembles the immunoglobulin A1 protease class of exported protein and is regulated by a novel mechanism involving Dam and oxyR." J Bacteriol 181(7);2132-41. PMID: 10094691

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Stafford05: Stafford GP, Ogi T, Hughes C (2005). "Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2." Microbiology 151(Pt 6);1779-88. PMID: 15941987

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc13.