Escherichia coli K-12 substr. MG1655 Enzyme: fructose 6-phosphate aldolase 2

Gene: fsaB Accession Numbers: EG11905 (EcoCyc), b3946, ECK3938

Synonyms: yijG, talC, FSA 2

Regulation Summary Diagram: ?

Regulation summary diagram for fsaB

Fructose 6-phosphate aldolase (FSA) is a novel enzyme activity. Its discovery in E. coli appears to be the first report of this activity in any organism. The fsaB (formerly talC, [Reizer95]) gene encodes a second FSA in E. coli whose activity has not been studied in detail. The enzyme is a class I aldolase. The physiological role of FSA is not yet known [Schurmann01].

FsaB: "fructose-six-phosphate aldolase B"

Locations: cytosol

Map Position: [4,137,069 <- 4,137,731] (89.17 centisomes, 321°)
Length: 663 bp / 220 aa

Molecular Weight of Polypeptide: 23.555 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012915 , EchoBASE:EB1850 , EcoGene:EG11905 , EcoliWiki:b3946 , ModBase:P32669 , OU-Microarray:b3946 , PortEco:fsaB , PR:PRO_000022710 , Protein Model Portal:P32669 , RefSeq:NP_418381 , RegulonDB:EG11905 , SMR:P32669 , String:511145.b3946 , Swiss-Model:P32669 , UniProt:P32669

Relationship Links: InterPro:IN-FAMILY:IPR001585 , InterPro:IN-FAMILY:IPR004731 , InterPro:IN-FAMILY:IPR013785 , InterPro:IN-FAMILY:IPR018225 , InterPro:IN-FAMILY:IPR023001 , Panther:IN-FAMILY:PTHR10683 , Pfam:IN-FAMILY:PF00923 , Prosite:IN-FAMILY:PS00958 , Prosite:IN-FAMILY:PS01054

In Paralogous Gene Group: 2 (3 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0005975 - carbohydrate metabolic process Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0006000 - fructose metabolic process Inferred by computational analysis [GOA06]
Molecular Function: GO:0097023 - fructose 6-phosphate aldolase activity Inferred from experiment [Schurmann01]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0016829 - lyase activity Inferred by computational analysis [UniProtGOA11]
GO:0016832 - aldehyde-lyase activity Inferred by computational analysis [GOA06, GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA06]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism

Essentiality data for fsaB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Last-Curated ? 23-Mar-2011 by Keseler I , SRI International

Enzymatic reaction of: fructose 6-phosphate aldolase

EC Number: 4.1.2.-

β-D-fructofuranose 6-phosphate <=> dihydroxyacetone + D-glyceraldehyde 3-phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Sequence Features

Protein sequence of fructose 6-phosphate aldolase 2 with features indicated

Feature Class Location Citations Comment
Active-Site 85
UniProt: Non-Experimental Qualifier: by similarity;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b3946 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11905; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Reizer95: Reizer J, Reizer A, Saier MH (1995). "Novel phosphotransferase system genes revealed by bacterial genome analysis--a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system." Microbiology 141 ( Pt 4);961-71. PMID: 7773398

Schurmann01: Schurmann M, Sprenger GA (2001). "Fructose-6-phosphate aldolase is a novel class I aldolase from Escherichia coli and is related to a novel group of bacterial transaldolases." J Biol Chem 2001;276(14);11055-61. PMID: 11120740

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Mon Oct 5, 2015, biocyc13.