Escherichia coli K-12 substr. MG1655 Polypeptide: predicted PTS permease - FrvA subunit

Gene: frvA Accession Numbers: EG11864 (EcoCyc), b3900, ECK3893

Synonyms: yiiK

Regulation Summary Diagram: ?

Regulation summary diagram for frvA

Component of: predicted PTS permease FrvAB (summary available)

frvA is predicted to encode a hydrophilic protein with similarity to the IIA domain of the PTS Enzyme IIs specific for mannitol and fructose. FrvA contains a conserved histidine (His64) [Reizer94a, Plunkett93].

Locations: cytosol, membrane

Map Position: [4,090,400 <- 4,090,846] (88.16 centisomes, 317°)
Length: 447 bp / 148 aa

Molecular Weight of Polypeptide: 16.093 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012727 , EchoBASE:EB1810 , EcoGene:EG11864 , EcoliWiki:b3900 , ModBase:P32155 , OU-Microarray:b3900 , PortEco:frvA , PR:PRO_000022703 , Protein Model Portal:P32155 , RefSeq:NP_418336 , RegulonDB:EG11864 , SMR:P32155 , String:511145.b3900 , UniProt:P32155

Relationship Links: InterPro:IN-FAMILY:IPR002178 , InterPro:IN-FAMILY:IPR004715 , InterPro:IN-FAMILY:IPR016152 , Pfam:IN-FAMILY:PF00359 , Prosite:IN-FAMILY:PS00372 , Prosite:IN-FAMILY:PS51094

In Paralogous Gene Group: 187 (14 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11, GOA01, Reizer94a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11]
GO:0034219 - carbohydrate transmembrane transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
GO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred by computational analysis [GOA01]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]
GO:0016021 - integral component of membrane Inferred by computational analysis [GOA01]

MultiFun Terms: metabolism carbon utilization carbon compounds
transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Essentiality data for frvA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: predicted PTS permease FrvAB

Synonyms: EIIFrv, Enzyme IIFrv

Subunit composition of predicted PTS permease FrvAB = [FrvB][FrvA]
         predicted PTS permease - FrvB subunit = FrvB (summary available)
         predicted PTS permease - FrvA subunit = FrvA (summary available)

FrvAB, a predicted PTS permease of unknown specificity, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The PTS transports and simultaneously phosphorylates its sugar substrates in a process called group translocation (reviewed in [Postma93].

Sequence analysis suggests that a predicted FrvAB complex, possesses two domains (IIB-IIC) in a single polypeptide chain (FrvB) and one domain (IIA) in FrvA. It is most similar to the fructose- and mannitol-specific PTS Enzymes II [Reizer94a].

frvAB is part of a gene cluster that includes frvX of unknown function and frvR that may encode a regulatory protein.

Please note that the expression of the frv gene cluster has not been studied and the presence of an frvAB encoded protein complex is hypothetical.

Citations: [Moralejo93, Plunkett93]

Last-Curated ? 04-May-2014 by Mackie A , Macquarie University

Sequence Features

Protein sequence of predicted PTS permease - FrvA subunit with features indicated

Feature Class Location Citations Comment
Conserved-Region 2 -> 145
UniProt: PTS EIIA type-2;
Active-Site 64
UniProt: Tele-phosphohistidine intermediate; Non-Experimental Qualifier: by similarity;
Sequence-Conflict 81
[Plunkett93, UniProt10]
UniProt: (in Ref. 2; AAB03033);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b3900 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11864; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Moralejo93: Moralejo P, Egan SM, Hidalgo E, Aguilar J (1993). "Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli." J Bacteriol 175(17);5585-94. PMID: 8396120

Plunkett93: Plunkett G, Burland V, Daniels DL, Blattner FR (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 1993;21(15);3391-8. PMID: 8346018

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Reizer94a: Reizer J, Michotey V, Reizer A, Saier MH (1994). "Novel phosphotransferase system genes revealed by bacterial genome analysis: unique, putative fructose- and glucoside-specific systems." Protein Sci 3(3);440-50. PMID: 8019415

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Wed Oct 7, 2015, biocyc14.