Escherichia coli K-12 substr. MG1655 Polypeptide: EnvR DNA-binding transcriptional repressor

Gene: envR Accession Numbers: EG11741 (EcoCyc), b3264, ECK3251

Synonyms: acrS, yhdK

Regulation Summary Diagram: ?

Regulation summary diagram for envR

EnvR, also known as AcrS, represses the transcription of genes encoding a drug efflux pump that has a role in resistance to antibiotics [Hirakawa08].

This protein is homologous to some other proteins, such as MtrR of Neisseria gonorrhoeae, TcmR of Streptomyces glaucescens, JadR2 from Streptomyces venezuelae, and AcrR from Escherichia coli [Yang95, Pan94]. All of these proteins, including EnvR, have a helix-turn-helix motif close to the N terminus [Yang95, Pan94].

EnvR recognizes and binds a 24-bp palindromic DNA sequence [Hirakawa08]. The same sequence is also recognized by the transcriptional repressor AcrR [Hirakawa08].

envR is adjacent and divergent in the genome to acrEF, an operon that encodes another drug efflux pump; however, it is not regulated or is only lightly affected by EnvR [Hirakawa08]. Both of these transcription units appears to be repressed by HNS [Hirakawa08].

Locations: cytosol

Map Position: [3,410,825 <- 3,411,487] (73.51 centisomes, 265°)
Length: 663 bp / 220 aa

Molecular Weight of Polypeptide: 25.198 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010712 , EchoBASE:EB1692 , EcoGene:EG11741 , EcoliWiki:b3264 , ModBase:P0ACT2 , OU-Microarray:b3264 , PortEco:envR , Protein Model Portal:P0ACT2 , RefSeq:NP_417730 , RegulonDB:EG11741 , SMR:P0ACT2 , String:511145.b3264 , UniProt:P0ACT2

Relationship Links: InterPro:IN-FAMILY:IPR001647 , InterPro:IN-FAMILY:IPR009057 , InterPro:IN-FAMILY:IPR011075 , InterPro:IN-FAMILY:IPR013572 , InterPro:IN-FAMILY:IPR015893 , InterPro:IN-FAMILY:IPR023772 , Pfam:IN-FAMILY:PF00440 , Pfam:IN-FAMILY:PF08361 , Prints:IN-FAMILY:PR00455 , Prosite:IN-FAMILY:PS01081 , Prosite:IN-FAMILY:PS50977

In Paralogous Gene Group: 512 (2 members)

GO Terms:

Biological Process: GO:0042493 - response to drug Inferred from experiment [Hirakawa08]
GO:0045892 - negative regulation of transcription, DNA-templated Inferred from experiment Inferred by computational analysis [Yang95, Hirakawa08]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred from experiment Inferred by computational analysis [Yang95, Hirakawa08]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11, GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: cell processes protection drug resistance/sensitivity
information transfer RNA related Transcription related
regulation genetic unit regulated operon
regulation type of regulation transcriptional level repressor

DNA binding site length: 24 base-pairs

Symmetry: Inverted Repeat

Consensus DNA Binding Sequence: TACATACATTtgtgAATGTATGTA

Regulated Transcription Units (1 total): ?


Transcription-unit diagram

Essentiality data for envR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 09-Jan-2009 by Gama-Castro S , UNAM

Sequence Features

Protein sequence of EnvR DNA-binding transcriptional repressor with features indicated

Feature Class Location Citations Comment
Conserved-Region 10 -> 70
UniProt: HTH tetR-type;
DNA-Binding-Region 33 -> 52
UniProt: H-T-H motif; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b3264 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11741; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hirakawa08: Hirakawa H, Takumi-Kobayashi A, Theisen U, Hirata T, Nishino K, Yamaguchi A (2008). "AcrS/EnvR Represses the Expression of the acrAB Multidrug Efflux Genes in Escherichia coli." J Bacteriol 190(18):6276-9. PMID: 18567659

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Pan94: Pan W, Spratt BG (1994). "Regulation of the permeability of the gonococcal cell envelope by the mtr system." Mol Microbiol 11(4);769-75. PMID: 8196548

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yang95: Yang K, Han L, Vining LC (1995). "Regulation of jadomycin B production in Streptomyces venezuelae ISP5230: involvement of a repressor gene, jadR2." J Bacteriol 177(21);6111-7. PMID: 7592375

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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