Escherichia coli K-12 substr. MG1655 Polypeptide: bacteriophage N4 receptor, outer membrane protein

Gene: nfrA Accession Numbers: EG11740 (EcoCyc), b0568, ECK0560

Regulation Summary Diagram: ?

Regulation summary diagram for nfrA

Isolation of spontaneous N4 bacteriophage-resistant mutants of E. coli K-12 strain SC1100, cloning of the affected genes, and subsequent complementation experiments revealed that nfrA is important in N4 adsorption. The 96 kDa nfrA gene product was found to be associated with the outer membrane in SDS-PAGE of outer-membrane fractions, though maxicell analysis found a small amount of NfrA associated with the inner membrane along with its 98 kDa precursor. This may be due to inefficient processing and transport of NfrA in maxicells. A mutation in the nfrB gene, directly upstream of nfrA and also important for N4 adsorption, did not affect NfrA transport to the outer membrane [Kiino89].

Gene Citations: [Morozov94]

Locations: outer membrane

Map Position: [587,205 <- 590,177] (12.66 centisomes, 46°)
Length: 2973 bp / 990 aa

Molecular Weight of Polypeptide: 111.31 kD (from nucleotide sequence), 96 kD (experimental) [Kiino89 ]

Unification Links: ASAP:ABE-0001939 , CGSC:31154 , DIP:DIP-10332N , EchoBASE:EB1691 , EcoGene:EG11740 , EcoliWiki:b0568 , ModBase:P31600 , OU-Microarray:b0568 , PortEco:nfrA , PR:PRO_000023375 , Pride:P31600 , Protein Model Portal:P31600 , RefSeq:NP_415100 , RegulonDB:EG11740 , SMR:P31600 , String:511145.b0568 , UniProt:P31600

Relationship Links: InterPro:IN-FAMILY:IPR011990 , InterPro:IN-FAMILY:IPR013026 , InterPro:IN-FAMILY:IPR019734 , InterPro:IN-FAMILY:IPR025137 , Pfam:IN-FAMILY:PF13283 , Prosite:IN-FAMILY:PS50005 , Prosite:IN-FAMILY:PS50293

GO Terms:

Cellular Component: GO:0009279 - cell outer membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Kiino89]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: extrachromosomal prophage genes and phage related functions

Essentiality data for nfrA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of bacteriophage N4 receptor, outer membrane protein with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 27
UniProt: Non-Experimental Qualifier: potential;
Chain 28 -> 990
UniProt: Bacteriophage N4 adsorption protein A;
Repeat 81 -> 114
UniProt: TPR 1;
Repeat 612 -> 645
UniProt: TPR 2;
Repeat 646 -> 679
UniProt: TPR 3;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b0568 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11740; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kiino89: Kiino DR, Rothman-Denes LB (1989). "Genetic analysis of bacteriophage N4 adsorption." J Bacteriol 171(9);4595-602. PMID: 2670887

Morozov94: Morozov GI, Nosova LIu, Biketov SF, Valiaev AG, Domaradskii IV (1994). "[Biochemical bases for the effect of combining kanamycin and nitrofuran resistance genes in Escherichia coli cells]." Mol Gen Mikrobiol Virusol 1994 (2);11-4. PMID: 8183300

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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